FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_chi_056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_chi_056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACATATTCCGNAAAAGAGTATTGGACAAACATGAGATTATACCTG12.941176470588235No Hit
GAGTTTTGATGGAAATTTTTATTCAATCTTATAGAATTGCTTATTTTATT12.941176470588235No Hit
ATACAGAACGGGTGATTGGAACCAAGGTACGAGAGCCTCGTGACCGAATT12.941176470588235No Hit
ATACCTTGGATCGCCGAAAACGCCGGCATTGACCGTGAGCTGGTCAATGA12.941176470588235No Hit
TAAGTAGTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATATGGAC12.941176470588235No Hit
AAGATAGAAGCNGTCACAAAGTGAAGCCATGCCCATATGAGTAGTGTGTG12.941176470588235No Hit
CCTATGGACTTTGGCTGCACCATGAAAGCTTTGAGAAGCAATAAGAAGGT12.941176470588235No Hit
GACTTATTTTTTTACTTACCAATTTATAAATGGGCTTCGATGTACTGAAA12.941176470588235No Hit
GTGTCCTGGGTAGAGATAGAGTTCTGTTTTGTATTTTCATTAGGGTTTGT12.941176470588235No Hit
TATTTACTCTTGTTTATTATTTACTATTTTCATTTTCTTAAAAACATTGA12.941176470588235No Hit
ATAGGGTCTGTGTTTTCTTCGGGTACTCGGTGAGGCCAGTCGGAGAGTGT12.941176470588235No Hit
TTGCCGGGTTGTCCACCTAAACCAGAGGCTGTTATAGACGCTATAACAAA12.941176470588235No Hit
ACTAGAAGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACG12.941176470588235No Hit
GTGTAAGACTCATAGAAGCTCGCTGAGAGAATTGCTAAGCTAACTGGAGG12.941176470588235No Hit
AGAATATTATGACTTTAGTGTGGGGGTTGTGGGTTGGGGGGGGCGGGGCT12.941176470588235No Hit
ATATCATATATNGTGATCTAATAGAACATGGTTTTTGATTCTTAAAACTA12.941176470588235No Hit
GCGATTAATAGTGCGGTTAACTGTTCGGTTACCATATAGTCTGTCTCTTA12.941176470588235No Hit
GAATACGACTGGGTTTGGCTGGGTGTCCAGCGCGGGGCGGGCGGGGCCGG12.941176470588235No Hit
AATACTAACACCTTGAACGGGATCAGGTTGTACGCCGGATCCACCGCCTC12.941176470588235No Hit
CAAGAAACCATATAAAGTTTCAAGTGTACATATATATAAAATTAGTTTTT12.941176470588235No Hit
AATAAATCCCAAGGCAACCTAGATGAACGGACAACAGATTCTGGAAAGAT12.941176470588235No Hit
CTTTAGGTCTTTGTGATGCAGTTAAGTACCCTGCAAGATCAAAACAAAAC12.941176470588235No Hit
GCCTNTTCCTTCGAATAGCAATGCATTCCTGTTTTTCCATAAAAACCAAA12.941176470588235No Hit
CCTTTAAGATCTGATTGCGGTTTAAGTTATTATACTCAATGATTCACATG12.941176470588235No Hit
AATAGGATTAAATAAAAGTGAAAAATAAACAAGAAAATAGTAGAGGCTAT12.941176470588235No Hit
CAATGATACACATGACATCAAGTCATATTCGACTCCAAAACACTAACCAA12.941176470588235No Hit
CGTTCTTCTGCATCTGCTTGTGGTTGTGAGGGGGGGAGTGTGGGGCTTGA12.941176470588235No Hit
GCTTTGAAAATTAAAAAGTTAAAACTTCTTTGAATTATGAAAAACATATT12.941176470588235No Hit
TCATTTGATTCCCCATAAAAAAATAATATGAATATCACAAAAACTATATA12.941176470588235No Hit
ATGTTATTACGTGTAACACCTTTTTGAATACTTTGAGCGAGAAGTCAAAT12.941176470588235No Hit
GGCTGTGCGAGACTCCGGAGGCAAAGCAGCGGTAAGCTCATCGGCACAAG12.941176470588235No Hit
ATTCAAAGAAGTCTCTAAGTTTCCTTGTTGGTGCTGTGTACAGTATTTGT12.941176470588235No Hit
AATTAACACCATTAAGATATAGTCTATAAATTTTACATAAAGAACTGGCC12.941176470588235No Hit
GTAATATGTTTATACATTAAAATTAAATTAATAAACTATTCTATTTAACT12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)