FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGATTAATCCGAATTTGGAGTTTTTTTTTTTTCATTCCACATAATT25.88235294117647No Hit
TTTCAACGTCAAACATTTGTCGTCCGATCGATCTTTTTGACAACCTTTTG25.88235294117647No Hit
GTTAATTATGTCTAACTTTTTTTTAAGATGGTATTTGTTATCATCTTCTT12.941176470588235No Hit
CCGTCGTTAATCTTGGCCATCCAACAACGTTGACACATTAATGAAAGGCT12.941176470588235No Hit
ATAAAGAGGTGTAAATTGATGCAGAGCTCCTTGAGAAGATTCCACGAGAA12.941176470588235No Hit
TTCTTAGGAGATTCTTCTTTAACTTTCTCTTGAGTGCTCTCTTTATCCAC12.941176470588235No Hit
CGTGCAATCGCCTGTTGATAATTTTGGAGCCAAACTAGGGTTTGGTGTCG12.941176470588235No Hit
GGAGTATGAGAGTTCAAAGCGTGAAGCCTATCTTGGGATGGTTATTAGTT12.941176470588235No Hit
GCTAAATAAAGTTAGACAATACTTATAAGTACAAAAACAAAAATAAAAGA12.941176470588235No Hit
CCTCTGGAGATCTCGTCCTTGTCACCCTTGTTGTTGATGCATTAGTGTCC12.941176470588235No Hit
ACTAATAATTAATCACATTTATATTTTTTCTGGCTGATATACAAAACAAC12.941176470588235No Hit
ACAATGTTGGATCGCTAACGTTGTTGACGTTGTACAAATAAAACTTGTTC12.941176470588235No Hit
TTCATCAACCTAATAAAAGCATTTGATTCATAGAACTAGAGATCTATAAA12.941176470588235No Hit
CTTTTAATCCTTCTGGTAATGCTCCTAGCTTTGTGTAACAAGCAGCTCTG12.941176470588235No Hit
CCTTTCCTGTGCATCAGGCGGAGTATACTTTCTGATGCAATCTTCATATG12.941176470588235No Hit
ATAATGATGAATAATCAAAGAGGAGTATTATTGATAATTATATGATTATC12.941176470588235No Hit
GGCCAAGACACTGTATGTCTGAATTTAGTGGAACATAGACTCAAATTAGA12.941176470588235No Hit
GGTATAAAAACACCATTCACAATTTTTAGCAAAATTTTTTTATGGTTTTG12.941176470588235No Hit
GGCTCCAGCTAAAGTTGCAAAAGTTGAAATCCCTTCTGCCCCTCTTGGTG12.941176470588235No Hit
TCCTTGATCTTCATGCGTTGCATGGAAATGTTGTCCATAAGCTTTGTGGG12.941176470588235No Hit
TATATGTAGTTATTATTTTTACGACTAAGACTTGAAATTTATTATTGCAT12.941176470588235No Hit
ACAACTTCTTCGGCGGAGACTTCCAAGAGCTGTTGGTGCTGATAGCTTAC12.941176470588235No Hit
GCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTTCAC12.941176470588235No Hit
CCCACAACTCGATCAAGAAAAGATTCAATTTTTGTAGTTGTTGATAGGTT12.941176470588235No Hit
CTCTTGGAGAGCAATATGCTCAGTAAAATTTCTTAAGAGGGGTCATGAGG12.941176470588235No Hit
TGTATGCACCAGTTGGCTTTACCTTTGGCATTCTCTTATAATTTTATCTA12.941176470588235No Hit
GAATGATTACTTTGAATGTTTGTCTTAGTTATGGCTGAGAGTTTATGCAT12.941176470588235No Hit
CAAATCAACGATGGGTTCTATTCATTCCCTAGTTAATGNGGTACTTTAAT12.941176470588235No Hit
GGTTAATTACGGGAATTAAAAAAAAAACATATTTATTGTATTTTAAATTG12.941176470588235No Hit
GCATTAGTGTCCCTGAAATAATGATTATAGAAGCTTAGCTACATGTTCTT12.941176470588235No Hit
CCAATTATCTGATTGATGTTGTTCTTCTCTCCTTCGTTTTCCTGTTTTCA12.941176470588235No Hit
GCTCTTTCTCAGATGATTCTAGTTGATTGATCGGAGCTATCTCAGCGAAC12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)