FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_chi_037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_chi_037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGCNGGGTCTTGGGGGGCGCGGGGGGCGCGGGTGGGGGGGCAGGTT10.8130081300813009No Hit
TTCTGAGCAAGATAATATAAAATAAATCTAATAACAAAACAAAAAACACA10.8130081300813009No Hit
GGACTGAGCCATTGCGTATGCACTTTCCGAGGCACAAGCCCACGCCTCGC10.8130081300813009No Hit
AAATCAATTCANTCAATATTTCCCTGGGTCGTGGTCCCAGTCGGTCCTTT10.8130081300813009No Hit
ATACGCATCGGAGTGTGACGAAGGGGAAGGCGAAGGCGTCCTCGACCCGC10.8130081300813009No Hit
CTCAAGACTATTCCATCTCCAAACGCATCTAAAACCACTACTACCAGCAG10.8130081300813009No Hit
CAATTGGCTAGAATCCGTTACTTGAACGAAACTAGATCTTGTAGAATCAT10.8130081300813009No Hit
CTATAACACAATCAACCGAACCTAAAGCTAACAAACATCCCTCTAAAACC10.8130081300813009No Hit
GAAACTAAGTGCTTATTATGTATATGATTCAACTCTGAAATGTTTCCTTT10.8130081300813009No Hit
CGCGAGGAAAGTAATCAAAAAAGAAAGAAGATGAGTTCTTCTTTACTTTT10.8130081300813009No Hit
TTCTCGAAAAAGAAAAAGAAAAAAAAACAGAGAACAGCGAAAGGAGCATG10.8130081300813009No Hit
ACCTTAAACCTCGGTCGCAACCGCTTCCCGCGCGTGGCCTTCTACCGCGC10.8130081300813009No Hit
CTATATCTATCTGGATATAGAGAAATTTAAATTAGTAAAGACCTCACTCG10.8130081300813009No Hit
GTTTCTGGACTTATCGTCTGCGGAGCAGTATAACCATCGCCAAGCTTCAG10.8130081300813009No Hit
AATACTCGATAGCTTAAATTCTATACAATCAAGAAGCCTCGCCGAAACAA10.8130081300813009No Hit
GAGGCCGGCGATGCGTCCCGGTCGGATGAGGAACGGAGCAATCCGGTCCG10.8130081300813009No Hit
ATACACTACTCAATGCCGTCATACTTCACCTATTCCATACATGACGGCTC10.8130081300813009No Hit
ATACAAACCGATATACAAACAACCAGATAGATCACACAAAAAAATAATAA10.8130081300813009No Hit
GTCTCTCTCTCTCGCTCTCGCTCTGTCTCTGTGGGTCTCTCTCTGTCTCT10.8130081300813009No Hit
AACAGCTGCCGCCACACGGCCGTCCTATCAGTAGAAAATCATGATCGGGC10.8130081300813009No Hit
GATCTACAATAGACACAATGAGATATGTAAATCGTTTTTAGAAAAACTAT10.8130081300813009No Hit
GATACCTAAACAAACAAACAAATTGGTATACATACAAAAACACGGGAGAA10.8130081300813009No Hit
AAATGGGAGCTGCTGGGCGCTCGTGTGGGCGCGGGGGGGGGGGGGGGGGC10.8130081300813009No Hit
GTACAGAACCACAGGAAAAAACATAGCAAGACATATCAACGTCATCAATG10.8130081300813009No Hit
TCCAATTTATCAACAAGTCGCTAAAAAGTCAGCCCTAGAAGTGGAGATCA10.8130081300813009No Hit
CATAAAGCACTTCCCCGGTAATCACGGGGTCCACAAGCCCTGGGTTAGCG10.8130081300813009No Hit
ATCTTCGGAGTTCGGAACCGACGTTATTGGGTCGCGGGGGGGGGTGTGGT10.8130081300813009No Hit
ATATCAACGTGAGGGAACTAAACTCTCATAAGACACTGGGCCCTTCTCCC10.8130081300813009No Hit
CTTTTGACTCGAGTTATAGAAAAACACAGATGCTGTAGATGCGGGCAGGT10.8130081300813009No Hit
