FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTATAAGAACGGTGCGTTCCGGGGTGTGAAGTGGGATAGAAGGGAT21.8691588785046727No Hit
GCTATCGTAGTGAGGGCATAGACTATTTGGTTCAGCTTCCACAGCAGGTG21.8691588785046727No Hit
GTATGATTACCTGATGAGTCCGACATTGTTTTCTTTGTTGCATTTCTCGC10.9345794392523363No Hit
GAGTGGATGGGCGGGGGCTGGTGGGGGGGGTGTTGTAGGTGATTGTTTTG10.9345794392523363No Hit
AAATTTTACACTTCATCACTTTTAAAACTCAATAAACTGTTATAAATCAT10.9345794392523363No Hit
GAACTAATAAGAATCGTAGTAATTTAATGAGTTCTAAGGATTTCGATATA10.9345794392523363No Hit
GCACGTACACTTACTTTTTTCCAAACCAATTTATTGTTTCTTCGATAAGC10.9345794392523363No Hit
AAATATAGTACGTATATGTTGGGGGTATTTGAAACGCATGCTGGTGGAGA10.9345794392523363No Hit
GTGGGGGGTGGGCGGGGGTGTGTGTCTGGGTGTGGGTGTTGGGGGGGGGT10.9345794392523363No Hit
ATGCAAATCTTTCCATAATCATCATTAAGCACCTCTAGTTCCTGAGACAA10.9345794392523363No Hit
GCATTTCTCTTCTGCATGTCCATGGAGTTTTGAAAAATCCAAACATCTCA10.9345794392523363No Hit
AGGGGGGGGGTTGGCTGGTGGGCCCCGGGGCAGGGGCCTGAGGGCGGGGC10.9345794392523363No Hit
GTTCATGTGGACATTCACTGAAGAACCAGAGGGTTTCTCCTAACGATTTC10.9345794392523363No Hit
GGATGCTTGTTTTTTACTTTTATATTTGCTCTTATGACTTGTTCTTTTCA10.9345794392523363No Hit
TAAAGAGAGTGTGCAGAGAGTGCGCAGAGAGCGATGCATCGACATTTTAA10.9345794392523363No Hit
GGTTCCTTTGTGCCCCGGTTCAAACTTTTTTTTAGTATAAAAAAGCATTT10.9345794392523363No Hit
CATGAATACAGCATCTATAGGATAACTTCTGTCTTCAAAGTTATTTGAAA10.9345794392523363No Hit
GGGTTTGATACAGGTTCACGCTGGTTGGCGCTGTATGTCGTGGTGGATCT10.9345794392523363No Hit
ATTATATGCTATAGTCAAAAATGTCCTATATACCCTCACCACATAATCAA10.9345794392523363No Hit
AGTTCGTGTAAGTCGTGAATGAGCCCCTCGAAAGCTTGATGCAAATAAAC10.9345794392523363No Hit
GGATAATACTGCTGTTGTGCCGCTGGATCAGGAGCAGTTTGCTATTGCCT10.9345794392523363No Hit
TCTTACAACAGGGGATGATCGATCACACAAAGTTTATAATAATAATATAC10.9345794392523363No Hit
GTCATAAGCACATGGTGCTGTTTAGGCTACTATTTGGTCAAAGAAATTCT10.9345794392523363No Hit
CTTCAATCCAGTCCATATGATGTCTTTTTCGGGAGCTAGGGGAAATGCAT10.9345794392523363No Hit
CAAACACACTCGACTCCTGGGATGAGAGGGCACACTCCGACTATTATCCA10.9345794392523363No Hit
GCATGGTGTAGTTACTCTAGGAAGGCTATTTGCTGTAGACCACTCTTGGT10.9345794392523363No Hit
AAATATAACAGATAAATATAGTACAGAATTTAATCAAATAAGAAATAACA10.9345794392523363No Hit
AAATAGAATAAACCATTAGAGATGGTTTTAGTATATTTAGAAGAGTGGTG10.9345794392523363No Hit
ATATTATATTATTAATATTATTAATAACTAGTAATTTTTTTTTGATTTCT10.9345794392523363No Hit
TATGATATGTCTACAGGTAGACTTCCAAGCCACTCTTTGTGCTTCAAAGC10.9345794392523363No Hit
GACGTTACGAGGGAGGAAAATCTCCAGTGCAGAGGAGATGCATGAAGCAC10.9345794392523363No Hit
GGGCCTAGATTGGGCCTATGTTTTCCATCAAAATTAACTTTAATGGATTA10.9345794392523363No Hit
GCCCATATCTTTGTAATGGACAAGGATAGTCAGTAAAGCAAATCTAATTT10.9345794392523363No Hit
AAACCTATTGGATGAACTAAAAATGGATTAGCATATGAGGCACTACACAC10.9345794392523363No Hit
CTTGTACGGTGGCTATGCTCTTACAGAAGAATCGAATTCGCTTAGCTGAT10.9345794392523363No Hit
