FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836818
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAATGCAATCCAATCTTACAACCCTGAAATCACAATCCGAACCAAAA47850.5718089238042203No Hit
GTGTGGGACAGTGAAGAAACCGTCTTTCTTCCTCTTCTTACGGAATTCCT41440.4952092330709904No Hit
GTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAATGAGAG40640.4856492092665311No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG33060.3950679837192795No Hit
GTGAAGAAACCGTCTTTCTTCCTCTTCTTACGGAATTCCTGTCCATCAAG30490.36435640724745405No Hit
GTTGTAGGCAGTGTCTCCTGTGAGACCAAGGACCTGTTTGCTGCCCTGCC29570.3533623798723259No Hit
GGTGTGGGACAGTGAAGAAACCGTCTTTCTTCCTCTTCTTACGGAATTCC25860.309027769479146No Hit
GTAATGAGAGCTTGAAATAAACCCAAGGGCCTCAAATACCACACTAACAA24550.29337323049934394No Hit
GCACAAGGAGTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCATCAGA23740.28369370639732894No Hit
GTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAATGAG22820.27269967902220077No Hit
GGCCTAGAGGTGGGGGTCTCAGGCCTACGGAAGGTGACCTGAATGGGCAG22550.2694731709881958No Hit
AAAGCAGGCAGTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGA21470.25656713885217575No Hit
GTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCATCAGAACCACACTT21300.2545356337937281No Hit
GTCTTGTGCAGGTGAGTCAGTACCATCAGAACCACACTTCGGGAAGACTG20730.2477241168330509No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC20540.24545361117949183No Hit
TCAATGACCTCGGCACCATCAAGCTCAGCTTGGAAGTCACATGGAGCCCC19110.2283650686290209No Hit
GGCTAGGTCCCTGAGCTAGATTTTCTTGACCCAGAGCCTCCACTGAAGCC18420.22011954809767476No Hit
GTGCTGGAGCAGGTGCAGGTGCTGGAACAGGTGCAAGAGCAGGAACAGGA18200.21749054155144848No Hit
CACTGATATGGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGGCCAGG18190.21737104125389273No Hit
ACCCAGGTGCACCTGATTTCAGAACTTCTAAAATTAGCCTTTGCATCTTT18040.2155785367905566No Hit
CCTATAGCCAGAGCCCACCAGACACACCCTCACTTCGGGAACAGGCTTTT17540.20960352191276957No Hit
GCTGAGCACGGATCCCAATGCAATCCAATCTTACAACCCTGAAATCACAA17340.20721351596165474No Hit
GGACTGTAATGAGAGCTTGAAATAAACCCAAGGGCCTCAAATACCACACT16650.1989679954303086No Hit
ATATTAATCTAAAGCATGAGAGAACTCTTAATTTTAATCATGACATTGTG16460.19669748977674956No Hit
GCCCCAAGTGGTAGCAGTGGACATCAATGACCTCGGCACCATCAAGCTCA15820.18904947073318212No Hit
AAATAAACCCAAGGGCCTCAAATACCACACTAACAAGTCAAAAATTAATT15640.1868984653771788No Hit
GTGCAGGAGCAGGGACAGGTGCTGGAGCAGGTGCAGGTGCTGGAACAGGT15260.18235745407006063No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA15100.18044544930916878No Hit
GCAGTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAAT14630.17482893532404895No Hit
GCCCTCAACTGCTTCCACTGTCAACAAGGCCTCCACAGTCACCAAGCGCT14370.1717219275875997No Hit
AGCAGGGAGAGAGGGAAAGAAGCAGATCCCCTGCTGAGCACGGATCCCAA14120.16873442014870618No Hit
ATATAGTACAGTTTTTTCTCTTTTTTTGAGAGAGAGAGAAGAGAGAGCAG14010.16741991687559302No Hit
TGCTTCAGCAGTCCATTTCAGACTCCTCTGGTAGTCCATCACAAGCCATG14010.16741991687559302No Hit
