FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAATTGAAAACCTGGCAGCAAGCGCTCTAACAGATCTTCTGACTAT14.545454545454546No Hit
AACAAATTCTTAGTTTCTTTTTTTCCTTTTTTTACTTATGTATTAATTTG14.545454545454546No Hit
CATCTGAGGAAAAAGAGAATTCTGGTACGTTTGTCACCTAAAGAAGTCTG14.545454545454546No Hit
AATATGACTTGATCTCATGTGTATGATTGAGTATAAGAACTTAAACCGCA14.545454545454546No Hit
CTTGCATCCATCCTGTGAAGAACAGTAAATTTTGATTGCAATTGAGATGT14.545454545454546No Hit
AAAATGAGGTAAGGTGTTTTNGGTCGATCAATGTAATCGTTTAAAAATGG14.545454545454546No Hit
CCAGTGGCTAATCGTGGCGGTGTGATGCCGCTCGCTCCTCCGGTTGGTTG14.545454545454546No Hit
GAGTCCTATTGCTGCGGAGCCTTGTTTCACAGCTTCCATGGCGTATTCGA14.545454545454546No Hit
GGGGGGGGGGCGGGGGGCGGGGGGCGGGGGGGCGGGGGGGGGGGGGGGGG14.545454545454546No Hit
GTACCAAAAGATCCATTCAAACTTAAGAAATTTGAAACAAAAAAAAGTTC14.545454545454546No Hit
CATTCATAGGACAGTATATGCTTTGTTTTGCTCTGGTTTTTAAAATAGTT14.545454545454546No Hit
GATATGATTAGTCTATCATATCTTGACTGATTTTTTTGGATCCAGATAAT14.545454545454546No Hit
ATTTATGGTCGTACAAGTAGGCTGTGTAGAGTTGGAGCGACCTGGCAGTC14.545454545454546No Hit
ATACAAGGCCACGCAATTTTCCACCAAGCATACAACTACCTCTCTTCCTC14.545454545454546No Hit
ACATTAAAAAAAAAAAAACAAAGATAAAAAATAGAACAAAATTCTACAAA14.545454545454546No Hit
GGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGG14.545454545454546No Hit
AAATTGATTAATTAAACAAAGATTTATATATATAAGATTATAACTACAAT14.545454545454546No Hit
CCCTATTAGGATGTACTATTAAACCAAAATTGGGGTTATCCGCGAAGAAC14.545454545454546No Hit
TAAATACATTTTGGAAATAATCAACTTCAAAAAGAGAAACATAAAAAGCA14.545454545454546No Hit
GAATTAATATTTTATTCTATAGTATTTCGTTAATTGTGGATCCTTAATTA14.545454545454546No Hit
ATTCAACAGGCTCTGGAAGGACTTCCGGGAGGTCCCTATGAGAATTTAGA14.545454545454546No Hit
CTTCCGAGGCGTTTCGAGGTCGCGGCCAATCCTTAATGGATTTAATCTTC14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)