CAAGTCTTCATATTGTAAGATCAAGGCCAGAGATGCAAGGGAAAAGAATC10.9345794392523363No Hit
TTCAAACACAACAGCGATTATATCCAACGCACACTTAGGAGTGATTTTCA10.9345794392523363No Hit
GAGAAAGAAGTATGAGTAGTTTCAGGTGGATTAGATTGTAGAAGACGAAG10.9345794392523363No Hit
GTTTTGGTAATTTCAATGCAGCTAAACATAATTCGGATCACGAAGCAGCT10.9345794392523363No Hit
GGCGTACTATGAGTGGGTCCCTATCCTCCTCTTCGTCTTCTACGTCCAGT10.9345794392523363No Hit
GAATTTGGTTATTCACCATGTACGAGGATCCCCACTAAGCATCCATGGCT10.9345794392523363No Hit
ATGATATCCACCCCTAATTTTTATTTCCTGATTTTGTGAAAAAAGGGAAA10.9345794392523363No Hit
TCTAAGATTAGTTCAGGGTATAAATAACACTCTTGTGGCTACCAATAATC10.9345794392523363No Hit
GAAAGAGAATGCTTGAGAAGAATAGGGCTGAAGATTAACAGCAGCTTTGT10.9345794392523363No Hit
AGTATGCATTATATGAACAGAAAGCAAACGACCGGGATCATTCAATACAA10.9345794392523363No Hit
TGTTTGCGTTACTCAAGCCGACATTCTCGCTTCCGCTTCGTCCACCGCCG10.9345794392523363No Hit
GATAGATAAAGAGAATGAAAATAAAGATATGGATAGAATGTTGTCTCATT10.9345794392523363No Hit
AACATGGTTTATTTATATAGTGATTGTGTAATTTTGTGTATTAGTTAACA10.9345794392523363No Hit
CAAGAAAATACAAAATGCTCACAAAATTAAGAAAGACTCCTTTATGAAAA10.9345794392523363No Hit
GTTATGGAATAAGGGAAGGACTGAAAAGGATGTGGTGCGGGGCGTATGGC10.9345794392523363No Hit
ACCCTAGGCTGTAATTTTCTACAAACCTTCGAAATTTTAAACGAGACACC10.9345794392523363No Hit
TTCATGAGTACTTCAAAGAACAANTGCCTTGGTGCTGGGTAAATCGTTAT10.9345794392523363No Hit
TATTTAAATTAAGCTAAAAAATAACAGTAACTAAATTAAAAACAAGAAAT10.9345794392523363No Hit
ATCTCTATATTCTCAAATTAACATTTTATAACAACTATTGTTTATTTAAA10.9345794392523363No Hit
TGTTAACAGGTAGTAGTTGTTTTGTGAGTTTAGTTCGTTGAAGCACTGCA10.9345794392523363No Hit
GTTTCGAACCGCTGCGCCGCCAGGTCGCGATCCGCATGCGGGACGCCGGC10.9345794392523363No Hit
GTGCTTGACATAACGCCATTTTCAAATTGTCAAAGGCTACTTGAGCTTCC10.9345794392523363No Hit
ATGTTTAATTAAATAAAACCATAAATTATATTTAAAAAAAGAAATTCAAA10.9345794392523363No Hit
GGCATATATCTTCATGTATAGTAGCCATCGGTGAGATCGCTGTCATGGGA10.9345794392523363No Hit
GAAAGAATAATCAGATCTCCTCGCCATCGCCATCGCCATGGTGTGGTGTA10.9345794392523363No Hit
GCACCCAATAATAAAGCCCAGCCCAATAAACTCCAAGACTAGTAAGTTCA10.9345794392523363No Hit
ACTTTTTCATTCTTAAATGAGAAATCCCTAGAATGACACGATTTGATGTT10.9345794392523363No Hit
TGTCTCGACTAATAAGTAAGAAAATATATGATTCAGGCAACTAGTGCTGG10.9345794392523363No Hit
AATCTGCACCTACCNGATCTCCAGAACAGCTCAGTTCAGTAAGAATNACA10.9345794392523363No Hit
TTATAGTATCAATAATTAACATGTGTGTTGGACATGTTTTTGAAGTTCAT10.9345794392523363No Hit
ACTTTGTAGGCTACGGGTTCTGTTTTTCGTGCGTTTCGCTGTCGCAGTCC10.9345794392523363No Hit
GTGTATATTAGTGGTTATTGCTCTAGCCGGTATTCCGGTTATGGAGTCCC10.9345794392523363No Hit
GATTGTCTTGTGTATACATATCCAATTGTATTCAACATATCCACTCCAAA10.9345794392523363No Hit
CATACGAGTTTACCGCCCAGACCGATAGATTTCTCGGCGGCCCTTCCGAT10.9345794392523363No Hit
ATGTTAGAGGGTATAATTTTTTTTTATCATATTAACAAACTATTTATCCT10.9345794392523363No Hit
