FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACTTCTCCGTATTGGTTATTTTAGATTTGTGTACTTAGGCCACAGATA23.0303030303030303No Hit
GAGAATTCTCACACACTTACTTGTTCTCATGATGAACCATTTAAACTGTC23.0303030303030303No Hit
CTAGAATTCCATCTGTTTTGTGGGTTTGGTATAGTACGGATTTTCATGTA11.5151515151515151No Hit
ATGTATGGCGGTTAAGATGCTCGTGTTAGAGTAACCAGCATAGGCGCAAG11.5151515151515151No Hit
TACTTAAACGTGTAGGCTCGACGGGGGATATATACAAAGTATAACAGTGG11.5151515151515151No Hit
ATTGTTTCGGGGGGGGGGGTGGAGTGGGGGTGGGGGGGGGGTGGGGGGGG11.5151515151515151No Hit
GGATATATACCATGCTCGGGGAAGTAGGTGATTTAGAAGTTCGACCAAGT11.5151515151515151No Hit
ATTTGATTATAATTCAATTCGTGACGATCATAAGTTGAAAATTCATATTC11.5151515151515151No Hit
ATTCCACCAAGATCTCTTCGTTAGTTGGGGGAAGAATCCGCACGAATCGG11.5151515151515151No Hit
ATCCATTAGATCATGAACTTTTGGGTCTCAGCCCTCTCGGGCGCTGCGTC11.5151515151515151No Hit
ATTATAAACCCATACGGTGACGTACCACAGAATCCTTTTTGGCCGAGCCG11.5151515151515151No Hit
GGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.5151515151515151No Hit
AGCTAGCTCTGGGTTCGAGTGGCATTTCACCCCTAACCACAACTCATCCG11.5151515151515151No Hit
CCCCTTAAATTTATTATATACAAAATAGTTTGTGTGTTCTATCTCAATTT11.5151515151515151No Hit
CTTTAGGCTGGCGTTGGTGGAAGGACGGGGCGGGGGGGGGGGCGGGGCGG11.5151515151515151No Hit
AGCTGAGATTACATAATATAGTTTACACACAACTCTATTTTTTTTTTCTT11.5151515151515151No Hit
CTACCTATACAAGTCAAATTAAATATATATAACATTTATCGTGAATTACT11.5151515151515151No Hit
GGTCGGTCGCAGGGGGTGTGGGGTGGGGGTGGGGTGGGGGGGGGGGGGGG11.5151515151515151No Hit
TCCCGCAGTGATATAAAATCAAAGAAAATGGGTGCTACGAAACAACACTG11.5151515151515151No Hit
TTTTACCCATCGACCATCAAGCCTTTATTGGGAAATTTGATATGGATGTG11.5151515151515151No Hit
AGAACAGCATATAAGTTATCATGCTCGCATATCCACCATTTGAGTCTCCA11.5151515151515151No Hit
ATAGAAAGAAAGGTAGATATATATATATATATTACCTCAGATGATATCAA11.5151515151515151No Hit
TCTACGTCTAGAGGATCTGCGAATCCCTCCTGCTTATACTAAAACTTTCC11.5151515151515151No Hit
CATGCTTGGCCGTGCAGGCAAGCTAGAGGAAGCATTTGAGTTAGCAATAT11.5151515151515151No Hit
GAATTATTGTGTTTTTGTCTTACTGCACGGTGTTTGGTTTTGAGTAGGGT11.5151515151515151No Hit
GGAGCTGCTTGCGTAGGTGGCTGGCGCAGTGGGGGGGGGGGGGGCGGGTG11.5151515151515151No Hit
TAGTGGAGAACGGATGTGGGAAATTCTCAAAATCGCCAAAAGAGATATCA11.5151515151515151No Hit
CATGTACACATTTCTAAAACTACCTCTTTCATGTGGTATTTTCAAAACTA11.5151515151515151No Hit
ATCACTCTTGAGGTGCTGATATCATATTTTCCTAAACTCTACAAGAAAAG11.5151515151515151No Hit
ATTAGGTCTTGTGGGTTTGAATTCAATTAAGATCTTTTTTTATCTGAATT11.5151515151515151No Hit
CTCAAGGAGAAGTTAACTACGAAGCCGTTCCAAAATGGCCATGGCTCGTG11.5151515151515151No Hit
ATTGTGGTGAAATTGGGCTTTAAGCTATACAGCGCGGTATTGGTTGAGCT11.5151515151515151No Hit