TATATAAGGTGGTCGAAGATTGCGCAATATCTACCAGGAAGAAGCGAGCA10.8130081300813009No Hit
ATGAAAATTTTTATATATTAATTATTATAATTACGTATAACAAAATTATA10.8130081300813009No Hit
AGTCGCAGCCCTCCAGAGGAGGCATCTTGACAGGACCCTGGTGCGCGGGC10.8130081300813009No Hit
ATTATATGCAGTCCAATATCGCAATCAATAAAGGAACTAATGCTTGCAAT10.8130081300813009No Hit
GGACCAATGAATCATAAAAGTTGTGTGCATCGATGAATATAAATTCAATT10.8130081300813009No Hit
GTACATATACAAGCATACAACAACAATATGTTTAATTATAAGCTTTTTTT10.8130081300813009No Hit
GCTTCCGAGATTGTTGATGGAGGAGGAAGCAAAAAAGAAGGGGCAACGGT10.8130081300813009No Hit
GCCATCAATAAACCTCCATTCCAAATGAGGCCAAGGGAATTCGTCCTCAT10.8130081300813009No Hit
GATGCGGTTTGGGTGTGTAGACGAAAAAATAGACAAACGGTTAATGGTGT10.8130081300813009No Hit
ACGTTAATCATACCAACGTTGGCATTACGTTAATACTAAACCGACTAAAT10.8130081300813009No Hit
ATCTCTCGAAGTCACAATTCAAGTGATAAAATATACTTAATTAATGCCTC10.8130081300813009No Hit
AGTAAAGCAGAAATGAAACAAAAAAAGCTTTGTAAAAGAGGATTGGGGCT10.8130081300813009No Hit
AATAAAAATATATCATTAGTTAAAAGGATCTAGCAATCAAGTCGCAAATT10.8130081300813009No Hit
AAATTAAGTTTTCGTAAAATAATTACGGATGCTAAAAGTGAGTTTTAGTA10.8130081300813009No Hit
TCTTTACCCCATACAGAGAGGGAATAGAAGGAGCCACTTTTTTTTCCACT10.8130081300813009No Hit
GAGAAGCACTNGTCTCATCTGTTGATAAAATGATATTTTTTGGATCCACA10.8130081300813009No Hit
CTATTAGTTCTGGGCCGAGTCGAAGATGTCCAGCTGCTCCTTTGACACCT10.8130081300813009No Hit
CTCGTTACGCTCTTCGACTGAACTCGTTGGCTCCGCGTCCACCGACAGTC10.8130081300813009No Hit
TACCCCTGACATTACGATATTAGCCACCAAAACACAAACAAACCATCTTC10.8130081300813009No Hit
CAAAAGCACACACCACCTACCGACAACAAGGCCGACCGACAGACGAACAT10.8130081300813009No Hit
ATCCTTGAAACTAATCTTGTAATATAAAGGCGACTACTTTTCTATTTTTT10.8130081300813009No Hit
CTGCTTTCTCAACAGTAAGTTGCTGGTGTGGGATATTGTGATAGCTGGCT10.8130081300813009No Hit
AATTTCGACTGCTGTTTGAGTTTGTGTACTACCTCTGAGATGGGAATAGA10.8130081300813009No Hit
TTGTAAGACCACGACTGATCCTGAACGGACGGACTGGAAGAACTGGCCTG10.8130081300813009No Hit
ATGGAAAACAGACTAATAAAGAACTAGAAAACTCCGTCGATGTGGAGCAG10.8130081300813009No Hit
AAGGGTTCCTCTTAAGAGATTGTGATTGGTGTGTGTTGTAGTTGTGTTGT10.8130081300813009No Hit
ATTAAGTAATAGGTTGAAGTAACGAAATACAATCATTAATATTAAACTCA10.8130081300813009No Hit
AAAACTCTAGATTCACGTGACCACAAACAATGCAATAGCTTCATCGAAAT10.8130081300813009No Hit
AGTGTAGAGTTTACTTGCGATTTATATGTCATTTGAGTTTTTTTGGGTGA10.8130081300813009No Hit
GTAATTCATTACCTGAGAATCAACGACCCAACAAAATCGAATCAAAAGTA10.8130081300813009No Hit
ATGCCGATCCTTGACGTCTGTTCTGTCCGTCCCGGGGACGTTCTGGCCGG10.8130081300813009No Hit
GAGTGAAAAGGCTCCAAATTCTGAGGGTACATTGGTTGATGTGGGTTTAA10.8130081300813009No Hit
