FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_chi_028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_chi_028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACATACCTCACTTTTATTCAGACCAAGTTGTTGAGATGTCTTACAAAA16.25No Hit
GCCGTGCTCAGGCCAGCATCGGTTTTGGCGGCTGGATAAAGGCCCCAGGA16.25No Hit
GTGCCGTTTCATTAAACTATATTAGACCAAGTTTTTTATTCAGAGAATAT16.25No Hit
GAGCTCATCGCAACCAACCTTCTTGTTATCCTCATGATGTCAAAATAAGT16.25No Hit
AACTTACACAAATAAATACAAAAACAGTTCAAAATAAACAAACAAAAAAA16.25No Hit
ATTTTATCTCTTAGTTGACAATGTTGTTTGATTAAAACGTATATACATAA16.25No Hit
AAATTACACATTCCAACTTAATCTTCAAAAATAAAAACACTACATAAACT16.25No Hit
GTACTAAGTTTCTTCTATTTTGCTGCATCGCTTTCGTCTTGGTCACAATC16.25No Hit
GCCTAAGTAGTGTTTCCTTGTTAGAACATACAAAGCCAAAGACTCATACG16.25No Hit
GATATCATCATCTACATCCAGAAAGCCGTATGCTTTGGAAGAAGCTTGTA16.25No Hit
GTGTATTACTTGTTTCTTTGCTTTTTGATTCTTCTGCTTAGTGCATTGAT16.25No Hit
ATTTAAAAATCAGTAATGTCAATATAATTAAAGGATAGATTAGAAAAAAT16.25No Hit
CATACACATGACATCAAGTCATATTTGGCTCCAAAACACTAACCAACCGT16.25No Hit
CCTCTCAGTCTCAGCAAATGGTAAGTTGGCTTAAAGGGTGTTTTGTGGTT16.25No Hit
AAAATGTAGTATCAATTTCTAAGCTCAATTGTAACTTTCAGTATTATAAT16.25No Hit
ACTATGAAAACNGGGCAAGAAGAGGCAGACGACGGTGCGCAATGAGACTG16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)