TATATATATTTGATAAAAGGGACAAGATTCAAATAAATAATAAATAGTAA11.5151515151515151No Hit
GTAGTCACCTTGTTAGATCTAGGGTTTCAAAAGGGATTATGAAAATTGAA11.5151515151515151No Hit
AGGGGGGGCCTGGGTTGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGG11.5151515151515151No Hit
CATAAGCGCTGGCTTAANTCTCATCACCTGGAGCAGAGATCAAGTTCCCT11.5151515151515151No Hit
CGGGTCGTGTCGGGGGGGGGTGGGGGTGGGGGGGTGGGGGGGGTGGCGGG11.5151515151515151No Hit
GGCGGGGTGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGG11.5151515151515151No Hit
ACTCTATGGTTCGGTTCGTTAGCAGGTTTATTAATAGAGATTAATCGGTT11.5151515151515151No Hit
GGGGGGCGGCGGCGGGGGGGGGGGGCGGGGCGCCGGCGCGGCAGGGGGGG11.5151515151515151No Hit
CTATTAACTTTTTGAAGTTTGCTGGTTTGAAACTGATGCTGGTAACTTAC11.5151515151515151No Hit
GTGGGGGTGGGGGGGGTGTGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG11.5151515151515151No Hit
GTAACAGGAAAAGATCTTATGATAGTCAATATGGGACATAACCACCCATC11.5151515151515151No Hit
ACGCGGGGGGGGGGCGTTGGGGCGGTGGGGGGGGGGGGGGGGGGGGGGGG11.5151515151515151No Hit
GTAATGGGGGGGCAGGGGGGGAGGGTGGGTGTGGCGCGGGGGGGCGGCGG11.5151515151515151No Hit
CACTTAACATCATCTCCTTTGATTTATTTTCTTTAATTTTCACGTTGAAN11.5151515151515151No Hit
GTTTTATCCAACCGTGTATTAAATTACACTGTCGATAGATTGATGAGTGG11.5151515151515151No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGG11.5151515151515151No Hit
AGATATTTGAAACCAATGAAGAGCAGGAACTGTTGGGAGTCAAAAACTTA11.5151515151515151No Hit
CAAAATAAATGAAGAGGATAAAAAGTTTTTTCATTAATATGAACATAACG11.5151515151515151No Hit
TCTTTACTTTATAGAATCATCTAAGTTTTGGTTTTAAGTAAGTACTTAGT11.5151515151515151No Hit
ACACTATGGAGTAAGAGATGTATGGATGTTATGCAAAACTACACACACCC11.5151515151515151No Hit
CTCTTTAGGATCGATCTATCCAGGAGGTCTCCAAAATCCAATGATGTGTA11.5151515151515151No Hit
GGGGGGGGGGGGGGGGGGGGCGGGTGGGGGGGGGGGGGGGGGGGGGGGGG11.5151515151515151No Hit
AAATCAGGGCGNGGTGCAGANCATCNTCTAAAGTTCCTGGTTTATTGGAG11.5151515151515151No Hit
AGACGCGGGGGCGGGGGGGGCGGGGCCGCGGGGTGGTGTCAGGGTGGCCG11.5151515151515151No Hit
GTATTTGTCAGCATGACAATGAAGGTACGCGTCATAGCGAAGAAATTACA11.5151515151515151No Hit
CGGTGGGGGGAGTGTTGGCAGGAGGTCGCGATGGTGCATTAGTCCTATGT11.5151515151515151No Hit
ATGTTGATATAAAAGATGCGTGAAAATATTATGTTGATACATCTTTTTTG11.5151515151515151No Hit
AATCCATTGTATTTTGCAGTCACTTTTGTTAAGCGAATGACTGGTTCAGT11.5151515151515151No Hit
CGTGAAAGAAATTCTCCATGCTTACTCTTCTTCTCAGACGTCTCAATGTC11.5151515151515151No Hit
TGAATCATAGTTCGAATACTTTGTGATCTATTTGATCTATTTCGTGCTCC11.5151515151515151No Hit
GTATAGCCGAGCCATTCTCTTTTATTTATGTTGCGAAGAAGTAAAATATG11.5151515151515151No Hit
ATTAAGAAATCTTAGCAAGTTAATCGAGAAATCATATAAAGACCTGTGGA11.5151515151515151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)