FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999441
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGGGCAAGGCTAGGTCTGAAGTGTGAGCTCAGCGAGGAGCCTCCCA53450.5347989526145115No Hit
ATATATAACAGTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGTCCTCT53360.5338984492331212No Hit
CTATAATTACAGTAGCTTTGCCTACCCTAACATGAAAGGTTATGCTAGGG34250.3426915645846028No Hit
GAATATACCCACTTTAAAACTTCATGCATCTAACTAGCTTACTTATCTGA24210.24223540959396303No Hit
GCCCTAGTGTGTTCTGAATCGGGCAGTGATGGGACCTGACGTCCGTGCTC19040.19050649312965948No Hit
TCCCAACACTGCCTGAGGGTAGAGATGGCAATAATTACTGGTGGATCCGA18800.18810515077928563No Hit
GTGCGGGCCACAGGGGGAATCTGGTTTTCCTCTTAGTGAGAGGAGAAGCC18380.18390280166613138No Hit
CCTTAACTGTGCCTCCGGCCACCCCATCCTTTGCCTCAGTTGCTGGGCAA18050.1806009559343673No Hit
AACCTGGACCGTACCCCCGACCCCAGCTCCTTAACTGTGCCTCCGGCCAC15990.15998943409365834No Hit
GTTATATAGATTATGAGACAAAGGATGGCCTCAGCTGCTCTGGAGGTTAG15990.15998943409365834No Hit
CCATAGGAGGCATCAGGTATATTCTTGGGAAACAAAACATAGCAAAGTGA14870.14878316979191367No Hit
TCATAAAGCCATCCAGCAGCTTCCTTGGAGAACCACGTTCACCCTGACCT14450.14458082067875944No Hit
CTCCTTAACTGTGCCTCCGGCCACCCCATCCTTTGCCTCAGTTGCTGGGC13780.13787707328396573No Hit
GTTCTGAATCGGGCAGTGATGGGACCTGACGTCCGTGCTCTGTGTAGGGC13630.13637623431498208No Hit
GTAGTAGGTGGTGTCCGCACATCTGGCTGACTTTCACTGAAGATTGAGAG13140.13147349368296876No Hit
CCCCAGTGCTGTCCAGTTGTGTCACAAACTCACTACATGACTTCAGGGAA13060.13067304623284418No Hit
GGTCTGAAGTGTGAGCTCAGCGAGGAGCCTCCCACAGGCATCTAGGCTGC12890.12897209540132934No Hit
CTTATATATAACAGTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGTCC12750.12757131236361124No Hit
GTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGTCCTCTGCCATCCTTC12560.12567024966956528No Hit
GGGTAGAGATGGCAATAATTACTGGTGGATCCGAGGTTGAACCCGATGGC12390.12396929883805047No Hit
GGTATATTCTTGGGAAACAAAACATAGCAAAGTGAGCATCAAATAACATT12290.1229687395253947No Hit
GCTCCTTAACTGTGCCTCCGGCCACCCCATCCTTTGCCTCAGTTGCTGGG12050.12056739717502082No Hit
TCTCTGTACTTTATAATTTGGATATCTTGGCCTCCCACTCAGAATATACC11950.11956683786236506No Hit
GTCACACCCCGTGTCAGGCCCCTGGCTTTTGCTTCCAGACCTCTTAAATG11940.11946678193109948No Hit
CTCTTATATATAACAGTCCAGGTTAGGTGTTCATTTTGGCAACAAATTGT11760.1176657751683191No Hit
CTTTAAAACTTCATGCATCTAACTAGCTTACTTATCTGATTTATTGGCCT11290.11296314639883695No Hit
CCCTTTATTCTTCTCTTTCTTTTCCTGGAACACAGCAAGTCCCAAGATCA11210.11216269894871232No Hit
ATCCTAGGGAGATGTTATATAGATTATGAGACAAAGGATGGCCTCAGCTG11110.11116213963605656No Hit
TCCGTGCTCTGTGTAGGGCAAGGCTAGGTCTGAAGTGTGAGCTCAGCGAG10660.10665962272910558No Hit
ATGTTATATAGATTATGAGACAAAGGATGGCCTCAGCTGCTCTGGAGGTT10620.10625939900404326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2250.0125.689691
TAATTGG353.4384175E-582.851594
AGACCCG651.5643309E-978.07175
GCGAAAG3650.077.464452
CGAAAGC4100.068.95883
TATATAA17850.056.455632
CAGTCCA14950.056.2504169
AACAGTC16100.051.782257
ATATAAC16800.051.350733
TATAACA16850.051.198354
ATATATA18050.051.0204241
GTTATAG604.940613E-448.342191
ACTACGT1800.048.33018
GTTAGCC2850.045.7864079
GCACTAC1900.045.7864076
TACGGGA1002.7269361E-643.516685145
AGTTAGC3000.043.497098
ACAGTCC19600.042.5354238
CACTACG2100.041.42587
CTTATAT6900.039.9348531
Produced by FastQC (version 0.11.5)