FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGTTTTGTGCCTTTGGGTGTTTGGTGTTTGTGTGAGGCGTGGGAG12.2222222222222223No Hit
GAATTAATGGAGGGTAAGGCGGTTTTGTGAGAAGATCACATGTCTTGTTT12.2222222222222223No Hit
CAGTTTTCGAAGTCTGGTTCCTAGAGCATTAAGGAGCCTTTTGCCCAATA12.2222222222222223No Hit
AGAGTATCCCGCTTTGGTTGATGAAGAGAGGTGTAGTTTCAATGATGATG12.2222222222222223No Hit
ATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTT12.2222222222222223No Hit
CCTTTAGAACCTTGGCTGCTCGAAGCTGAGAGTCCTTTGAAACTCCAATC12.2222222222222223No Hit
GTCATAGCACTTGTAGCCCTTTTGGGTAGTAGAGTAGCCTAGGAATATGC12.2222222222222223No Hit
GCTTGGAGCAGTGGATATGAGGTTGGGGCTATGGAAAGGAATCATACATG12.2222222222222223No Hit
TCCACTTAGCTCCATGGAACAATATGCTACTACTGAAACATGGAAGAATT12.2222222222222223No Hit
ATATTAGTCGTCCCAATGGCGTCCAATTATCAAACCAGAATGACGTTGAG12.2222222222222223No Hit
CTCTTATCTAATGGTAGTTCTCTCACAGAAATTGATGATATGCCTCAACC12.2222222222222223No Hit
ATATTATATGGTATGAAGAAAAGAAAAAGAAGAAAATAATTGGATTTAAA12.2222222222222223No Hit
ATATTGCTCGCATCTAATTGCGCCACGTCTGTGTTAGGAGGAAGATCAGC12.2222222222222223No Hit
ATCTTAATGCGGCGATTATACGACGGTCTCGGTTCCTGGTGCGGAGAGGA12.2222222222222223No Hit
GACGTGGAGAGTGGAAAGGAGCTCATAGAGAACTTAGCTCTAGCTGATGG12.2222222222222223No Hit
GTAGAATATTGTATAACACATAGATTCCTACAAACATAAATAAAAAAAGC12.2222222222222223No Hit
TGGGAGACCAGAGTCTGGAGGGACAGCAGTCTCTGAGCGTGGGGTTGGGG12.2222222222222223No Hit
GTATCGGGTGTTGGCACTTCGGTTGTTGCCAGTCCTGTCATTTCCTAGAA12.2222222222222223No Hit
AAACTATTGGGTATGGACAGACAAACAGACAAGTATATATTCAAATAGTA12.2222222222222223No Hit
ATGTTATACTATTAAATTCTTGACGATGAATCGATTTTATAGCTCCGATA12.2222222222222223No Hit
CGTACGAAAATAACTAGACTTAAGTCAAAAGTAATTCAGTATAAGAATAT12.2222222222222223No Hit
CAACTTCATTGATTGAGCATTATCTACTTAATTCAGATAGCCAGCCTACT12.2222222222222223No Hit
TTAGAAGGGATTGCATCTGTAATTATCCGCTTTACTGTAGAGTTACCAAT12.2222222222222223No Hit
GTATTACTACATCTAACACATAATTAAAATGCGATAATTTGTCTTCTAAA12.2222222222222223No Hit
TCTCTGAAAATCTCTAAATTTTAGGTCTTGGAAAACGAGGTAAATCCTGG12.2222222222222223No Hit
GGACTCCTTCTCCTTCCCCTTCTTCAAACTCCGATTCGTATTTTTCATAG12.2222222222222223No Hit
ACTCCGCGCCGCGTCGCAGGGCGGGCTCGCCCCGCACGCCCCTGGGGGCC12.2222222222222223No Hit
CCTCATGAAGATGGTTGAGGAAGGTAATAAAGCTATGAAGAGAGTTGTGA12.2222222222222223No Hit
ATATATGGAGGTTCTTGGAGAGAGACACAATAAAATATCTTAGCTTTCAT12.2222222222222223No Hit
GACCAATCAAAAATAAATAGGCACTCAAAACAAGTACATGTTCGAGTATC12.2222222222222223No Hit
TTTCTTGAGTGACTCCACATAGTGCATAGTCGGTAAATTATGAATGTATT12.2222222222222223No Hit
ATTTATAGTCTTCATGATTTTTTTCATAAATGTATGGGTGTGTGTGCGTG12.2222222222222223No Hit
GGTAGGGATCATCAAAACACCAAACCATCCAATGTAAAGACGATTTTCAG12.2222222222222223No Hit
TTGAAAAAGTGAGCCCAGTAAAATAAGAAAATTTGTGGGTTGATGACCAA12.2222222222222223No Hit
CATAATAACCATCACTCTCACTTATCTATCTTCATATAAATTAAATTGTA12.2222222222222223No Hit
CAATAAAACTAAATCAAAGATAAAATAAACATATTAGTAATACAAAAAAT12.2222222222222223No Hit
GTATTGAAGCCCAAGGTCATAGTGGTATTGGGAGCTGGGATAGGTGGGTT12.2222222222222223No Hit
TCATACCACTTTCTATAAATACCACTCATTCTTTGTTATTTATCATATGG12.2222222222222223No Hit
GTATTTGGGCTTAAGTGACGACCATCGGGTCCATATCTTCCTTCATTTTA12.2222222222222223No Hit
CTGTATTCCTCTATCCGTAGAGTACATTGAATGTACAGAAATGGCTATAA12.2222222222222223No Hit
GTATTAAATGTAACATAAACTGTAGTTCAACATAAATATACCAAAGAAAA12.2222222222222223No Hit
AGACGCCGGTTCGTATCGTAAGGACCGTTTGGTCTGAAAAACATTGACTC12.2222222222222223No Hit
AAAGTCCACAGAGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAA12.2222222222222223No Hit
GGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAATTCCGAACC12.2222222222222223No Hit
CCCGTAGGTTACCTATCCAATTGACAACTCTACCAATGTGAGATATTGAA12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)