FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960209
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.081.60721
CGAAAGC5250.037.2867163
GCGAAAG5400.036.2509772
TATACGG1000.00607353829.0007842
GTATACG1358.056652E-426.8665681
TATACTG4650.026.5060925
GTATAAG3850.026.3780821
TATACTA3306.184564E-1126.3643475
TAAAGTA7650.024.6411884
TATAGAC5150.023.9326822
CTATACT5050.022.9709174
CATATAC6350.022.8471581
GTATACC1600.002168172722.6686651
TATAAGA2953.8730468E-722.119242
GTATAAA7900.022.0373861
TCTAGGC7800.020.4492683
GTCTAAT5700.020.3620281
TAGGCTT4652.055458E-1020.2693635
CTATAAC1800.004306277720.1394313
TATATAG3259.826326E-720.0774633
Produced by FastQC (version 0.11.5)