FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_E08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_E08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522239
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG39660.2605372743701876No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT35100.23058140016120993No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT33310.2188224056800542No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC31970.21001958299583706No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30720.20180799467100766No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC28800.18919499500406967No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC28790.18912930229747102No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA28770.18899791688427375No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG28240.18551620343454608No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA26350.173100281887404No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC24500.1609471311666565No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC24190.15891065726209877No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG23610.15510048027937795No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA22680.14899105856570485No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20990.1378889911505355No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20900.13729775679114778No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20810.13670652243176004No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20530.13486712664699826No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20020.13151679861046786No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT19750.12974309553230473No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19090.12540737689679476No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT18560.12192566344706712No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG17870.11739286669176129No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT17740.11653886150597903No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17730.11647316879938038No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG17730.11647316879938038No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG17720.11640747609278175No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG17450.1146337730146186No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT17270.11345130429584317No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG17180.11286006993645545No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA16960.11141483039128547No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA15620.10261200770706833No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC15490.10175800252128608No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA15390.10110107545529973No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC15320.10064122650910928No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT15310.10057553380251065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG100.0070844386145.04643145
CCTATGA151.2285626E-4145.046431
AGTAGAA4200.0105.3313451
CAGGTAC3100.0100.563673
GGTACTG3250.093.691525
GCAGGTA3700.086.221152
GCATTTA406.653741E-572.52322145
GTAGAAA8200.072.523221
AGCGAAA1750.070.4511261
GCGAAAG1800.068.476142
GACAGTA5350.067.751997
CCTACTA1300.066.9225165
CGAAAGC1900.064.8678743
CACCTAC1350.064.44393
AGGTACT5150.063.348984
TTATAGG10000.063.0951961
AGCGAGC350.003562339662.162758145
GTGTTTT3850.062.1382569
CCACCTA1500.058.0033232
AGGGTGA1007.203198E-1057.999526
Produced by FastQC (version 0.11.5)