FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917922
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.052.467311
GTATAAA6650.031.6288991
ATAGACC5050.031.5861743
TAGACCT5100.029.8548474
TATAGAC6750.029.0034332
GCGAAAG4700.027.7692432
CGAAAGC4550.027.0896423
ATATTAG4650.026.5157361
GTACGCA1100.0096681726.365326
CCGGACT2507.812014E-826.105934145
CTAGTAC2507.824383E-826.1016673
GTATATA14100.024.6904981
TAGTACT3551.546141E-1024.5086084
TAACGAC2701.6475497E-724.1682113
GTATTAC7450.023.3648341
GTATATG4103.8198777E-1122.9967921
ACGACTG2903.2834214E-722.5014365
GTATCTA3905.002221E-1022.3164121
ACCGTTC1952.6153142E-422.3066868
TATACGT1650.002599734321.97114
Produced by FastQC (version 0.11.5)