GGGGGCGTGGGGGGTTCGGGAGTTTGTTGGAGGAGCTGGGCTTGGGGTTT10.8130081300813009No Hit
GGTTTTTAATAAATACAGAAAAAAAACCGCAAAAATTTATCATAACAATT10.8130081300813009No Hit
GTGCGCAATGGTGGTGAAGAAACGATTCTTCCTCTCCTCCGGGATTGGCC10.8130081300813009No Hit
GTGTTTGGGAGTCGAATATGACTTGATGTCATGTGTATTATTGAGTATCA10.8130081300813009No Hit
GGGCCGGGAGCTTATTTGCGGGTGCCTGGGAGGTGAGGGCGTAGTTAAAT10.8130081300813009No Hit
CTAACTAAGTTAAACAACAAACTATACTAATAACGAGTTAAGATCCAACG10.8130081300813009No Hit
CTCCGGCCACCGAAGGGACCAAGACGTTTGAGCGGCTGACCGATCCCGGC10.8130081300813009No Hit
ATCACAGGCACAACTTTGATACTCAAGGTCCGTATCATTAACCCTAGCTC10.8130081300813009No Hit
GATTTTGCCTAAACTACCGGCCGACTCAATAATATAATTGTGCATATGGG10.8130081300813009No Hit
CTCTTGTAGTAACTATAAACATGAAAAATTGGAGTTCAAGGTTTAATGTT10.8130081300813009No Hit
CTGTTGTGTCGGTCACCACCCATTGGCTCCGCGTTCGGCTCCCGATGGGG10.8130081300813009No Hit
GTAGAAAATCCTGAAAAATGATTACGTTTATTGGGTAGAAGAGACAGAGA10.8130081300813009No Hit
ATTTATACTAGATCAGTACCCACGCTACGCGTGTGTGTGTGTAGAAACTT10.8130081300813009No Hit
AATCTAGATAATACCCAAATCCAACAATTACAAATAATAATTTATAACAA10.8130081300813009No Hit
CAATGAAATTGTAGACAATCTCTTTTAATCAACACTCAGCAAACTAAATT10.8130081300813009No Hit
ATCTTTAGATATTAATTAAAGTGTCAAACGAATTGAGTAGCCTGATAGTT10.8130081300813009No Hit
CCTTTGTCCCAGCCTTCGTATTTTGGGGAACGGTACAGAATTTGTACAGG10.8130081300813009No Hit
ACGGTTCTCACGGTCCTTTACAGAATTTAATGCCAAAGACGGGTTTTACC10.8130081300813009No Hit
TTTGTGTTCTGTGTGTGTCTCTCTCTTGTCCGTCTTGCGGTCTGAGGTCG10.8130081300813009No Hit
ACTTAACACTTAAAAACTCAATTATCGTCAGAAATGCCATTTAAATTACT10.8130081300813009No Hit
GATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGTAGCT10.8130081300813009No Hit
GTTTAAATGTTTCTATGTTATGTATGCATGTATATATTAATAAACATATC10.8130081300813009No Hit
GATTTCTCCCTTCTCTAGTGTCTAGTACGGGGCAAGTGGGCGGTGGCTCT10.8130081300813009No Hit
AAACACTACCAACCCGAAGTCTGGAGAAACCGATTCCAAACCTGACAGCT10.8130081300813009No Hit
GTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGC10.8130081300813009No Hit
GATTAACCATAAACCCAATTAATCTTATATTATCTAATTAGAAATACTCC10.8130081300813009No Hit
AAATACAAGAATACACAGTATTTTGTTTATGGCACGATCCACTCAAACCA10.8130081300813009No Hit
TAGCAATAAAATGCGATTCCCTTACACCTAGGATGACCAGAATGNGTTGA10.8130081300813009No Hit
CAGGTGTTTCCGTGAGACGATCAGGTGGAGATACGCAATTCCAACCTTCC10.8130081300813009No Hit
CTTTCAATTTACTTGACAGGTTCAAGGCTTCTCATTCATTCAAGTTCCGG10.8130081300813009No Hit
ATACTCTAGTGTTAATTATTGTTTACCTTTGGACGAGCCATTAGAGCGAT10.8130081300813009No Hit
