FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGTTTTCTATTGACACAATTAAGTTCTTTTTGAACATTCATCTGTA36.122448979591836No Hit
TTGTAAAAGTAAATGCGATGAATGTATTTTACCTCCACCAGATTCAAACC36.122448979591836No Hit
CAAACGGAAGATTCAAACTGAGGTTTTAAACTGCTGTTTGGAGACGTTTC36.122448979591836No Hit
ATATATTGGGTCATGTGTCAAGTCTTGGAGATTTTCGCCTTGATGATAGT36.122448979591836No Hit
GAGGCTTGGTTCGCTCAAGCCGCTGAGTATTGGAAACAGGCTATAACGCT24.081632653061225No Hit
AGTCAAAACATATCACTTTTTTTGAATGAATATGTAAAAATTGGAAAATA24.081632653061225No Hit
GTCCCCTAGAGAACCACACACAAACAATACAGCTTTGAATATCTCCTTGT12.0408163265306123No Hit
AGTTAGGGCTATACGGACTCGAACCGTAGACCTGCTCGGTAAAAGAGTTC12.0408163265306123No Hit
GGATCAAGTCAAAATAGAAAAACTCACATTCATTGGGTTTAGGGATAATC12.0408163265306123No Hit
GGTGTATATATATAATTTTGTTATTCATTTTCATATATATCAATCTTATT12.0408163265306123No Hit
GCGCACCCGCTATCTGGTGATCTACGCACTGCTGATGAGCGCGGCGTTCT12.0408163265306123No Hit
ATGATAGGCATTATTTTTTTTTCTTCCAAAAAAGAATTAGGACAATACTC12.0408163265306123No Hit
ATACTTAATATTTTAGTTTTGCTTACNATCTATTTAATTCTTTAATTATC12.0408163265306123No Hit
AAGTAGTATCGGATACCACTCATACTTGGAACTGGAGAGGTCCAAACATC12.0408163265306123No Hit
GGTTATGACACAAGCCTTCTCTCCAAATTGCCAGTCNGTTGTGTGTGGTG12.0408163265306123No Hit
TTTATGCCGTTAGCTCGAGGGACTGCAATAACGGGATTTGTAGTGTTACT12.0408163265306123No Hit
GCCACGTGTCGTGAAAATTTGAATCTACAGAATTCTCCCCGATTACAATA12.0408163265306123No Hit
TTTGTATCCCATTACCATGTCAAACTCTCCTAGTCCAAACAACAGAAAGT12.0408163265306123No Hit
CTCTTTCTCTTGCTTGATTCTCTAAGTGTGTAAATAGAGAATGGATGGTC12.0408163265306123No Hit
TGACCGCTGGAAGTGTCCTTGTAATTCACCAGGTGAATTTTTCGAAGTTC12.0408163265306123No Hit
GTGGTAAGCCTTTTGCCAACCTTTTAGAGGCGTCACATCTCTTCAAATTT12.0408163265306123No Hit
CTTTTGCAAGATGATTGGTTTTCAGCAGATTTGGAACTGGATTCCGGGTT12.0408163265306123No Hit
GTAACGTCTAGAACATTGTGCTACTGATTTGCCTTTGTGTTGCATTATTT12.0408163265306123No Hit
AAGGCATTCCTTTCACTTAAGGTAAAGAGTATTCCGGCATTCGAAGGGAA12.0408163265306123No Hit
CACTTTGTACACCGATTTGTCTCATACACCGATTTGTTTCGTATACTGAC12.0408163265306123No Hit
ATAAANGCCAAACTAAGCAATAAAGTACCTCGTATTTTTCCTTCTGCCTC12.0408163265306123No Hit
CCCTAAGCTTGAGAACTTCCTTCTAAAGTTTCTCCACCTTCTCTCCAAGC12.0408163265306123No Hit
GTACTATATGTGATAATTTTTTAAAATTGAGACTTGGAAAATAAATAGAA12.0408163265306123No Hit
GATACTTATGANATCGATGAAATTTATTTTCAAATCTTTCTTCTTAGAAC12.0408163265306123No Hit
ATTTTTATATAGTATGCGCATATTGTTTTAACGAATTAAAGATTTTATAG12.0408163265306123No Hit
ATTAATGGTTTTGCTTTTTTCTTTTTTTTTTTTGGCTCTTGGCAGATCGG12.0408163265306123No Hit
CACCTACTCAGCCCCGACCTATCCCGATTCCCACCCCTAGTCCGTCCTCT12.0408163265306123No Hit
TAACTGAATTCACCAATAAACTTAAACATCGGACAAGTGTTTACATTTTT12.0408163265306123No Hit
ACACTAAAGTATGGGCTAGTGATAGATTTTATTCTGTCCCCTGTAGGAGA12.0408163265306123No Hit
CTTTTATACTAGGTAATCTAGTATCCTTATGCATGAAGATAATCAATTCG12.0408163265306123No Hit
AAACATAATATCCGGTAAAGTGAAAGTAAGAAGGTAAAGAGATAAGAAAG12.0408163265306123No Hit
CATTAAGGCTTCGTTGATCAAAAGGCTTGGAGTGAAGCCTAAGGAAGGTC12.0408163265306123No Hit
CTCCGGCTCCGGCAAGTCGAGCCTCCTGCGCTGCATCAATGGCCTCAACA12.0408163265306123No Hit
GTATTTCAACTCAAGAGGAAGATTGTAAAAACCATGAGGGTGGGTTCCTA12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)