CTCTTCATCCACGGGCCTAGAGGTGGGGGTCTCAGGCCTACGGAAGGTGA13950.16670291509025859No Hit
ACTGTAATGAGAGCTTGAAATAAACCCAAGGGCCTCAAATACCACACTAA13940.16658341479270283No Hit
GATCTGGACTTGGGTTTTTTTTTGTTTTGTTTTGTTGTTGTTTGTTTGTT13860.16562741241225692No Hit
CCTCTGGTAGTCCATCACAAGCCATGGAAGCCATCATGCCTTCCATTCCC13740.164193408841588No Hit
CTTATCTGACTGAGCCACCCAGGTGCACCTGATTTCAGAACTTCTAAAAT13670.16335690675869782No Hit
GATTATTATATATGTTTTGCGGGTGAGAGGAGAACAGAAAACTAAGGCTA13490.16120590140269447No Hit
GGCAGTGTACTGGGCCACCGATAGAGGAGAAATCAGCAACAGGACTGTAA13200.15774039277357801No Hit
GGTTTAATGAGCATATTAACTTTAAAAAGGAATATCATTTAAATAGCAAA12820.15319938146645984No Hit
GTAGGGGGCATGCCCATTGGCCAGGGCTGGGGCTTTGGCCCCCTCTTCAT12750.15236287938356965No Hit
GTATATAGCAGGCAACTAAGGAAAAGCTAACTAGCCTGTATACAAAGGTT12730.15212387878845818No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC12580.15033137432512206No Hit
GGCCAGGGCTGGGGCTTTGGCCCCCTCTTCATCCACGGGCCTAGAGGTGG12470.14901687105200892No Hit
CTACATGCCAGGCAATATAGTACAGTTTTTTCTCTTTTTTTGAGAGAGAG12350.14758286748134003No Hit
AAAGCAGGGAGAGAGGGAAAGAAGCAGATCCCCTGCTGAGCACGGATCCC12040.14387835825711207No Hit
GAAAGAAGCAGATCCCCTGCTGAGCACGGATCCCAATGCAATCCAATCTT12030.1437588579595563No Hit
GAACTGAAGGGTCTGGCCAACCATGTGGTTGTAGGCAGTGTCTCCTGTGA11890.14208585379377595No Hit
AGTCAGTACCATCAGAACCACACTTCGGGAAGACTGATCTGGACTTGGGT11730.1401738490328841No Hit
GTCAACAAGGCCTCCACAGTCACCAAGCGCTTCTCCACCTATAGCCAGAG11720.14005434873532835No Hit
GTATTACTCAGGCAGAAGAACCAGCTGGAGCGAAGGAAAGGCTGCAGAAG11600.13862034516465946No Hit
ATCCAATCTTACAACCCTGAAATCACAATCCGAACCAAAAGGCTTCAAGG11290.1349158359404315No Hit
TGTGAACACAGATCGCCACGGTCCCTCACGAGACAGACAGACCCACGGTC11280.13479633564287577No Hit
GGGCTGGGGCTTTGGCCCCCTCTTCATCCACGGGCCTAGAGGTGGGGGTC11200.13384033326242983No Hit
TTCTTTATGAGACCTGGGCTTACATCGCAGCGTGTCTTCTTCACCATAAG11170.1334818323697626No Hit
GTCTTGAGGGCTGTAAATTGAAGAACTCCCTGAGGCCACAACGAGTTCAG10770.12870182046753298No Hit
GTCCCAGGACCGTGGGATCATGACCTGAGCTGAAGGCAGAAGCTTAAACG10730.12822381927731No Hit
GCATGAATGTAACCTTCATATGCACTGAGAAACCAAAGTGTGTGACTCAC10570.12631181451641812No Hit
CATCTGCACAGTGGCTAATGACTACGTCACGGACTTGTTAAAATTAAACA10550.12607281392130668No Hit
ATTTCAGACTCCTCTGGTAGTCCATCACAAGCCATGGAAGCCATCATGCC10520.12571431302863945No Hit
GCTAAGAACAGGAGAGGTAAAGGGCACGTTCTTTATGAGACCTGGGCTTA10350.12368280797019184No Hit
CTTTTATTGAGGGCTTACACTACATGCCAGGCAATATAGTACAGTTTTTT10010.11961979785329667No Hit
ATATAGTATCATGTCATCTGCAGATAATGAAAGTTTTACTTCTTTCTTAT9960.11902229636551795No Hit
CTACAGCACAGCATGAATGTAACCTTCATATGCACTGAGAAACCAAAGTG9930.11866379547285073No Hit
AGCACAAGGAGTCCCAGGATGGTCTTGTGCAGGTGAGTCAGTACCATCAG9880.11806629398507203No Hit
CATCAGAACCACACTTCGGGAAGACTGATCTGGACTTGGGTTTTTTTTTG9820.11734929219973757No Hit
GTACTATATTGCCTGGCATGTAGTGTAAGCCCTCAATAAAAGTAACTGCA9750.1165127901168474No Hit
CATTTAAATAGCAAAGAAATTTGACAATTGCAGTTACTTTTATTGAGGGC9720.11615428922418017No Hit
