FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_chi_092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_chi_092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAATTGTATATACAAATAGACAATAAAATAAACTAAATTTACAAATT17.142857142857142No Hit
ACCTCTGTTAGGACCAGACCAACTTCGACATCAACAATAAAATCCTGGAC17.142857142857142No Hit
CCATAGGAAGTATTTGTGAAATAACTTCGATGAGAAGAAAAAAGAGGGCG17.142857142857142No Hit
AATTGGTTATTAATAATTAGTATGTCTTAATGTTGGTTGCAGGTGATAGA17.142857142857142No Hit
ATATTACCAAACACAACACTATCAAACCTTACTATTATTCCTCAAAACAT17.142857142857142No Hit
CAAGTTTTCTTCTTATCCAATGTGGAACGAGTTAGCCCAAAAAAAAGACT17.142857142857142No Hit
AATATAGTGAAAAAAAAAAAACATTAAAAATGTTGAGGAAATATTAAAAT17.142857142857142No Hit
ATATTGGACTNCAAAAAGCAAATAAAGGATTAATGAACTGCTTGGAAATG17.142857142857142No Hit
CCTCTGACTTTACTTTGTTCAAGAAACTTGACTCCATTCCATGGTTTACA17.142857142857142No Hit
ATNAAGAAACAATTTAAGCTTGAGAAAGTTTTAGTCTACAAAATAGGTAG17.142857142857142No Hit
CTTCAGAACGTCGTTTTACACGTGGATATGGCAAGAAACAAACTTGATCT17.142857142857142No Hit
TTATAACACCCTTTATCATTTATTATTAAATAGATGGGAGTAATAAACAT17.142857142857142No Hit
ATCAAGAATATTTAAAATTTCAATCATTCGCTTACAAATTAAATTCAGAT17.142857142857142No Hit
CAAACGGGCTAAGGAATCAAATCTGCATATTGGCCAAGGTATGTAACGAT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)