FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856713
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC34840.4066706119785739No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT24580.2869105523086495No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT21340.24909158609709436No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA20010.2335671339176597No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA18120.21150607029425256No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC17020.1986662978150209No Hit
CTTAACGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA11110.12968170204023985No Hit
ATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTG9850.11497432629130175No Hit
GTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATT9610.1121729213867421No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG9550.11147257016060219No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC9220.1076206384168327No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT9210.10750391321247607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.051.3691141
GTATTAC15700.038.3391841
GCGAAAG3650.035.75342
TATAGAC21400.035.5724032
ATTACAT15750.035.4444123
TTACATC16100.033.3229564
TATTACA17500.033.142832
ATAGACC12350.030.5262933
TAGCGAA950.0047282930.5245087
TACATCA17550.029.7435655
TAGACAT13150.029.220514
TAGACCT14250.028.9999774
ACATCAA18350.028.8419386
ATAGACA13550.028.3579123
TACTGGG19850.027.7581623
CATAGCG1050.00770787427.6190265
GATACTG20300.026.7935121
CGAAAGC4600.026.7934593
TAGGTAC27400.025.93972145
ATACTGG21000.025.8928362
Produced by FastQC (version 0.11.5)