FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTAGGGATAGCTTTGAAGTCATACATAATATGATTTTCTTCGTCAAT43.9215686274509802No Hit
ACTATATTTTTTGTCATAATCCGCATTTTCAAAGAAAATAGAACGATTTC21.9607843137254901No Hit
TTAATATCTGAATCTATTCATGTCAGGAGAGAAATAGTGAATATGGTTCA21.9607843137254901No Hit
ACTGATTCCAGGGCGTTTTGTTGTGTTACCGATGTTTCCCTGAGTTCTCC21.9607843137254901No Hit
ACACAAACGGATCTTAAACTTCTAAACCCGAACATCCAAACCCAAACTCG21.9607843137254901No Hit
GTTAACAATAGNAGAACTTTCATTAATATACGTGACTACTACGTATATCA21.9607843137254901No Hit
GTATGATACTTATGATATCGATGAAATTTATTTTCAAATCTTTCTTCTTA21.9607843137254901No Hit
ATACCACTGATTAAGATTCGTGGTTACTCTCATCTCACTGATAACTTTGA21.9607843137254901No Hit
GAATATAGCAGAGTATTAAGAGCTATTGTAAAGTTCTGAAAAATGCTATG10.9803921568627451No Hit
CTTGAGAGTATAGCATCTCCTGTCACTGCCTGAAGATTAAAGCATAGTAG10.9803921568627451No Hit
GAATCGAATGCCCCTTGGCGTTCCGACCCAGTTCCAGCGAGGCACTTTTC10.9803921568627451No Hit
GTTCAACACCCATAAGTAAGCCACCCTTTTTGGGNTTTCTTGCNTCCCAA10.9803921568627451No Hit
CAAAGCAGCAAAGACCTGTTTTGAGACAGTTTCAAAGGATCAGAAGTTTG10.9803921568627451No Hit
GCTTATGAAACCTTACCGTGTTTGATGTAATTTGCCGAGAATGACAGGTG10.9803921568627451No Hit
GTTTGCCTCGATTTGGTACCGCTCTCGCGGCCCGCACCGAAACAGTGCTT10.9803921568627451No Hit
TAAAAATGGTATGACCTGAAAGTCCAACAAATAAGGAGAAAGTTAATATA10.9803921568627451No Hit
TGAATGATGTGGTTTGGGTCATGTGAGAGCTTCTTGTCCTTTTCAATTAG10.9803921568627451No Hit
ATCCAGTCCAAGTCATCTCCTCCGTACAAGAAGATGTAATTATCCGGGTT10.9803921568627451No Hit
GCTTTTAAGTTTTGAATAAAAAAATTTGGGTTTACAAACTCCAACTGCAT10.9803921568627451No Hit
ATATTTTCCAAAGAGCTTCATGGGATATAAGTATATAGCAAGAACAGTGT10.9803921568627451No Hit
GATTGAAGTAGTGTTCACGATATATTTTGTTAATCTTAATAATCTTGTGA10.9803921568627451No Hit
CTCCTATGTTCCTTTCATTACAGTATTTCTGAACAAGTTCCTGGATAACA10.9803921568627451No Hit
TATATACATACTCTTACAAGTCTAGCTAGAAATTCTCCGTTTAAAATTAT10.9803921568627451No Hit
GGCATTANGATATTCACCGAATATGGATTCGGATCAAACAGAACNACAAA10.9803921568627451No Hit
CTATTTTCCAGCCCATAATCCTCATGTATCATTCGAATGCAATCATCAGA10.9803921568627451No Hit
AACTACAGTGGCATCAGAGTCAGCGTTTAGCGTTGGAGGACGTGTTTTAA10.9803921568627451No Hit
ATATAGTTCCGCTTAAAAACTATTTGGATGCTCAATACTATGGTGAAATT10.9803921568627451No Hit
ATCCCAGCTAAGGAACCGGCCCGCCGTGAGTGATGATCTTNGGTTCGCGG10.9803921568627451No Hit
GGCTGTTGCGTTGGGCCGAAGGCGTTAAACTATTAATTTGAGTTCTTTCC10.9803921568627451No Hit
CATTGTCCACGAAGTGTCTGGAGATAAGTAGTGTTGAGAGTAGGATCTGG10.9803921568627451No Hit
