FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCTTTCCCTTTCTCTCTTTTGTTTCCTTTCTCTCTCTCTCTCTCT22.5316455696202533No Hit
GTTTAGTGCAATCAATCACAATGTATCAATGCATAATAAGATAANTCAAA22.5316455696202533No Hit
ATTTATTTGATTTTTTCCATGTTGTCTATTGCGTTTCTTTACACCATGAG11.2658227848101267No Hit
GGATATTGACAATTTGACATAGAAATTATGTCTACAACAAGTTGATGAAG11.2658227848101267No Hit
CAAGTATATATTTTATACACATCATTTACATGAAATGACCTAATTAATAT11.2658227848101267No Hit
ATTCTACGAGGAGGCTTTCGGGTTTTGATGCGCCAGGAGCGTAGGGCGTC11.2658227848101267No Hit
AATGAGCATTGTTCAGATTTACAGTGATCCTGGAGTTGAGTTTCAAAATG11.2658227848101267No Hit
GCACCTATTTTAGGCCCTGGCGGGATACTCCGCGTGCATCGGCGCCCCGC11.2658227848101267No Hit
GGCGTGGCGTGGGCGGGTGGTGGGGGGGGGGGGGGGGGGGCTGCGGGGGG11.2658227848101267No Hit
GGTCAGTCGGGTGGGGTTTTGGGGGGTGGGGGTGGGGGGGGCTTTGGGGG11.2658227848101267No Hit
GGGCTGAACATGCTGAATCTTTCCTTTATTGCCGGCGAGGCTGCTAGACT11.2658227848101267No Hit
GGACAGTGTCTTCACTCTGCATTACTGTTTACAACCCAACATGCTGGCTA11.2658227848101267No Hit
GGATACTTTCCTTGTAATAGTCAATAACAAGCTGTACCCTGTTGTACTTG11.2658227848101267No Hit
GAATCTTGACTCTTCTAGCTTTACATGGATAGAAAGAACTCCAAAAGTTT11.2658227848101267No Hit
GGATAAGCTGTGATGTTCGAGATATTCTTTTTCGTATTTAGTGGGCATTA11.2658227848101267No Hit
GTATATAGCTATAGGGGATCATATAAAGGAAAGAGATGTTATTATTTTCG11.2658227848101267No Hit
TTTGCAAGGTTGTGAATGTAGAACATAGGGGCGTCCAGTTGCTTTTGTGA11.2658227848101267No Hit
GCGGGGGGCCGGGGGGGGTTGGGGCGGGGGGGGGGGCGGGCCGCGGGGCC11.2658227848101267No Hit
AGATCCAGTCAGTGCATCATTGATTCCTTCGGACGTGTCACTGGGCCGAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2658227848101267No Hit
GGGGGTGGGGGTGGTCGGGCGGGGCGCATTGTTTTGGTAGAGCGGTGGGG11.2658227848101267No Hit
GGTCCAGCCTCTTGGTGAGTCCGTTCACGGCCACCTATGACCGAGGCTCA11.2658227848101267No Hit
GTAGTCCCATGGGTCTCATAGTTGATACAACGGCCATACTCAGTCATATC11.2658227848101267No Hit
AGGCTGGGATGGGGGTGGCGTGGGGTAGGAGGTTGTTGTGCGGTCGGGGT11.2658227848101267No Hit
TTAGCTGTCTCGGTTTATAGCAAATTTCTTGCTTTTCTTGTGTGTTGATA11.2658227848101267No Hit
CAAAGACTAATATGAAAAATACAATTCAAAAAAAATAAATGAAGNGACAA11.2658227848101267No Hit
ACATTGGCGTGTCTAGTGAAGTTTGCAGATGGAAGGCACGTGATGGCTNC11.2658227848101267No Hit
GATATATAGGATCTCTGCTNGGGTTCGGGGGGTTGCTGCGTGTGGGGTGG11.2658227848101267No Hit
TCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCA11.2658227848101267No Hit
AAACTATCGATACTTAGGTCTTATAATACCTCAGGAGCTAATGAAACTAT11.2658227848101267No Hit
ATGGTAGGGTTCGCTGGTGTGGGTGTGTTGGGGGGGGTTGTGGGTCTGGT11.2658227848101267No Hit
GGGTTTGTGGGTTGGGTTGGGGGTGTGGGGAGGGTTTGGTGGGGGGCGCT11.2658227848101267No Hit
GATCTGGCCATCCCTTTGCGGCGGTTTGCCGTGGGGGGGTGTTTCGGGGG11.2658227848101267No Hit
ATCTNATAGGAAATGATACTCTGAATCANAGAACTATAATGAAAGATAGG11.2658227848101267No Hit
GTCTCGTTTGAGAGGTAGGGATGGGCTTGTTGGGCGGGGGGCTGGGGAGG11.2658227848101267No Hit
GTGGCTCAGGACGAACGGTAGAAGAAGGTCGAGGAGGCTTCATGTTCCTA11.2658227848101267No Hit
TGTCCCAGTTTAAAGCCAGCGATTGATCCAACCCGGAAGTTAAACCGATA11.2658227848101267No Hit
TTCCATAACAATTAAATGAAAAGTAAAATGGCTCAAGTACTGTGTCCAAA11.2658227848101267No Hit
GTGGTATGCCATATGCCCATGGCGCCTCGCTGTGTCATATTTGTTTTTTT11.2658227848101267No Hit
