FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGAAGATGGCATGTGACCGGCTTTTGATATCACACATAACAAAACA24.878048780487805No Hit
CTATTGGCATTTGACGTTTTTTTTATTCAAAAATCATTCACCTTTTTTTT24.878048780487805No Hit
TCTTTATCTCTTGTGTAAGAGCTTCGAGATAAGCAGACCTTCGTGAAGAA24.878048780487805No Hit
GATTGGAGACTTGGACACTTTCATTTGTTTTAACCGTCACAAATCCTAGC12.4390243902439024No Hit
AAGATGGTATATTGGTACCCAATAGAAGTTGCTAACTTGCTTAACCCTAT12.4390243902439024No Hit
TATAAGTACTGCATGAGTAAACCAGAGAAAATCTTTTACTGTAATGTCTA12.4390243902439024No Hit
CCTTATGGACACATGCTTAACCACCGGAGAGAAAATCATTGTAATCTACT12.4390243902439024No Hit
CATATAAACTAATTTGCAACCATCTTTTCTTTATTTCTTTGCATCTTCCA12.4390243902439024No Hit
GGGTGGTAGCGTATTTATGCTATAATAATATTCTCATTCCAGTGGTTGTT12.4390243902439024No Hit
GTATTGTTTTGGTTGGTTTTAGTGTCTTTTCAGGTTTTCACTTCCTTGGA12.4390243902439024No Hit
CCTCTTTTTACCAAGACAGTAGAGCTAATTAAGAGCCTAAGACAGTAGAG12.4390243902439024No Hit
CCATAAATAGTTCGAACTGTATAGGAACAATAATCAATTTTAGCTTCAAT12.4390243902439024No Hit
AAATTATTGACTCTAGAGATATAGTAATATGGAGAAGACAAAATTGTTTC12.4390243902439024No Hit
GTGCATAGCATTTTAGGATCAAATCCACAAGGATTAAGGATTATTCACTA12.4390243902439024No Hit
GTGTATGATTGTGAGCCGTGAATCTTGATGAAGATAGAGAATTCCTCTAG12.4390243902439024No Hit
GGTGATCAGTATGCGGTTATGGTGTCATGTCGGTGCAACTACACAGGTAG12.4390243902439024No Hit
GATTAGTATTAGTTAGTGATCTTGGCGCAGTGAGTCCTTTCTTCCGTGAT12.4390243902439024No Hit
TTATATGCTCATATATTGATGGATCAACTGGAAACACCAAATAAAGATGG12.4390243902439024No Hit
CCATACAACTGATTATATACTAAACACAACTCAACCAATCCAACCATTTA12.4390243902439024No Hit
AGAATTATTGGTAGAAAGCGGTGTCGGAGCTAGTGATACAGAGTATACGA12.4390243902439024No Hit
CATTTAATGCGGGTCGAAGCATATGGTTGCCCGGCTGGTTAAATGCTATT12.4390243902439024No Hit
GAATTTGAGGATACCTGTCTGAGCTAACCCCTTAGGTGAGGTGTAATAGA12.4390243902439024No Hit
GAATAGAGTGCACTATAGATCCGTATATCTTAACAGAGCTAGACTGAAGA12.4390243902439024No Hit
GTCGAAGACACCATCGTGCTTAGACAAGGCAATAAGAACAATCTAATGTT12.4390243902439024No Hit
TAGTAGGATTGTTGAAGCACGAGATAGGAGAAGACCCCTAAGAAGCTAAG12.4390243902439024No Hit
GATTTTCTTAGAAAACGATTATTTTACTCAACTATGAACTGCTATGGATG12.4390243902439024No Hit
ATGTTCCCACAATGGAGAAGAATTTGATATCTGTGAGTCTACTTAATAGT12.4390243902439024No Hit
TTTAATACTCTAGTGCTTGTATCAACGTTACAGGAAATTCTGGTTACCGT12.4390243902439024No Hit
GAGTCAAATTCNTGAAAAAAAGCCGATTGTCTGGGAAATTCTTCAAGAAA12.4390243902439024No Hit
ATATTTAGTTGACCAATAACACATGTGACAACAAATTTGTAAAAAACAAA12.4390243902439024No Hit
ATATAATAATGTTGAAATGTTGATGACAATTTCATACTTCTGAAATCGGA12.4390243902439024No Hit
TACTAATGTGACTTGCAAAGTAAACTTATTTCAGCACATTGAGCATCCTC12.4390243902439024No Hit
GAGCCCAGCTTGAAAATCGAATGTCTTTGGCATTCGAATTGTAGTCTGGA12.4390243902439024No Hit
ATGCCAAAGGAGAATCTAAATTAAACTAGTACTACACTGAGGCGTAACTA12.4390243902439024No Hit
CCGCCTCAGTCATATCTCATTAATTGGAAACCGCAATCAATATTTTGCAG12.4390243902439024No Hit
GTACTACCTTACAAATGCTTCCATCCTTCCTTGGTGGAGTTGCAAGGACA12.4390243902439024No Hit
ACTTTCTTCTATGGATAAAAAAAGATTAAATTGATAGAGGAAGCACCGTA12.4390243902439024No Hit
TAGTATTTGTTACTAGTTTACTTGTAGGAGATAACTCATTCTCTACATGT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)