FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490349
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAAGTGGCCGGAGCGGGGGTAGGGCTGGTGGCCACCATGGTT12560.25614409328865767No Hit
CTTATAAAGTTTGTTGAATAAATGACCAAATTAGGCATGAAAAGATGATC10160.20719936208700332No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG8840.18027975992609346No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC7800.15907037640537658No Hit
GGATAAAGAAAAGGATACGCAAAAGAAGCGAACGGAAATTTCAGAGATAA7630.1556034579452594No Hit
ATTAAATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGC6840.13949248392471483No Hit
ATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGCTCCAG6800.13867673840468728No Hit
GATATGAACAATATTTGGTTCTAAAATGTGACTTGTCTTTAATTGGGAAA6640.135413756324577No Hit
TTCTAGTACTGAACAAATCTTGACTTTCTTTGGAAAAGCTGAACAAAAGA6200.1264405556042737No Hit
GTCTTTAATTGGGAAATGAAATATTGTTGTTAACTGCCCAACAACAGGTC5820.11869097316401175No Hit
CTATTAAGTCTGTTTTCACATTCGCCGCCTTCTCCTTTCCCAAGAGAAGG5420.11053351796373603No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG5400.11012564520372224No Hit
CTAATAATCAGTCCACTTTAAAAAAGTTAACATTTGAACCTCACAGGTCC5190.1058429812235775No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGAACACCAAGTCTGTGA5180.1056390448435706No Hit
GTTGTGTATCTTCCCTTCTATTAAGTCTGTTTTCACATTCGCCGCCTTCT5110.10421149018352235No Hit
CTTCAGATGGCTCCTTTTAAATTTCCAGTTGAACCACCCACATATTTTTT5100.10400755380351545No Hit
GCTCCAGTCCGTGCTATAGTCGGAACCACTGTGCAATCCTGCCAGCAAAT5010.10217212638345342No Hit
TTCTATGACTAAAAAGTGATGAAATGCAACTAACCAGCTAGTCAGCTTTG4990.10176425362343963No Hit
TATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTGAA4940.10074457172340517No Hit
ATGCTAGACTATGTGATGTTCCAGCAGAGATCTGCTGAATAGCTATGCCA4930.10054063534339827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1150.0119.812611
CGAAAGC1700.081.024913
GTCTACA651.3941462E-766.9398351
GCGAAAG2250.061.2250632
ATACCAT2650.060.1853336
ACTATAT1051.1077645E-955.2350233
TACCATA2550.054.0166057
TACTATA1203.612513E-948.335582
TAGACCC450.00963834548.3306474
ATATAAT3350.047.6238631
CCATACG3050.045.1614239
AATACCA3550.044.9270825
ACGTGCG657.334919E-444.6129049
CTAGTAC1703.8198777E-1142.6446883
GGACCGT1557.385097E-1042.0944376
TATAGGT700.001055872341.4304962
TATAATG3000.041.085242
GTACTAA907.465824E-540.2878651
GATCTAC907.479304E-540.275547
TAGTACT1807.2759576E-1140.275544
Produced by FastQC (version 0.11.5)