FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S3_R1_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S3_R1_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022993
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG12510.1222882268011609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.053.3189431
CGAAAGC4250.042.6468163
ACGCTCG850.002742091734.117456
GCGAAAG5800.031.2528742
TATACAG9900.030.0250825
TATAGAC8800.029.661822
ATATATG7500.028.0331733
TTACGCA2603.7198333E-927.8844574
CAGCTCG7800.027.8830939
GTATATA15000.026.1049561
TATATAC15900.025.9904163
TACGCCC1450.001226503824.9998574
GTCTAGG2351.3282897E-624.6855351
CCAATAC5200.023.7017883
GTATTAC7350.023.6779631
ATATACA15350.022.198574
TACGCAG2953.8747748E-722.1185175
CTACGCC1650.002600986721.9695723
TATAGTA2704.4414774E-621.483462
ATAGACC6750.021.481363
Produced by FastQC (version 0.11.5)