FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATCAGACATGTCAACAGTCTGAGTAGTTGAAAAGGAAGAAATAACAG30.2437043054427295No Hit
ATAAATATTGTTTATAAAATACAACTGACTGTTTGACCTAAAAAAAAAAA30.2437043054427295No Hit
CATATAAGTTCCCTTTTTCTAGTGTCAGATTAAACACACTGTCGAGATTC30.2437043054427295No Hit
ATAATAGAATAGGAAGTTTGAACTAGGGGTTAGTTGATGTTTTGAGTGAT30.2437043054427295No Hit
GTTTTATACCTTTTTCATATCTCTTACACTCCTTATTCGTATAGGCTCTT30.2437043054427295No Hit
GATTGATGGTTTTGACATAGATTATGGGGTAAAGATAAACTCTTTTATGC20.16246953696181965No Hit
TAGTAATGTCCNCCTCTTACCAAAAACAAGTCATTTATGGATATTATCAG20.16246953696181965No Hit
ATTTATATCTCTAACAGCTTTGCTACTATTCCCAACGGATAGACAAGAGT20.16246953696181965No Hit
GAAGAAGAAGCTACTGAAGAGGATGTGAACTATGTGAATGGTTCCGGTTT20.16246953696181965No Hit
GTTATACGAGGACCCATTTTTATCATACCAATCAAATCATTTTGAGTTTT20.16246953696181965No Hit
TTACAATGGTTGGTATGGAGGAAATATTTCGATTGGCGTATATGGGCGTC20.16246953696181965No Hit
ATAAAGACAAGACAGTTTATTTTCTTTATTATTCTGCTGTAAATTAAAAA20.16246953696181965No Hit
CTCTCAGCTTCTCCGCCACTTCCTCCCTTGGCACCACCCTATCCTCCGCC20.16246953696181965No Hit
ATATATATGTATTTATAACTTATACTATCATATGTATTTATAATTTAAAA20.16246953696181965No Hit
GTTCCTTTTCCCGGTATAGTTTTCGGTTGCGGCTACGACAATGGTGGTTA20.16246953696181965No Hit
GTATAGTGGTAAATGGATATAATTGAAGTAACAATGTCGAACAAATCAAG20.16246953696181965No Hit
TAATTAATTAGAATTCTTGTCTTCGACTTTTGTGATAGTCTAGCCCACTA20.16246953696181965No Hit
GTGCACTGGCACGAAGTGGTGTTAACGAAAATTGTCTGAGAGTTCTTAGT20.16246953696181965No Hit
CAACAAAGGTGAGTGAATGAGATGAATACTGATATATCATGGTTTTTATG20.16246953696181965No Hit
CATTCTTGAGATATTCTCGAGATTGCCAGCTAAGCCAATCGGGAGGTTTT20.16246953696181965No Hit
GTGTATGGTTGTTGAAAACAGAGCTGGTTCTTGCTGGACTAACGCTATCT20.16246953696181965No Hit
ATAGAGTACTGCACCCGAACTTGTGCAAAGGCGCAAGTACTGGCGGATTT20.16246953696181965No Hit
ACCCANTTGATTTCCATGGGATTGTAGTTCAATTGGTCAGAGCACCGCCC20.16246953696181965No Hit
TGGTATCAGAGCATTAAGCTCCTACAGAACTTGTTTCTCTCAAGCGATTG20.16246953696181965No Hit
AAAACTGATGGCTTAATCGATAAATACAAGGTTTCCGACAAAAAAATGGT20.16246953696181965No Hit
CTTTCTCCTCCTGTTTGTCTAATCTTAGCAGGGTATTGACAAGGTGTGAG20.16246953696181965No Hit
GTATTATAGGAATGTTGGTAGGCGCGAAAGGGCTATTGATATCCTGACCC20.16246953696181965No Hit
TTTCTATTGATCGGTCATGTCATATAGGCCCGAGTCGGACATCTAATTGC20.16246953696181965No Hit
GGAAGCAAAGATGTTACGATGAATGTCGATCGTACTGGAAATGGAGGAAC20.16246953696181965No Hit
ACTGAGAGGTCCACTAGGGATCAGAAATTGTTGAAGAAAGAACAAGATGT20.16246953696181965No Hit
GTTCCTATGTCGATATGGTGGTTGATCCCTCAGTATTTGTTGCTTGGAAT20.16246953696181965No Hit
GCTTTATAATCTGTGTCATAACGGTGGAAGTGACGTCGTTGGGGCACTCG20.16246953696181965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)