ATCCCAGCTAAGGAACCGGCCCGCCGTGAGTGATGATCTTTGGTTCGCGG10.9803921568627451No Hit
TTTTAAATCCATTCGGTTCGGTTTCCATCCGGGTTTTTCAGATCGGTTTC10.9803921568627451No Hit
TTACAAGACTCTCAAAGCAAAAAAGCAAGTAAGGAACAGCTTCTGAATCA10.9803921568627451No Hit
TCTAATAACAGTGGACATGACTTTTTTTTCTGAAGGTAGGAGAAAAGATA10.9803921568627451No Hit
GAGCGAAGAAGACTAGAACCCGAGGTGAGACTTCTAAAAGCCATCTCAGA10.9803921568627451No Hit
GCACTCTCGACCAGGCACTCGAGTGAAGCACTCTAGACAGGCACTCTCGA10.9803921568627451No Hit
CTTTTACTCAAATTAGTGATAATTTAAAAATTGAATTTACCTATTAATTT10.9803921568627451No Hit
AAGGGGGGCTGCTTACGGGCCAGGCCACTTCTCTGAAAAGACCTGTTCTC10.9803921568627451No Hit
GTAGTTGCACTATCATCTTAGGATTGAGTTTTAGAAATTTTGCTTAGGAT10.9803921568627451No Hit
CGGTAGGAAGAAACTTCCGATGACCATTTGTTTATAATCTCTGAAGTAAT10.9803921568627451No Hit
GTCGTGGATGAGTGGGCTCCGTAAAATCCAGTAGATTAAGGGATGTAACA10.9803921568627451No Hit
GTATTGAATCCGGCGTCCTATTTATAAGGAACCAAAGAAACTGAGTTTTC10.9803921568627451No Hit
GAGAAGGATTGNTCTGAGCGAAGGAGAAGATTAGCGACTGGAGATGGATA10.9803921568627451No Hit
CATCATCCATCGCTAAACGATGATTAAGCTATATTAATTAATTAACGTTT10.9803921568627451No Hit
TCTATCCTCTAAACAAAAGAAAACCAAATCTCTTTGCGAATCTAAATAAA10.9803921568627451No Hit
GTTTACAAACCAATCATTCACTTTGCTTTTCATTCATTCATTTGCATTCA10.9803921568627451No Hit
GAATTCGATTATGTCGTTGAGAGATTGGAGCCTGTGGCTAAGTTCATCAA10.9803921568627451No Hit
CGTTATATCAATAAGTATGAGTTGGCTCAAGATTGGAGTTATCGTTGCAA10.9803921568627451No Hit
ATGTCGGATACAAAAAATATTTTGGAATTTTTGCCGGCTCGACCACTCAC10.9803921568627451No Hit
CTTCTTTTCCAATCCAGATTCTGTTAGCATACATCATATCTTATCAGTTA10.9803921568627451No Hit
ATTTCCATAGCTCACTCATATATGATCAATCAATACATTTCGAAGTGATA10.9803921568627451No Hit
AGTGATGAGTTAGTTCAATCAGAATTTCAAGTTTCTATTGCTAGCATATC10.9803921568627451No Hit
ATGATTGATATGGTTCGTTATTCTGTTCTTGAATTATGACAAGTTCATGA10.9803921568627451No Hit
GAATCACAGTGTAGATGATGCTTGGGACGTGTTTAAAGATTTCAAACGTC10.9803921568627451No Hit
ATCCTTCTTGGTTTTAGAGTATACGTTTCGTATATTCTCTTTTTTTTTTC10.9803921568627451No Hit
CTAAAAGTGTGTTCAAATCTTTGATTAATAAAATGTGTCTTCAAATCTTT10.9803921568627451No Hit
ATCTCATCTAGAGACTGTGGCACTGATGAAGTCAGTACTCTTGTGCTTGT10.9803921568627451No Hit
TTTTAAGATGCTGCAACATACCAGTCTCTAGATTTCTTCAGGTTTGAATG10.9803921568627451No Hit
GATTTGAGCAATTTTCAAAAAGTCTAATTATATGCTTTCATTTTAAAAAA10.9803921568627451No Hit
GGGTGCCAACTCGCAGCCACAGAACCATAAAAGTAAACAAACACAGACTT10.9803921568627451No Hit
AGAAATAGGTGTACGAGACCATCCTGGGAACCGTGTTTTTGGGGGGTAAC10.9803921568627451No Hit
CGTGAGTTGCAAGTAAGCGATGGAGATCAATTTTGAAACGTGACCTGTTG10.9803921568627451No Hit
