FastQCFastQC Report
Thu 6 Mar 2025
HNCN3DRX5_n02_Petrov_10X.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCN3DRX5_n02_Petrov_10X.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323779997
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG31209070.9638974083998154Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAATAAGCGGAGGAAAAG12578580.3884915719484672Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATATCAATAAGCGGAG9267200.2862190402701128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA7255690.22409321351621359Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATATCAATAAGCGGAGGAAA5876100.18148434290089885Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGGAGGAAAAGAAACCA5222830.16130798839929572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA4763040.14710729643993417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAGTACCCGCTGAACTTA4753600.14681574044242146Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGAG4667950.14417042569803964Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCATATCAATAAGCGGA4020650.12417845565672792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGA3711960.11464451276772358Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)