FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997569
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAAAAT65430.655894479479615No Hit
CATGAGGGCTGTGCTTGCAGCAGGGTTTCACAAGACAAGGCACCCAGATT47460.47575656420758866No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC47410.4752553457455073No Hit
CTATAGAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCATGCT46500.4661331697356273No Hit
GATTAATAAACAGCACTTCAAAATTAACCGCATGAGGGCTGTGCTTGCAG45530.4564095315712497No Hit
ATCATACACTAAGATTAATAAACAGCACTTCAAAATTAACCGCATGAGGG43330.4343559192396716No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA39270.39365698011866845No Hit
CTCCTATCCGGACACTGATGTTATACTGATGTGTTTCTCCATTGACAGCC37050.3714028804022579No Hit
CTCTATAGAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCATG31610.3168703117278103No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA29630.2970220606293901No Hit
ACTAATACCTGGTCAGCCTTCTCATGTTCACTTGTAGAAATGAGCATCTC29550.2962201110900599No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC29510.2958191363203949No Hit
GATGTTATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAAAA28560.2862959855408498No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC26530.26594651598034824No Hit
GTAGAGAGGCAGGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAGCAGAG26380.26444286059410427No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT25360.2542180039676453No Hit
GATTATGACCGCTTGAGGCCTCTCTCCTATCCGGACACTGATGTTATACT24810.24870460088475083No Hit
CTTTAGTGCAGGTACTTTAAGAAGGAAGACAAGCAGGTGCTATGAATCTC22920.22975854301807694No Hit
ATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAAAATATCCC22870.2292573245559956No Hit
CACTAAGATTAATAAACAGCACTTCAAAATTAACCGCATGAGGGCTGTGC22640.22695171963042154No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG22320.2237439214731011No Hit
ATTATAGATAAATGAAAAAGGCCAGTAATCATACACTAAGATTAATAAAC21960.2201351485461156No Hit
CCCTGATAGTTTAGAAAATATCCCAGAAAAATGGACCCCGGAAGTCAAGC21210.2126168716148958No Hit
TCTAAATACTGGTAGCAAGATGACTTCTGATTTGTAATCTTAGGTAAATT21050.21101297253623558No Hit
GTAATCATACACTAAGATTAATAAACAGCACTTCAAAATTAACCGCATGA20990.21041151038173803No Hit
TCACAAGACAAGGCACCCAGATTTTTTCTTCCCACGTCTGGCTTGCAGAG18700.18745570481841356No Hit
GGTTTTAGGAGAGTACTTCGTTCTCTTGCCAGAACGACACTTGTCAATAA18660.1870547300487485No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG18470.18515009989283948No Hit
GGCCAGTAATCATACACTAAGATTAATAAACAGCACTTCAAAATTAACCG18360.1840474192762606No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT17910.17953645311752872No Hit
GTACAGGGTCTGATGAATGAGGGACGGACCAGAATTTGTTTAACAGTGGA17470.1751257306512131No Hit
CTACCGGTGGGATCTGGAGGGACCTGGAGTGACCAGGACCAGCAGCTGGC17430.17472475588154804No Hit
CTTCAAAATTAACCGCATGAGGGCTGTGCTTGCAGCAGGGTTTCACAAGA17400.17442402480429925No Hit
GAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCATGCTGTCAC17110.1715169577242276No Hit
GCTTTAACGCGAGAACCACCGCCCTCTACCGGTGGGATCTGGAGGGACCT16950.16991305864556738No Hit
GCCATGCTGTCACTTTAGTGCAGGTACTTTAAGAAGGAAGACAAGCAGGT16880.1692113527986535No Hit
CACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCAGCAGGAAC16730.16770769741240957No Hit
CAAGTAGAGAGGCAGGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAGCA16630.1667052604882469No Hit
TCCCTAGACTTATTGTGGTGATCGTTTTGCAGTGGATACAGATACCAAAT16520.16560257987166802No Hit
CTTCTGTCCCAACGTGCCCATCATCCTGGTTGGGAACAAGAAGGATCTTC16400.16439965556267286No Hit
CTGCCATGCTGTCACTTTAGTGCAGGTACTTTAAGAAGGAAGACAAGCAG16200.1623947817143476No Hit
CCCCGGAGGTGCCCTAGGAGCTAATTCACATCTTGGAAGTGCTCTAGGAA15930.15968820201910844No Hit
CCTCTAACCTCAGCTGTCCGTCAAGAGCAGCCGTTTCCCCTGTCCCGTAG15850.15888625247977836No Hit
TTAATAAACAGCACTTCAAAATTAACCGCATGAGGGCTGTGCTTGCAGCA15830.1586857650949458No Hit
CATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATG15550.15587894170729044No Hit
GTCAGAGACATTCACTTGTCACCTTCAAAACTCATCGGCTGCTTCGGGGA15510.15547796693762536No Hit
GGATACAGCTGGGCAGGAAGATTATGACCGCTTGAGGCCTCTCTCCTATC15450.15487650478312778No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC15420.154575773705879No Hit
CTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCAG15400.15437528632104647No Hit
GATCTGGAGGGACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGC15150.15186919401063986No Hit
GTTTAGAAAATATCCCAGAAAAATGGACCCCGGAAGTCAAGCACTTCTGT15070.15106724447130976No Hit
