FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494875
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15140.10127937118488167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5300.049.2593881
CGAAAGC6650.040.337753
GCGAAAG7900.034.874072
TATACAG13900.027.6434785
GTATAAA9250.026.6562791
TACCGTA1100.00967378126.3632477
CAGCTCG11500.025.8465799
GTATATA22900.025.3347631
CCTCGCG3251.4824764E-924.545488145
GTATTAG6800.024.5290811
TATATAC22900.023.744193
TACGCCC2202.2300135E-523.067844
GTATTAC12000.022.3605671
TACTATA3004.5572233E-721.7504042
ATATACT11700.021.6877144
TCGCGCC2353.7121405E-521.5947049
TATTAGA11450.020.8955862
TATAGAC11000.020.4322012
TATACTT13900.019.8198535
TAGTACT8450.019.7334374
Produced by FastQC (version 0.11.5)