FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTATGTATACAAAAAAAAAAAGTATGAATATATATTCTTTTT22.7027027027027026No Hit
GAAACATTCTTTACTTTATTTAGAAACTCATATCTATATGAAAAAAAATT11.3513513513513513No Hit
ATCGATGAAATGGAAGAAATCCGAGTGAATGGAAAAGACAAAATTAATAA11.3513513513513513No Hit
AGGGGGCGGGGGCGCGGCTCGTTCGGGGGCGCGCGATGCGATAGGGTATA11.3513513513513513No Hit
GTTATGAGAGCNTGAAAGAAAAAAAAAAAATATATAAATATATATATATA11.3513513513513513No Hit
GTCTAGCCCATATTGGGNTGAATTATACAACCAAACCGGCTTATACGTTC11.3513513513513513No Hit
GGAAACATTCACAAAGAAACCCAAAACAGAGAGTTACAACCAACCCACCC11.3513513513513513No Hit
GTGTTGAAGATTTCAATTCAATAATTATAAAACTAAAGAATGAGATACAA11.3513513513513513No Hit
CCAATCCAGTAGTCGAAGAAGAGATTGATTGGAGAATACATTTCCTTGAA11.3513513513513513No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGTGGGGGGCGGGGGGGGGGGGGGGG11.3513513513513513No Hit
GTATTATATATATTTCCACTCAAAAACAAATCTTGTTAGGGTAAAAGATC11.3513513513513513No Hit
GTGTGCATCCGGTACCGATTTATGGAAAAGATTGGTCTGGTGTTTCGGGT11.3513513513513513No Hit
AGGTGTGGGTGGGCGGGGGGGGGCGGGGGGGGGGGGTGTGGGTGGGGGGC11.3513513513513513No Hit
TTGGTGGGGGGGTGGTGGGTGGGGGGGGGGGGGGGGGTTGGTGGGGGGGT11.3513513513513513No Hit
ATGTTTAAATGCCGGAAACCCTATTGCTGAATACCGTGAAACCCTATTGG11.3513513513513513No Hit
GGCATGTTCCACGTAATATGCCGACGGGGAACATCATCATTGGCACAAGA11.3513513513513513No Hit
GGCCTATGATTCAGGATGCTCAGAGAAAAAACAATGGTTTCACTAGGGAC11.3513513513513513No Hit
TTCAAGGAGTGCCATGAGAAAAAGGACAAAAAGTTTAAAAAAAGTAAAAG11.3513513513513513No Hit
TTTGTGTTCTGTTAGAATTATTTTTGTGCTTATTGCTTTTCATTTTGGCT11.3513513513513513No Hit
ATCTAATGCTGTCTCGTATATGATCTTTCGATAAACGAACAAGGAAGACA11.3513513513513513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3513513513513513No Hit
CCAAGAATTCTGATTACCTCCATAACTATTCGTCTGACCATTACCACTGA11.3513513513513513No Hit
TATTCATATCTTAAAATTTAGTATTTGATATTTGCTTTAGTTTTAACAAA11.3513513513513513No Hit
GGGGGTGGGGGGTGCCGAGGGGGAGTGGGCTGGGCTTGGCGCGATCTCGG11.3513513513513513No Hit
AACTTAGAGAATATATTTTATATATTGGAGTTAGATTTAAATGATGAGCC11.3513513513513513No Hit
GGAATCAGATTTGGGGGTTCGAATGTGTTGGGGTGATGGGGTGTGGGTGG11.3513513513513513No Hit
TTCTTGCTTGGTGTGGGTGATGAGGCGGGGGTGTTGAGTGCTCCTGCGGG11.3513513513513513No Hit
GTTTCAGTGATGGACGATCTGATAGAGGGACTAAGTTTCTTGAACCAGGA11.3513513513513513No Hit
GTAGTAAATAAGCTTATCTGTTTGTACGGATTTTAATGCTTCTCTTCAAC11.3513513513513513No Hit
GATGTGAACGGGGGGGTGGTGTGGGGGGGGGGCTGGGGGGGAGGCTGTGG11.3513513513513513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG11.3513513513513513No Hit
AGGGGGGTGGGGGGGGGGGGGGGGTGGGGGGGGGGGGTGGGGGGGGGGGG11.3513513513513513No Hit
TCCAAACACCATTCTTTGAGCATCTGTGTGCTGACCATCATCCCACGGAA11.3513513513513513No Hit
ATTTATTTCCTAGCACAAATAAAATCATCCATATATATTAAAATATCCCT11.3513513513513513No Hit
AAGGTTGTTTTGTTTAGGGGTTTGTAATTTGCTGTGTTTTCTTGCGAATT11.3513513513513513No Hit
AGTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATATTGACTTTGG11.3513513513513513No Hit