TTACTTATCTGACTGAGCCACCCAGGTGCACCTGATTTCAGAACTTCTAA9610.11483978595106702No Hit
CCACACTAACAAGTCAAAAATTAATTTGATAAGCACAAGGAGTCCCAGGA9560.11424228446328831No Hit
CTCAAATACCACACTAACAAGTCAAAAATTAATTTGATAAGCACAAGGAG9550.11412278416573257No Hit
GTGGTATGTCAAGCTAGTATTACTCAGGCAGAAGAACCAGCTGGAGCGAA9470.11316678178528664No Hit
GCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCT9350.11173277821461775No Hit
TCATGCTACTGTACACTTAACAGACTACAGCACAGCATGAATGTAACCTT9350.11173277821461775No Hit
GCCTAGAGGTGGGGGTCTCAGGCCTACGGAAGGTGACCTGAATGGGCAGA9230.11029877464394887No Hit
ATAATATGCTGAGGCGTCAGGAGGAGCTGGAGAATGGGACAGCATGGTCC9210.11005977404883738No Hit
GGTAAAGGGCACGTTCTTTATGAGACCTGGGCTTACATCGCAGCGTGTCT9190.1098207734537259No Hit
CCCCTGCTGAGCACGGATCCCAATGCAATCCAATCTTACAACCCTGAAAT9080.10850627018061275No Hit
GCTGTAGGGGGCATGCCCATTGGCCAGGGCTGGGGCTTTGGCCCCCTCTT9060.10826726958550127No Hit
CCTTCTAGGAAGTGGTTTAATGAGCATATTAACTTTAAAAAGGAATATCA9000.10755026780016681No Hit
TCTTACAACCCTGAAATCACAATCCGAACCAAAAGGCTTCAAGGCTTACT9000.10755026780016681No Hit
CTTCTAAAATTAGCCTTTGCATCTTTCCCCTTGCTTCAGCAGTCCATTTC8950.10695276631238812No Hit
ATAAAAAGCCTGTTCCCGAAGTGAGGGTGTGTCTGGTGGGCTCTGGCTAT8840.10563826303927497No Hit
GGCCTCACTGATATGGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGG8840.10563826303927497No Hit
GTACCATCAGAACCACACTTCGGGAAGACTGATCTGGACTTGGGTTTTTT8830.10551876274171922No Hit
ATAGAGGAGAAATCAGCAACAGGACTGTAATGAGAGCTTGAAATAAACCC8740.10444326006371756No Hit
GATATGGCTCAGAGTCCGGCTGTAGGGGGCATGCCCATTGGCCAGGGCTG8730.10432375976616182No Hit
ATCAAGCTCAGCTTGGAAGTCACATGGAGCCCCTTCGACAAGGATGACCA8650.10336775738571588No Hit
GAACAGGAGAGGTAAAGGGCACGTTCTTTATGAGACCTGGGCTTACATCG8640.10324825708816014No Hit
ATATTGCCTGGCATGTAGTGTAAGCCCTCAATAAAAGTAACTGCAATTGT8590.10265075560038145No Hit
GGCTCAGGCTGCTGCACCAGAGGCCTGGGGGCTGAAGAGTGCCGGGCAGT8580.10253125530282571No Hit
GGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTG8540.10205325411260274No Hit
ATACCACACTAACAAGTCAAAAATTAATTTGATAAGCACAAGGAGTCCCA8460.1010972517321568No Hit
ACCATGTGGTTGTAGGCAGTGTCTCCTGTGAGACCAAGGACCTGTTTGCT8450.10097775143460108No Hit
GTGTAAGCCCTCAATAAAAGTAACTGCAATTGTCAAATTTCTTTGCTATT8400.10038024994682238No Hit
GATCCCCTGCTGAGCACGGATCCCAATGCAATCCAATCTTACAACCCTGA8370.10002174905415515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4800.0122.379851
AATACTC406.116734E-790.6246646
ATACTCA406.1188985E-790.619257
CTTATTC259.386347E-486.999683
GCGAAAG6800.086.37032
CGAAAGC7400.079.357823
GGGGAGC406.654788E-572.51707145
TAGGGGT7650.069.182754
ATAGGGG7700.068.733513
GTGGCGA7850.067.416119
GAAAGCA8800.066.732714
AATTCTT350.003562976662.157482145
AGCCTCG350.003562976662.157482145
GCTAGGT3750.061.8738372
GGGTCCG502.0077167E-458.01365145
ACAGTGA12100.056.9178778
AGGGGTG9450.056.7722785
TAGGTCC4200.055.237894
CTAGGTC4250.054.5880363
GTTCTGG805.860584E-754.391051
Produced by FastQC (version 0.11.5)