FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGATCTGGGATCTGTTATGGTTACGCATCATGAAAACAATGTCTAAAG17.6923076923076925No Hit
GAANTTGAATACACANATTTCTAAGATTACAGACAATTCAGAAAATATTT17.6923076923076925No Hit
TGGTTAACTTTATTATGCCTATTGCAATATCATATGAAAAAAAGAAATAT17.6923076923076925No Hit
CCGTTTATATCGCTACAACAAAATTATTTTTGATATGTCCAAACTTACAA17.6923076923076925No Hit
AGCAATCAAAAACTGTAACCCAAGCACCCAGACCCCGCCAATCTTACACA17.6923076923076925No Hit
ATTCTATAATAAAACAAAAAAACTTAAAAACAAAATCAAACAACACCAGA17.6923076923076925No Hit
GTCATGGGGTTTGTGTGGGTGGGGGGGGGGGTCAGGGTAGGGGGTCACGG17.6923076923076925No Hit
GCATTGGTACTATCAAATAGTCTCAAATCACCTACTCCTCTTACGGGAAT17.6923076923076925No Hit
ACAAAACCCCTATCCGTTAGTTTTCGACTTTTCGGAAATCTATTAGCCGA17.6923076923076925No Hit
GTGAGCCAGGTAAGCAAGGAAGTAATAGAAAGACTCTCAAGTGTTATCAT17.6923076923076925No Hit
GCACGTAGAGTAATTGATCAAATACGTTGACGTTCCTATGCAGAAGCACT17.6923076923076925No Hit
GTATTTAGGTGCATGTTTGCATATCTTTCTTCCTTTATAATTATTGGAGT17.6923076923076925No Hit
GTAGAGGTTGGCATCATGATCCAAACCACCATAGTCCCCATTAAAATCAC17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)