CTTGCTCATATCTAATTTTCTATTCGACAGTTTTTTTTTTGAGAAAGAAA11.3513513513513513No Hit
CTAGGTCTTCCCGTTCCGTCATCTGGCTTATGTTCTTCATGTAGCATTCA11.3513513513513513No Hit
TTACTGAAGAGTATATACTTCCACGATACATATTTATGTGAAGCAACAGT11.3513513513513513No Hit
ATACNCTTCATGTTGCTCCAAGTAACATNGAAAACAAGAATTCTCGTCAG11.3513513513513513No Hit
ATATGGGGGGGTTGTGGGTTGGTTGTGAGGGGGGGTTGTGTGGGGTGGGG11.3513513513513513No Hit
CTGATAAACACCAGAATAAGCTATATTCACCGGGCCAAGAGCACCTCCTC11.3513513513513513No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3513513513513513No Hit
GGTAATTACATATGATTTAATCTAACATTAGTCTGATAATAACAGCTCCA11.3513513513513513No Hit
GGTAAATATTGTATAATTGCTCGGTTTTCTGGAATTTTTTCCATTCCTCT11.3513513513513513No Hit
AAATCCCGGCTCAACGTGGACAAGCGTTTCAAGTTCCAAGACAGCCACAC11.3513513513513513No Hit
GTCTGCAACTTATCTAATTGTACCATGAAAAAATTAAGCTTTTTCAGCCG11.3513513513513513No Hit
GGTGGGGTCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3513513513513513No Hit
CGCGGGGGGGGTGGGGGGGGGGGGGGCCGGCGGGGGGGGGGCGGGCGGGG11.3513513513513513No Hit
CAACACATGACTAGTTACGTTGAACTATCATCACTAGCTAGACACTAGTT11.3513513513513513No Hit
GTGCCCCGGGCGGGCGGGTGGGGGGGGGTGGCTGGGTGGGGGGGGGTGGC11.3513513513513513No Hit
ATTTAGTCCCCGAGCTTTCTTTCACGATCATCCTTAAAAAGCGCTTGCTT11.3513513513513513No Hit
CTATTTCTTAGATTCATGGACCCAATTCAATTCAGTGGGATCTTTCATTC11.3513513513513513No Hit
TGCAATGCCATTGCAAAAGCAAATCGGATGGGCTATATTACAGAAATGCA11.3513513513513513No Hit
GCGTTGTTGGGTGTCGGTTCTGGATGCATGCGGCTTTGACGGGCGGGTGG11.3513513513513513No Hit
GCCAAAAATAAGTGTTATAAAGATTATTGTGACCAGATTGGAAGAAGAAG11.3513513513513513No Hit
ATATTACATTGTTTCCTCCGGTCCTTGTGAGAACTCATCTAAATTTTTTT11.3513513513513513No Hit
GGGTTGCGGATCTAGCTTTGGTGAAGAAAGGTTGTAAGAAAGCAGCTCAG11.3513513513513513No Hit
ACACAACATTAAGAATAAAGCCCGCACCTGTCCATTTGTTCCAAAGCCAT11.3513513513513513No Hit
GTTTACAATGGNTCTCCAAAGCACTGAGGTGATTACAAATTTATTTTACA11.3513513513513513No Hit
GAGCAGTAAGGGGTCAAGATTTCTAAAGCCTGAATTTGTGACTTTTTTTG11.3513513513513513No Hit
GATCTGGTAATAGCGTCGGGAGGGTGCCGGGGGGGAGGGCGGGCGCGGGG11.3513513513513513No Hit
GTATAGGAAAGAGTGAAAAAGTGCTTTCGTTGTTGTGGAATAAGAAGCCT11.3513513513513513No Hit
ATGTCGTAGGTNGGGTTGGGGGGGAGGGGTGGGGGGGGGGGGGGGGGCCG11.3513513513513513No Hit
ATCTTCATATTATCAATCTATGGNATATCGTATAATGACTAATCTAATCA11.3513513513513513No Hit
AGGCTCGGGTGTGGGGGCTGTGGGAGCGGGGTCTGGGGGCTGGTATGGGC11.3513513513513513No Hit
ATATTACCTCGTGTTGTGTCGATGCTGTATGAGCGTTATAGCTTGGAAGT11.3513513513513513No Hit
CATCATAAAAGNACTAATTAAAAGGAACCAATCAAAACTACGCCAGACTC11.3513513513513513No Hit
GTTGAATGTAGAAATGTATATTTGGACGTCAGATTCTATAATATATAATT11.3513513513513513No Hit
GTGTCCTCCGCCGGGGGGGCCAGCTGCTGGCCGGCCGGGGGGGGGGGCGC11.3513513513513513No Hit
CCAATGGACAGGCCACCAAATATTCAAGTTTTCATCAACTTCCGCGGAGA11.3513513513513513No Hit
GATTAATAACACGTACCTTTTCCAAGCTGTTCACAAATCTTCTCAAAAGT11.3513513513513513No Hit
ATTCTATTTTTCTCCTCTCTTTTTATTTTTCTTCCTATTTTATCTTTTTC11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)