FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355400
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT33930.250332005312085No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA22720.1676257931238011No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17640.1301460823373174No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC15820.11671831193743544No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA14880.10978308986277115No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14710.10852884757267228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG300.001936235272.496768
AGCGAAA3550.053.117771
ATATCGC752.5517431E-548.331176
TATAGAC16350.036.8039932
TATATCG1001.392077E-436.2483755
ATAGACC18150.034.3510783
GTATTAC15650.033.8301051
TAGACCT19100.033.0220834
CGAAAGC6650.032.706512
TAGGTCG900.003629478632.220785
GCGAAAG7100.030.6448671
GAGCGTA1000.00607653828.99879
TATACAG13050.028.8875965
TTACATC17250.027.7378884
TACATCA16800.027.1862835
AGACCTC23550.026.47445
CCTCTTA24300.025.6572888
ATACAGC16200.025.5081166
ATTACAT19300.025.1672653
CAGGTTA13000.025.095039
Produced by FastQC (version 0.11.5)