GTCTCCACCTAGGGTTTATGCTTTAGAATTAGAACCACCCAACCTTCCAA10.9803921568627451No Hit
ATATAGACGTGTGAAAGAAGGTCTTATACGACGTCACTCCAATGCTTTGA10.9803921568627451No Hit
AGACCAACGTTTGGTCAACAAGATGTTTGCCAATCAGCTCGACAAAACTA10.9803921568627451No Hit
ATTCAAATTCAGACCATCCATCGGATCAACCAAATTCATAGGAACCTGAT10.9803921568627451No Hit
TTGTATACTTTTCTCCGTGTAAAGATGAAATTACTGTCTTCTTGTCATGT10.9803921568627451No Hit
CATTAACATTTCCCTTTTTTTCATTCTAGTTATTAACATTAGAAAGGGGT10.9803921568627451No Hit
GCTATCAACAAGCAATCTCATCGCAGGAGTACCCTAGACAACAGACTTCA10.9803921568627451No Hit
AATATATTTTTTCCCAAAGCAAGTTCCCCACCAACTGTAGCAGCACCATC10.9803921568627451No Hit
ACACACACGTGGTGATACTGGACCAATGCTAATGATCAATAGCGTGTGCC10.9803921568627451No Hit
TATATATAGTTTGCATAAGATAATTCATTAGTAGATTTTGTTATTTATCT10.9803921568627451No Hit
CTTGTGTATCTGTCAAAAGAAACAAAGCCAAAGAGTGATTTTAACTCATG10.9803921568627451No Hit
AGATTCCACTGACCCGTAAAAAGTGGCCCTAACCCTTGGGGATGTGGTAA10.9803921568627451No Hit
ACTTTTCTTTAAAAGAATTTTTGTTTTTTGGTAAGGGCCCATGGTAAATG10.9803921568627451No Hit
GCGGTAGGCGTTATCATTGATCTTTTCGGGGNTTAGGAATGGCCCGTCTC10.9803921568627451No Hit
CTTATGAATCTCAAAATCTATTTGAATCTTCTTGCACTGAATTCACTCAA10.9803921568627451No Hit
AGTGCATACCATGATTCTTCTGTCTATCAATAACAGAATGCATTGCACGG10.9803921568627451No Hit
GTCAAAGGAGAAGTCCTTACATCTTGTTATTTTTGGATCTAAAGAAGTCC10.9803921568627451No Hit
GGTCGAGTGAGCCGGCTGTCTTCTCTGCAATCTTCACTCGAGTCCATAGG10.9803921568627451No Hit
GGTATAAACTTTATGATTTGTTTCCTTTTTATTTTATTTTTTTTACCCTC10.9803921568627451No Hit
GATTATGCNCTTTTCCTTTCATCTTAGACTTGGGGCTTGGGGGAGAGTTA10.9803921568627451No Hit
GCCCCACCCTTATCCGACGGTCAGTCCGTTATTTTTAGAGGTTCTAAAGA10.9803921568627451No Hit
ACCCTAAATTTTATAAGAATTCTGTGATTGCCACGTGTCATTCTAAACGT10.9803921568627451No Hit
TGGATTTACTATGAATATTCGCATTCAAGCCTTTCTCCANTTGTTACTTG10.9803921568627451No Hit
AGGTCCTCCTATCCTCATTCCTCTATGAAACCTTGTGAGCCATGACCATC10.9803921568627451No Hit
TATTTAATACAGTTGTTTTCATATACCGTAACTTAATATATTAAATATAA10.9803921568627451No Hit
CCCCGGTTCGCACCGGAGGAGTACGCCCTGCTAATGTGCCTTGGATGATC10.9803921568627451No Hit
CAAATAATGAGCCCAACATCAGTACATCACTGCTATTAGTGATATAGTGA10.9803921568627451No Hit
GGTTTGAAGATAAGTCGAGAGATCGTGAATCTCCTCTCACCGTCGGTGCA10.9803921568627451No Hit
AGACAATATCGATCTCATGAAATGTTCGTTCAATAGCCTCCAGAAGAAAG10.9803921568627451No Hit
GTCATACACAACCAACAACCAAAAAAGCCCATGTTATCACCTTAAGGAGT10.9803921568627451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)