FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAGTAGTTTTCATAAATTGACCTGAAACTTGAAGTTTTTTGTGATTG24.3478260869565215No Hit
AATGAATTCCAAGGTTTCGGCATAAGAAAAAGTGGAAAGACATCATAATG24.3478260869565215No Hit
GATTTATTCATTCCTTGTAGTTTTTTACTTTCACACGTAAACCCTATTTT24.3478260869565215No Hit
CTATTGAGTAATTGACTGGTTGGTGCGTGGGGAGAGCATAAGGTGATAGG24.3478260869565215No Hit
TTACCCATCAATCTGCCAGATTATATATCATCATCATATAAAAGTTCATA12.1739130434782608No Hit
GACTTAAACTACAATGTCCCCAGTGTAATTAAGCTTAAGGAAGGTGATAA12.1739130434782608No Hit
CTTTTTTATAATTAAAAAAAGTTTTAGCGCCTTTACATTGGTACACAAAG12.1739130434782608No Hit
GTTGGGGATAGAGGGACTTGAACCCTCACGATTTAAAAAGTCAACGGATT12.1739130434782608No Hit
ATATATAATAACTGTACTCAAAAGGACAGAAACATTACTATTATAAATCA12.1739130434782608No Hit
GTAAATACTATTGCAAATTTATGCTACCATTTCACAAAATATCTTTCTTG12.1739130434782608No Hit
ATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC12.1739130434782608No Hit
CAAAAAACTCTTACAAAATAGTAACAAAATTACATTCACTACAACTTAAA12.1739130434782608No Hit
GGCTTCAAGTATGTATTTCTAATGTGTGATTTTTACATAANAGTAGAAAT12.1739130434782608No Hit
ATGACTTTTGATAATAGAAAATCATTTATTTTTTTGTTATCTCTGCATAA12.1739130434782608No Hit
CCGCTAACCGCTTTTGGTCACAAATCTTTGGGGTTGCTTTTTCCAATAAA12.1739130434782608No Hit
AATAATTACAATTTAATTATATAATCAATTTGTATTAAAATATCAATATT12.1739130434782608No Hit
GATTTATTTCTGCAAATCGCCGCTGTGTGGTTGAGACGGCATAGGGATGA12.1739130434782608No Hit
GGAATTCCAAATGGTGTTTCATTCAGTAACAACAGTAAAGAAGTCTAGAA12.1739130434782608No Hit
ACATCATAGATTGATATATACGTTGGTGAAGAGCAAAAACTGATACTTGG12.1739130434782608No Hit
ATATTCGGATATACCCATTAATTCATTTTGAAGCCTATCTAAGATAAAGA12.1739130434782608No Hit
GCATCACTAGCTTATGCTCTGACCCGAGTAGCATGGGGCACGTGGAATCC12.1739130434782608No Hit
ATAGTGGTTTCATCCAAGTTGTATTTAATGGGAAGCTCATTTACTTAGTA12.1739130434782608No Hit
GATGAAGTGTGATCCACCATCACTAATGACTATGTGCGGCACTCCAAATC12.1739130434782608No Hit
GGGGGTGGTGAGTGCGTGTTGGCGGTTGGTTTGCTTGATTTGTCGGGTAG12.1739130434782608No Hit
ATATTAAATTATTTTTATAGGGCTGACGAAAAAACGGACCCGATATCCAA12.1739130434782608No Hit
GAGATAGGAGATTGACCGAATTGTATGAACCACCCTGTCAATGATTTGCG12.1739130434782608No Hit
CTTCTACACTCTTGCTCGTCGGATCATCAGCTATAACATATGTCTCTTTT12.1739130434782608No Hit
TCGGAGAACTGAGCCTTAGACGGAGATGGTCCGACTGTTATCATCAACTC12.1739130434782608No Hit
AATTGACTCACCAGGAGTGTCTATTTNAGGAAGTACTCTAACACCATGCT12.1739130434782608No Hit
AAGACAACAATGATTCCTTGAGCACTTTGAGAAGTAAACAAGGTGTGATC12.1739130434782608No Hit
ATGTTGACATAGTGATCTTTGTGAGTTATTGACACTGGCCTCACTCTCTC12.1739130434782608No Hit
ATATATAAGGGATCCCTTGGTTCGGAGCGAAAAAGCAATGTCACTCGATC12.1739130434782608No Hit
CATACAAAGAAACATTCAGAATTGTAACAAGAGTCATAATAGACCTTAAG12.1739130434782608No Hit
TCTTGAGGCTAAGTATTTCTTGACCTCTCCTGATAGATCTCCACGCTTTC12.1739130434782608No Hit
CTATAAGGGGCTCGTACATGTTCGGCAGTACCTCCTGTAAATAGCCCGCC12.1739130434782608No Hit
CCCTAAAACGGACTATGTAATGTACTTTATCTGCTGGGTTACGGGGGGCT12.1739130434782608No Hit
ATATTAGATATCTCATAAGAATTCAAAGAATTAGTTGGGGACTACAGAAT12.1739130434782608No Hit
GAGCGTGGGAGACGTTTTTGGTGTTTTTGGTGCTCTACACAGCTTGGGCA12.1739130434782608No Hit
GAATCGAAACGATAGGGCTTGACGATGCTGCTTCGAAATTAGGTTATTTC12.1739130434782608No Hit
GGCTTCAAGTATGTATTTCTAATGTGTGATTTTTACATAAAAGTAGAAAT12.1739130434782608No Hit
ATTACATCCTTTGCANGTGTCGAAAGAAGCGTACCTGAGAAGTAAATGGA12.1739130434782608No Hit
GGTATTTTTAANCCCAAAAAAACGCGGCTAATACGTATAAACATCTTTCT12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)