FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATGTAGAAAATAGAAAGTAAAGATTATCTTTTGATTATTTATTT15.263157894736842No Hit
TAGTAAATGATATCAAGATATTGTTATAATGCATATCGATTATGCGGCGT15.263157894736842No Hit
GAGATGTATTTGCTGGTATTGATCCAGATTTGGAGCCTCAAGTGGGCATC15.263157894736842No Hit
ATCAACATTTGAGTGATGACTGAGACTCCTTCGATACTTCAAAAAGTTTC15.263157894736842No Hit
CTGCGGAACGTCCGGAGCTTCTTGTGGCTTGGCGTGGGGGGTCACAGTGT15.263157894736842No Hit
CCCAAGTACTCTCTTTCGGATCCCGGAAACAGCTCTCAGAGATCTTTTTT15.263157894736842No Hit
CAAAAAAACTATTAAAAACAAGAAACAATACCAATAATTATCAAAAATAA15.263157894736842No Hit
GAGTAGTTAACCTGAGGGTGATTCGGGATGGCTTAGATTCGGGTGGACCT15.263157894736842No Hit
AATGAAATGTTTATTAAGTAAAAATAAGAACAGTGTAAGAAAATAGATTT15.263157894736842No Hit
GTACTCAACCACCCGCATTATATCCAAATAACTCGAAAGTTTTTAAAAAC15.263157894736842No Hit
GAACATATTGATTGCGGCTTTGCTTTTATTGTGACTTTTAGTTATACCTA15.263157894736842No Hit
GGTTCAAGTATGTCACATATCACAAAGGTAAGACCATCTCCACTACAATT15.263157894736842No Hit
ATACTAATCTCCGGAACATTAAAACAAAAACGAGCTTTTATGATCATTTC15.263157894736842No Hit
CACGTGAACTTGTTACTGATGGTAAAGTAACATACAGATCAGGTAAGAAA15.263157894736842No Hit
AATAGAAGAAAAGGGCCAATCAACAAAAACCGAGTCCACAGCTGGCGACC15.263157894736842No Hit
CATATGTACAAAGTACGAGTTCCAAATATAGACGAATCTAATCATTAGAC15.263157894736842No Hit
ATGCATAGGAAGAAACATGCATTGATTTTCAACGTTGAAATGGTTATAAA15.263157894736842No Hit
CATTTAATTCTTCTTTGAATTATAAAACCAAATCTTGTCGAGTGTTTATT15.263157894736842No Hit
CATCAACTTAATAAAAGCAGTCAACGCAAAGCAACCTCTGATTCTAGATA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)