FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGCTTGCTCGGCCAAGTCGCCGAGCATTTGCAGGCCGCCAAGTCC215.384615384615385No Hit
ATCTTTCCACTATCAGGTATAAGCCAAATGCTGGCCCATGGCCATGAAGA17.6923076923076925No Hit
GGATTTCTACCGGCAAACGATAGAGCCGACCCCGAAGGTGCACCAGTCAG17.6923076923076925No Hit
AGATAAGACTATAGCGACTTTCTCTTCTTGCTAGAACTATGTATATCCAA17.6923076923076925No Hit
ACCTATCACTAAAATACTCCTAGAGGGGGATAGGGCTAAGCGGAGCGAAA17.6923076923076925No Hit
GANTGCCATATTCCCTTCCTGCAATTTTTTCAGGTTGTCATTGAGGAATA17.6923076923076925No Hit
TATCGGGTATGATCGAGAACTATGGGAGTCAGTCGTCGTGCAACCTGACA17.6923076923076925No Hit
ACATATGCATGTGCGCATTGATAATCTTTTTCATTTAACCAAAATTCAGT17.6923076923076925No Hit
ATAAGCCTACATGTGATGTGGTATACATCCATGGTGTTTTGTATTATCTA17.6923076923076925No Hit
GAGTAGGATTTTCTTTTTATGCTCACAAAACTGTTTTGCGCAGTTTTGGT17.6923076923076925No Hit
CGTGGGCGCCGAGCACGCCGATCACCTGGCCGTCGGCATTGTGAATCGGC17.6923076923076925No Hit
ACTTACAATAGAAATTATAGCATTATCTTTTTCTTTTCTTTAAGCATTAT17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)