FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_B08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_B08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267119
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC27410.21631748872836726No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA24810.19579850037762828No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC21190.16722975505852253No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18100.1428437265955289No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA18070.14260696903763578No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT16940.13368910102366074No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT16930.13361018183769638No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC16520.13037449521315678No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT16480.13005881846929926No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA16280.12848043475001164No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA15950.12587610161318707No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG15740.12421879870793508No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG15520.12248257661671871No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG15320.1209041928974311No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT13930.10993442604838219No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG13850.10930307256066715No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC13240.10448900221683995No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG12980.10243710338176604No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA12800.10101655803440718No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12770.10077980047651404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2200.079.111121
CGAAAGC3200.054.373883
GCGAAAG3250.053.5373542
GACAGTA4250.052.8791737
AGTAGAA7350.052.292281
TTATAGG6000.049.554331
GTAGAAA10800.047.674221
CAGCTCG3550.044.9268729
CTATACC657.3380745E-444.6144642
CCTTATA9600.043.801182
TTATACT10200.042.6461754
CAGGTAC9700.042.602213
TACGATT700.001056855541.4277153
GGTACTG10100.040.9149975
GTACGCT2850.040.7009163
CAATTCG5350.039.2967039
AGGTACT10900.039.242314
CCCTTAT10850.038.7656671
TACGCTC3050.038.0319984
GTGTACG3200.036.2592661
Produced by FastQC (version 0.11.5)