FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012059
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20670.2042371047537742No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19490.1925777054499787No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA19120.18892179210895807No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA18770.18546349570528992No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16080.15888401763138316No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC14460.14287704570583334No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG14090.1392211323648127No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA13640.13477475127438224No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC13360.1320081141514477No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG12470.12321416043926292No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12430.12281892656455799No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11840.11698922691266024No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10920.10789884779444678No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10620.10493459373415977No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10440.10315604129798757No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10350.10226676507990146No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC10210.10088344651843421No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10180.1005870211124055No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA10130.10009297876902433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2050.084.9202651
AGTAGAA6200.070.196191
GTAGAAA10900.048.5791971
GCGAAAG3600.048.333482
CGAAAGC3600.048.3310933
GTATTAC6200.047.9673961
CAATTCG6600.046.1342289
CCTTATA7800.044.6155242
TTATACT8550.044.0915264
CTGATCG1053.8201924E-641.434837145
GACAGTA4450.040.7284477
GGTACTG5750.039.0851445
CTACACT5800.038.7482034
TTTAAAC8300.038.4319533
TAGAAAC13350.038.01512
TACATCA6600.037.3467565
GGTCAAA7250.036.0179061
TATTACA7750.035.5484962
GCAGGTA6650.033.7970962
TCTACAC6700.033.543223
Produced by FastQC (version 0.11.5)