FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105944
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG13640.12333355034251284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.059.153221
GCGAAAG6800.034.116372
TATACAG8850.033.5863155
CGAAAGC7000.033.1416173
GTTATAT3750.029.004161
TATATAC17600.028.422233
ATATACT10450.027.7501584
CAGCTCG9250.026.6476529
ATACTTA10350.026.6173616
TATACTT11850.026.3070345
GTATATA16450.026.006771
ATATATG8500.023.028553
TATATGT9700.022.421844
CATATAT12850.022.0070481
GTACTAC1650.002598867321.9728471
CTTACAC11100.021.553259
CTATAGT3055.343136E-721.3926434
CGGTAAA1700.003090426321.326591
TACTTAC12350.021.1328127
ATACAGC11450.020.8944136
Produced by FastQC (version 0.11.5)