CAGATATATCCAACCAATTAGAAAGATATGCAAACACAAGTGCAGTTGTT10.8130081300813009No Hit
GTGTTTGGATGCCGTTGCGGGGTGTTGCCGCGCGGGGTTGGGATTCGGCG10.8130081300813009No Hit
GTACCACCTCTTCGGTTAGGTGGGGTAAAGGTGTGTTCGGTATTGCGAAA10.8130081300813009No Hit
CTACATAACAGCCGGATAGACCGACCGCCTGTGGGGGTTTGTTGGTGGGA10.8130081300813009No Hit
ATCACACATCTTCTTAGCTCTGCTATGAAAACTATTTAGTGTTTTCCTTC10.8130081300813009No Hit
ATCTATGATTGCGGATGGACGACATAAATAATTAACCGAAGTTGCCCGAG10.8130081300813009No Hit
GATAAGAATTTCATTTGGTTTATTATATCGAAGCAAAAACAAATTAAATT10.8130081300813009No Hit
ACTAGTCCCTAATCAAAGACATTTAAGTAAATTCATCTGTATTTAGAAAA10.8130081300813009No Hit
TGTATTCACCTTCAATTCAGCGAACCTCGTCACCAGTACCTCGCACACCT10.8130081300813009No Hit
CCTGTTGACATCTCAGTCTCGTTTCTAGAGATCCNCTCTCTTTTCTTTCT10.8130081300813009No Hit
AAATTTCACTATACTTGGACTCGGTTTGGCATATTGAACGTGTGTGCACA10.8130081300813009No Hit
ATGATTCATCTGCGATTGAAGATGTACTTACTCGAAATGGTGCGTTGTTA10.8130081300813009No Hit
CACCATCCGCTCTCCCTTCTTGCCCCCATAGCCAAAAACCGTCATAACCG10.8130081300813009No Hit
GATCTAGGGCCCTGATGTGAGTGCGCGCATGAGGCTCGCGTGCGGTGGGC10.8130081300813009No Hit
AAGTTAAAATGTCACAATAAACGACATGTTCTCCAAATAGGGTAATAGGT10.8130081300813009No Hit
GTGTGAAGCTGTGTCAAACCTAAATACCCAAGAAGCATTAGCTCTCCCTG10.8130081300813009No Hit
ACAAATCACCAGAAAACACCCCGCACCCCCACCACGCACTAGCACCCTAA10.8130081300813009No Hit
GCTATTATCCTTTTGTAAGACCACCGTACCTTTTCATTCCATTAATGTAG10.8130081300813009No Hit
TTATAAGCCTAAGTAGTCGTTCCTTGTTAGAAGACACAAAGCCAAAGACT10.8130081300813009No Hit
CGGCAGGCCTGTTCCATTGTATTCAAACTCTCAAGAGGACACTCTAATCC10.8130081300813009No Hit
ATCTAGAAACATACACGTTCACAGACACAACAAACACAATGCACTCAAAC10.8130081300813009No Hit
GCAAAGAGGACTTAACTGAACTTTTGTGATCATGAGATTAAACATTATGC10.8130081300813009No Hit
ATGCTATAACTGTAGAATTGTTCCGATCCAAATGTTCAAAAGATAGGTTT10.8130081300813009No Hit
ATGCTACACATCAGGACAAAACTAGCCCGAGTCTATATGGTGGTGGTCCT10.8130081300813009No Hit
ATTGATAACATTCGATTTGTTGTTTGGGTATTGTGGTGTTTTGGTGTGAA10.8130081300813009No Hit
GAGGGACTAACAATGGTGCCTCACGAGTAAGCGGTTCCGGTAGGGTAGCG10.8130081300813009No Hit
GTTCAAACCTCACCCCAAGTAAACTCATCCCATTAACCGACTAGAACACA10.8130081300813009No Hit
TCATAATATCTTACCATTCACCATAAAAGCGGACGAGTGGCCACAGTCCT10.8130081300813009No Hit
CCAAAGAAGTCTTTTCAAATCTTCTTAAATTTTTCAAAATTAAGAAGAAT10.8130081300813009No Hit
CTAATGCTCTGACCCGAGTAGCATGGCGAACGTGCAATCCCGTGTGAATC10.8130081300813009No Hit
ATGAATCAAACATAACAAATATACGTTCTATCTCTAAAATAGATAAATCT10.8130081300813009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)