TCGGGGGGGTGAGGGGGGTGGGGGGGTCGGGGGGGGGGGTGCGGGGGGCG11.2658227848101267No Hit
CTAGAACGTTGGTACCAAATCTTCTTGAGTCTTCGGCCCCTGAGGAATTG11.2658227848101267No Hit
GGCTATAACAGAGTTTCTGTTATTCGTATTAACAGCTACTCTAGGAGGAA11.2658227848101267No Hit
GCGCTCGAGGGGCGGAGGGTGGGGGCTTGTGGCTGGGGGGGGCGGGGGGG11.2658227848101267No Hit
ACCTATTATTATAAAGTACCATATGATCTCCATTTACCGACAACTGATCG11.2658227848101267No Hit
CCTTTTATTTGGGAACAAACAGCAAAGGTTCATCATCATCATAGAACATT11.2658227848101267No Hit
ATAAAAAATAGTAATAAATACAACATAATATCCATTTATAGAGCAATAAA11.2658227848101267No Hit
AGATTGGTCTGGGCGTGGCGTTGGTGGGGGGGGTGGGGGGGGAGGTGGGT11.2658227848101267No Hit
GGGGGCGGGGGGCGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGCG11.2658227848101267No Hit
CAATCCACCTTGACATAGTGTGGCGGCTTTCCTCCACGGATTAGCTTTTC11.2658227848101267No Hit
GGGGGTGGTGGGGTGGTGGGTCGGGGGGTGGGGCGGGGGGCGGCGCCGGG11.2658227848101267No Hit
TGTTAGGACATTGAAAAAGAAGAGCTTCCAGCTCAAATGGTTCAAGTTTC11.2658227848101267No Hit
GGACCAACCTGGGACAAAAGGAGCCGGATGCGGAAAAACTAGGCATTTTC11.2658227848101267No Hit
CTTGCTCATCTTTTCATCTGATATCACATAAGTAAATGAAAAATAAGTCT11.2658227848101267No Hit
TAGTCAACTTTAGTAACATAACTGGTAATGAATAAAACAGATCGGAAAGC11.2658227848101267No Hit
CTTCCCGGTTCGCTCGCCGCTACTAAGGGAATCCTTGTTAGTTTCTTCTC11.2658227848101267No Hit
GTTCGATTCCGATAGCCGGCTTTTCCATATTATTTCGTTTTTTTACATTT11.2658227848101267No Hit
CTATTTCTTAGTTCAAGACCCCTATTACTAATACTAACTGGAATCAAAGA11.2658227848101267No Hit
ATGCAAAACCAATGAAAACTAAATGCAACAATGGTGAGCAATGGGTGTGG11.2658227848101267No Hit
GTCCATGGGAACGGTAGTTGTCTGTTCTGGGCCCGCGGGTAGTGGGTGAG11.2658227848101267No Hit
ACGTACGGGTGTCCGGGGGGGGTGCCTAGTGTGGCGGGATCGGGCGGGTC11.2658227848101267No Hit
ATTAGGGGGTTTACATAAAATTATCAATAATTACTAGTATAAAGATCATA11.2658227848101267No Hit
TGTATACATATCTTTTGGCCATACATTATTTGCGGCGGATTCCACATGCA11.2658227848101267No Hit
ATGTAATGCTTGCAGATGGTCGTTGGCTATGTCGAGAATGTCTAGAATGT11.2658227848101267No Hit
GATATATGATGTATGATGTGTACCTGTTGTGTGCCTGGTTGGCGTGTGGG11.2658227848101267No Hit
GGCCTAGTCTTTCCGGCAATTGCAATGGCTGCTTTATTTCTTCANATTNA11.2658227848101267No Hit
AATTACAGATCTGAAATGAGAAATAAAAATGGCAGAGTAACAGTAAAATT11.2658227848101267No Hit
CCTCCAGGGGCGGGGGGGGGGGGTGGGGGGGGCCGGGGGGGGGGGGCGGG11.2658227848101267No Hit
CCCTTTAGCTTCTTTTGCGCGTCTTCAAGAGCTCTTCTTTCTCTGAAAGG11.2658227848101267No Hit
GAATGAGCAAAAACTATAGAATGTCCGCCACGGAGTTTCAAAGCAGTTTC11.2658227848101267No Hit
GTTGTCTTCGAACTACTTCATCTCTAATGTTGAAGACGTGATGGTAAATC11.2658227848101267No Hit
CATCTATATTAGTTTGAGTTGAGTATTGGTGCAGTTTCTGTCTCTTATAC11.2658227848101267No Hit
AATAAATTGATCTAAGAGAAACATCACAATATTGATAATAAATTCATTAT11.2658227848101267No Hit
ATCGCACTAAGGCGCATAAGACAGTACACTTCACCGATTCACCAAGTAAC11.2658227848101267No Hit
TTACAAGGCTCAGGTTAAGCTTCCTTTCAATGCATTTGGGACTATGGCTA11.2658227848101267No Hit
GATTCTTCCCTATCCATATTCCCAACTGGCGATTGGTGGCTCTGTCCTTC11.2658227848101267No Hit
CACATAAATCGTTTTAAAACCATTGTAACTTCAATTTCTTATTCGTTGTT11.2658227848101267No Hit
GTGTGGGTTGCGTGCCTGGTGGGTCTGGGGGGGGGGGGGTGGGGTGGGGG11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)