GGTATTAGTAGTAAGATGTTAGTGCAACCTTAATAACACGAAACAATCAT14710.14745847154432426No Hit
CTCTGGCCCTTTGCAGTGTTTGGACTTGAATTTGTGGGACATTAGGTCAA14640.1467567656974104No Hit
TCCCAGGACCTTGGAATCATGACCTGAGCTGAAGGCAGCGGCTTAACCCA14100.14134360630693216No Hit
CAATAAGGACATCACGTGGGAACAAGAGAGGACCGAGCTCAGACTTCAGA14010.1404414130751858No Hit
ATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGTGCTGC13870.13903800138135808No Hit
GCTGGATGCAGAGCTTCTAGTAAGTTAACGGTCAGAGACATTCACTTGTC13870.13903800138135808No Hit
GAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTGGAGGGCGCCC13860.13893775768894182No Hit
GACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAAGGAGACCCCG13300.13332411091363106No Hit
CTTGAGGCCTCTCTCCTATCCGGACACTGATGTTATACTGATGTGTTTCT13130.13161996814255456No Hit
GTATTGAGGCTCGTCACAAGTATCCTATGTCAGTGACCATTTGTCTGCAT12850.12881314475489916No Hit
GTTTGGACTTGAATTTGTGGGACATTAGGTCAAAGTGCCTTTTGAGACTC12850.12881314475489916No Hit
ACCTTACCACATTCTAAAGAATCAATGATTGTTTCGTGTTATTAAGGTTG12830.12861265737006664No Hit
GTCCCAACGTGCCCATCATCCTGGTTGGGAACAAGAAGGATCTTCGGAAT12640.12670802721415764No Hit
GGTCTGACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGG12460.12490364075066487No Hit
GACAAGTAGAGAGGCAGGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAG12360.12390120382650223No Hit
GGCCTGGAGCCCTCAATAAGGACATCACGTGGGAACAAGAGAGGACCGAG12190.12219706105542574No Hit
GGATACAGATACCAAATCATTACGGTAGACACCAGAACTCATAGAAGGTT12020.12049291828434924No Hit
CTCTCACCCCGGAGGTGCCCTAGGAGCTAATTCACATCTTGGAAGTGCTC11700.11728512012702881No Hit
TCTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCA11670.11698438904978001No Hit
TCTTACTACTAATACCTGGTCAGCCTTCTCATGTTCACTTGTAGAAATGA11590.11618243951044992No Hit
TCACTACGCTGAGCCTGTCCACGCCCCGGACACAATGATCAATTTCCGGA11520.11548073366353605No Hit
GTCCCGTAGGGTATTGAGGCTCGTCACAAGTATCCTATGTCAGTGACCAT11490.11518000258628727No Hit
GACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTG11390.11417756566212464No Hit
GTCTGGTGTGCTGCACACGGACAGCTGGATGCAGAGCTTCTAGTAAGTTA11290.113175128737962No Hit
ATCCCAGGACCTTGGAATCATGACCTGAGCTGAAGGCAGCGGCTTAACCC11280.11307488504554572No Hit
CCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGC11220.11247342289104814No Hit
CTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGTGCTGCTGTCTGGCT11210.11237317919863188No Hit
GGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTGGAGG11170.11197220442896681No Hit
CTGGAAGGCTGATCCCTCTGGCCCTTTGCAGTGTTTGGACTTGAATTTGT11130.11157122965930176No Hit
GTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTGGCCTGGAGTAACTGG11130.11157122965930176No Hit
TCTAGTAAGTTAACGGTCAGAGACATTCACTTGTCACCTTCAAAACTCAT11100.11127049858205297No Hit
GAGTTGGCTTTGTGGGATACAGCTGGGCAGGAAGATTATGACCGCTTGAG11040.11066903642755539No Hit
CTCTTGGGGCGGTGGAGGGCGCCCTGGCTTATCCTGAGGCTCCTAACCTG10880.10906513734889516No Hit
GTGGAGGGCGCCCTGGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAG10830.10856391888681384No Hit
CTGATGTTATACTGATGTGTTTCTCCATTGACAGCCCTGATAGTTTAGAA10810.10836343150198131No Hit
TGGCAAAGGAGACCCCGCACGGCAGTCTGGTGTGCTGCACACGGACAGCT10730.1075614819626512No Hit
CCAGTAGACGACCTTACCACATTCTAAAGAATCAATGATTGTTTCGTGTT10380.10405295272808196No Hit
TCTCTATAGAAATAGCCCAATTAAAAAATGAAAAATGGACGCACTGCCAT10250.10274978472667054No Hit
CTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTGGC10240.10264954103425428No Hit
GCCCTGATAGTTTAGAAAATATCCCAGAAAAATGGACCCCGGAAGTCAAG10200.10224856626458922No Hit
TCCCTGGGCACTAGAGATGCTCATTTCTACAAGTGAACATGAGAAGGCTG10070.10094539826317778No Hit
CCCTTGAGAGCAGATGTGTGAGGCCAGCCTGTGTCTGCGAGCTCCGGTTG10060.10084515457076153No Hit
CACTACCACTGTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGA10000.10024369241626394No Hit
GGCTGGGAGCGGGGGAAGCAGGCTCCTTGCTGAGCAGAGGTCCGGATGCG9990.10014344872384769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3000.0106.360731
AGTAGAA700.093.2383041
CGAAAGC4050.078.7659763
GAGCGAA755.638867E-1177.353261
GCGAAAG4150.076.871862
TAGCCGA300.001935302972.5041352
GAGAGTA4500.067.663749
CTATCCC554.0614304E-665.9095464
TAGGAGA4700.064.784446
TATATTC1700.063.9710353
TTCCCCG350.00356357162.15575145
AATATAT1800.060.4322281
AGCCGAC606.805303E-660.4170883
CACTGTA502.0096752E-458.0033072
GCATATT2200.056.0203068
TAACGCG4700.055.5295145
GTAGCCG400.00604471854.389011
ACTAACG400.00604471854.389011
GGAGAGT5600.054.372658
ACGCGAG4850.053.812117
Produced by FastQC (version 0.11.5)