FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAGATAAAAAAAAAATCTAAAACTCATGGAGATGAGACAAAAATGTG23.8461538461538463No Hit
AAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTA23.8461538461538463No Hit
GCTCTTACGAAAGAAGACAGAAGTGTCGAAAATTCAATCTTAAATTAAAT11.9230769230769231No Hit
GCTTAAAAATCTGCAAATTTTTAACAAACTTGAGAATCATATACAGTATA11.9230769230769231No Hit
CAATATAATTTAGATAAATGTAAAACTGTTGTAAACAAAAAGTACACAAC11.9230769230769231No Hit
CAAGGATTGTTTGAATAATTTATGCTTGGTACTTTAGAGATGTGAAGAGA11.9230769230769231No Hit
AAATGAATGTCTCTTTGCTTCTTCTTCATCATACTTCAACCACTCTTCTT11.9230769230769231No Hit
ACAATAAGTGGGGAAGAACATAAGTTTGTTTGAGAGACACTGTTCTTTAT11.9230769230769231No Hit
ATCACACTGTCGTGTGGGAATCATCTGAGATCATGCTCCATTTGATCAGA11.9230769230769231No Hit
GTTTCGGATGATCTCTTCATACAAAACAGCAAAAGAAGTTTGGGAGGCGC11.9230769230769231No Hit
ATTTGATGTGATATTCTTGAAGATTTGGAGTGTTTCGAATCAAGTAACAA11.9230769230769231No Hit
GGAGTATGCTAATGAAAGTTGACCTCTGTCACTGGCGATTGAGTTAGTTT11.9230769230769231No Hit
AGAGAATTGCATTTTAATAACAAAATTAATTTGTGTTATTTTAAAAAATT11.9230769230769231No Hit
CGTTGTAGGAAGTCTGATGTATGCAATGGTTTGTACAAGGCCAGATATTG11.9230769230769231No Hit
GACTTTGATGAGTATAATAAACCCAAACCAATTGTTCCTTTGAGGTGTTG11.9230769230769231No Hit
CTTATTAGGTTCCGCAGTTATAGCGATTCGCAATCCACAAACCATTCCAA11.9230769230769231No Hit
ACATAATATATGTATTGGTTTTAGAGTCTTTTCTATATATTGAGTCTTAT11.9230769230769231No Hit
CCCATAATCTTTGTAGTTAGTTAAAGAAATCAAAAAAACCTTTGAATTGA11.9230769230769231No Hit
GAAAATGTGAAGCGTAAAACAATCTGGAGAATGAAACAAAACAGAAAACA11.9230769230769231No Hit
GTAAATATCTAAAACAACCAAACCCACTGTCGTGGTGGATGATCTCGATT11.9230769230769231No Hit
TGTAGATTGTTACAAAGGTCAAGAACTGTGCTGTATTTGTGAAAAATGGT11.9230769230769231No Hit
ATCATATACTATGCACCCACGAATATNTATCTTAGTAANAAATNTTAGAT11.9230769230769231No Hit
CTTCCCGCTGCTTTTACTTTACACCCAAAAAAAAATAAAAAATTGGTGAA11.9230769230769231No Hit
CCATAGGGCAATGCAGCATCTCATTCATTGTTAGAGTCTCAACCAGTAGT11.9230769230769231No Hit
GTTTAGCATCTTGATTACCTGCAAAAATAGAGTAATATAGGTTGTTAGAC11.9230769230769231No Hit
AATTTGTTTTGAGCAACAGGACCGTGTGAGTGGTGCCAAACTTGCGACGT11.9230769230769231No Hit
TCTTCGACTCACTTTCATTCAGTGGCTTTGTGGCCTTCTTCGCAAACATC11.9230769230769231No Hit
AAAGAGAATGGCTCGGCTATACTATCCAGCCGAGCCAGAAAAAATTAGAT11.9230769230769231No Hit
CAAGGATTGTTAGAATACGTTATGCTTGGTCCTTGAGAGATGTGAAGAGA11.9230769230769231No Hit
GCGGGGGGCGGGGGGGTGGGGGGGGGGGGGGGGGGTGGTGGTGGGTGGTG11.9230769230769231No Hit
CAAGGATTGTTTGAATAATTTATGCTTGGTACTTAAGAGATGTGAAGAGA11.9230769230769231No Hit
GTTCAGAGTTGTTCCTTTATTCATATAATATGGCCCTCCCTGAGACACAA11.9230769230769231No Hit
CGCCAGGATCGGGAATGAAACTCGCCCCCTTCAACGTCAGCGCTTCTGCG11.9230769230769231No Hit
GTGTCAATTATGGCTCTTCCCCCGTGGCTCGAGTCCCTATATGCAAACGA11.9230769230769231No Hit
GGGGGGGGGGGGCGCGGGGGGGGGGGGGGGGGGGGCGCGGGGGGCGGCGG11.9230769230769231No Hit
GGCGTGGGGGGGGTGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.9230769230769231No Hit
GATAATATTTGTTCAAAGCTAGACGAGATACACTCTTTGGTTTGGGATCA11.9230769230769231No Hit
GTTTTATCGGTTTATTGATCAGGGTTTAATAGAAGAACTTTCTAAGTTTC11.9230769230769231No Hit
CTTCGGTTACGACAGAGGGTTCGAGAACAGAGCCTTTTTTAAATTTGGGC11.9230769230769231No Hit
TTATTTTGTTGTTTTGGTCGTATATATAAATTTAGATCTTACGTATGTGT11.9230769230769231No Hit
GATATATTTTTAGAATTGTAACAAATTTAAACAATATATATATACAGACA11.9230769230769231No Hit
ATATATGTATAAACCTGAGCAAACTTAAACTCATTCCGCAATCGACTGCA11.9230769230769231No Hit
GTCTGGTTTGTCTTGGCTGTGCGTGATGAGCTTGGCTCGTTGGCGGGCGG11.9230769230769231No Hit
CCATAGTCAACTCAAACTAATATAGATGAAGTACATATAATAGNAAGATT11.9230769230769231No Hit
CTCTTCGAGAGTACAGCTCAGTTTGTGGTACCAAGCTCTTGGAGATTGCT11.9230769230769231No Hit
GTATAATCATAAATTTCAAGCTCTGCACTTTCAATCCAACAAAAACAAAT11.9230769230769231No Hit
GTATGGCCGTGCCTAAGGGCATATCGGTTGAAGTAGATTCTTCTTTTTGA11.9230769230769231No Hit
ATATTCCACCTCATCACCGTCACCACCACCTCCATCGTCTTCTCCTGCTA11.9230769230769231No Hit
CGACTATATGGTACTTTGGTTCTTCGACAGTCGTCCTTAGGCTTCCATCT11.9230769230769231No Hit
GGGGGTTGGAATCGACACATTGATTTTTTCACAATTTTTGTTGAACCGAA11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)