FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_chi_045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_chi_045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCAGTATTCACCTACACATAATATGAATATCATGTTTACAGAGTT16.25No Hit
CATCAACACAGAAAACAATCCAAAAAAATAAACCACCATCGACTAGCTTC16.25No Hit
ATTGTACCCAAAGAAACATAGGTGAAAACCTACCAAAAATCAAAGCAAAA16.25No Hit
GCAAATACCACTCTTTTTTGATCTTCCAAATCAGTCCCGCGGGAGATCCG16.25No Hit
AATACAACATAATCCGTCAAAGCTGAGAAGGATAAATATATTTTAAGATA16.25No Hit
GTAGAACATTCTGTTTTGTAAAAACGTAGTCTCTTAGCCGGTTAGATTGG16.25No Hit
TGGTGGAATTTGTCAAGGGGTGGGAACGCATATTGCCGGGGGAAACGTTG16.25No Hit
TTTCTCCCACAAACCGAGCTTATACCAACACGCTGCTCTGTTGCAGTACA16.25No Hit
GCGAACAACCGGAGCTATTACTCACTACATCAAAAAAATTTCCATTAATA16.25No Hit
ACATACATGTAAACGAATCATTGAAACTGCAGACAAGAAGTGCTGATGAA16.25No Hit
GTTCTTGGCAACCATCTTTTGATGGGAGTCCGGCTGTTCGATAGCCGGCC16.25No Hit
GTCTCAGAATAGCCTAACGAGCTTCTTATGGCTAACACTCCCAATAGCTA16.25No Hit
GCATTATAATCGTTTCCGTACATGCTTTAACATATAAAGAAACTCATAAC16.25No Hit
TCTGGTTACGTTCGCGACTTCCAAGTTCATCGTGTGGACTTGGGGGGGCG16.25No Hit
AATCCAAGGTCTTCAAGCATACTTGCCTTGAGTTTCTCTACCCTTTGACT16.25No Hit
GCATACTGGATCCTTTTTAAGGCCCATTATGTACATTTCTGTTGGACCAA16.25No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)