ATAAATATATATAGATTATTTGAAAATTTTATAATTCAAATTATAAAATG10.9345794392523363No Hit
CTCATCTCACTGAGTTTAAAGACCACGAACTGGTTAAGACCAAGAGAAAC10.9345794392523363No Hit
ATTTACATACAAAACAAATCCGATATTGGGGAAAAATCAGGATACAGTTA10.9345794392523363No Hit
TTATGGGTACGTCTTTCATCCAACCGCCAGTGGAGAGCAAGCGCCCCACC10.9345794392523363No Hit
GCTATATCTATGTAATGACGACAAACATTTCACATGTTTCCATGTTTTGT10.9345794392523363No Hit
CTCTCTCTCTCTCTTGTTCTTCATTTTAGGTCTTTGTAAACCTTTTATCT10.9345794392523363No Hit
GGGTTACTTGCAAGCTTCACATGGAGCGGGTTAGAGTACCAAAAGTACCA10.9345794392523363No Hit
GATTTATCAGGATAAAATCAGAATCGCTTATGAACTGACATGTGTTTCAG10.9345794392523363No Hit
ATTCTGTTCGCGGCCACAATTACATATTGATGATGAGGATAAAGCTCCTT10.9345794392523363No Hit
GGCTTTTATAATATAGCTAGTGTTGTTTATTCTACCTGGAGTCTATAGTT10.9345794392523363No Hit
ATTATAAAGAGATAGGGATCAGATGGATGAACTAATTGACTAGGGGATCA10.9345794392523363No Hit
TCTCTGGGTGCCTAGGTATCCACCGTAAGCCTTTCCTCGTTTGAACCTCG10.9345794392523363No Hit
ATCTATAGCTAAAACTTGAAACAAACCCACACTTAACGAATGCATTAATT10.9345794392523363No Hit
CAGCGTATCTCAAACGTTTCTCCTGACAACGTTCACAGTTGAGATCGACA10.9345794392523363No Hit
GATTTTTGCTGGTTGGTTCCATTATCACAAAGCAGCTAAAAAATTGGCTT10.9345794392523363No Hit
TAGTATATTCATCTACAATAATAGGTATTCCGAATCTCGAATGAGCGGTC10.9345794392523363No Hit
GTGTTATGTGTTGCAGGTTGCTTGTAGGAGGAGGAAGGGTTGGTTGAGGG10.9345794392523363No Hit
AAAGCATTCCTCTCATTCGCTTACTTCCTAAAAGGAGACTGAGGCGCATG10.9345794392523363No Hit
CCAAATATCTAATTTTGATACCAATAAATAAAGTTATTTTTGTTCTGGGC10.9345794392523363No Hit
GTCTGGGGACATATCCCAATCATGTGGACGTAGCCACTCGATTACCAATA10.9345794392523363No Hit
CTAAAGACAAGGTCACACAGGTCAAGTCAAGAGCAAGAAGAAATAAAATA10.9345794392523363No Hit
TGTCTTCACAAGTTAAAAAAGGCAACCTAAGGAAGAAAGGACCAAAGATA10.9345794392523363No Hit
AGCTCAAGGAAGTCGTTGACTTACTTGGAGCTGAAGCTTTTGTATGGATT10.9345794392523363No Hit
CTCTAAGACTCATTTTGTTTTATAAAATAATTTATAAAAAAACGAAACCA10.9345794392523363No Hit
AGATTACNTAGNATAAAAGATTGAAAAACCATCACAAACCTCCCTTTTTT10.9345794392523363No Hit
GAATGCGGCACCAGGCTAGTAGTTTATATGGATAGCTGAAGGCGCTGAGT10.9345794392523363No Hit
AGGTTAGGCTTCTCACTAAGCTTCTCTAGTTAATGATCTCTGTGATTGAT10.9345794392523363No Hit
ATCATAAGAATTACATGAACTCATATTTAATACATAAACAATAACCAATA10.9345794392523363No Hit
GTTTAGTGCACGTAACTGGCTACGTGGATTTGAAGAGAATGCTAAAATCC10.9345794392523363No Hit
GAGTGATTATTAAAATAAATTGAAAAGGTCAATGGTAGTCAAAGTTTTAG10.9345794392523363No Hit
ACCCTACACTTTTTACGTTATATTAGGTGTGTTCTTTTCTCAGTTGCGTG10.9345794392523363No Hit
TACTTATAGAATAATAAAATGTTGGTTTGATAACAAAATACTCTGGAGTG10.9345794392523363No Hit
GGTGAATGGTTCACAAGTGTCAATAGAATATATGTCATTGACATAGATGC10.9345794392523363No Hit
TCACAACTCAACTCAACCACTCAACCTCTAACCTAAACTTTAACTCCACA10.9345794392523363No Hit
AAATGATAAACCGATTTTATTTTTTAACCAAATCAGGGCTAATGCTATAA10.9345794392523363No Hit

[FAIL]Adapter Content

Adapter graph