FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAGCTCTCTCTAACCTCCCAATCTCCNCAGTAGCAACTAGTTTTTTA14.3478260869565215No Hit
TGTCTACCATTTGACCAAAAACTCATATTGAAAGAGAATCGACTTCCATA14.3478260869565215No Hit
GATCTTTGGTATCCTTTTCTATACTGAGATATATCATAAGACTAATAAGT14.3478260869565215No Hit
CTTGATAAGCTTGTCTTATTTCTTGTATGATAGTTTATGGCTGTCACCAA14.3478260869565215No Hit
GGATAATGTCTTGTGGGATCTACAAGCTGATGATCTCATTAATCATATGG14.3478260869565215No Hit
GTTTTACCCAACATCTAAACTTTGCAAGTTCAAGGTTGTGCGTGGCAGTA14.3478260869565215No Hit
TAATTGGGTTCTGAGACCCCATAAATTAAATCGAAATTTCTCATAATTTA14.3478260869565215No Hit
GGGTGAAGGGCGGTAAGACAAAGAGAAGGCGCAGGAAAGAACAAGGGATT14.3478260869565215No Hit
GCTCTGACTTCTGAGGCGAGTCTCAGTTCGGCAATTAGAGCATTATATTC14.3478260869565215No Hit
GAATAGTTCCGTTATTTTGTCTAAGTGATTGTTGAAGAATGCCAGCCTTG14.3478260869565215No Hit
CAATAGTACAGTATGATGTGGATACCTAGGGATAGGCATGGATTTTGGCC14.3478260869565215No Hit
CCTAAATATAAAAGAAATTTTCCTAAAGATTCATAAACATTTAGGAAAGT14.3478260869565215No Hit
CGTTTGAGTGAGGTTTTGGGGTGTGTAGGGGGCGCGGGGGGCCTGGGGGG14.3478260869565215No Hit
ATTCTAATTCGTTGTCACGTAATGAATAATAAATATTCATACTTTTTAAT14.3478260869565215No Hit
ATACATACGTAATCTAGATATACAAAATCTAAAAAAAAAAGAAGATTAAA14.3478260869565215No Hit
GCTAACTCATACCGACTTTGAACTCAAATTGGATTTCATCAGATTGTTGG14.3478260869565215No Hit
ACTCTTAACGGATCAAAATTCCANATTCATGTTCAACAACTCAAAATCGA14.3478260869565215No Hit
GCCTTCTAGATGCGGTATTTCTTTAGTGATCCGTTCAGGACCTTGGGTTG14.3478260869565215No Hit
CAAAATAGGTATGCAGAGGTGTTTGAACAGTAGGAACGTAAGTATAGCCC14.3478260869565215No Hit
GTTTTGGGGTTGGGGTGTTGTGGGGTGTGGGAGTGAGGGGGTGGTTGGCT14.3478260869565215No Hit
GGGGGGTGCGGGGGGGGGGGGGGGCGGGGGGGGGGGGGTGGGGGGGGGGG14.3478260869565215No Hit
TCTGGGGGCTTTACCGGGACTAACTAGTAAAAGGCCTAAGGCAGGAAGCG14.3478260869565215No Hit
GCTGGAGCCCTTTCGCAGCCGCTTACCCTCGGGAGGGTGAGGTGGTGGGC14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)