FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCAAGTTCTGAAAGGCTGCGGGAAAGATGAGTTGCTAAAGGTAAACC27.4074074074074066No Hit
AAATTGGGCTGCTTGGAGGCCAAATCGAAGAGACGGAGTGCTGCGGAATC27.4074074074074066No Hit
CTATTTCTTAGATTCATGGACCCAATTCAATTCAGTGGGATCTTTCATTC27.4074074074074066No Hit
GACTAGAAACGACATCTCTTATGTCAATGACACCCAAGGGATATTAAATG13.7037037037037033No Hit
AAGTGTGACCTCGAAGACAGATTGTGTATAAACTTGAAATTTTTTTGGGG13.7037037037037033No Hit
AGGTATGTGTTGCGCCTCATTGCTAGCACCATTTTGTGCCGGTGTTATCC13.7037037037037033No Hit
TCCCACATTCTGCAACAATATCCAAAGCATACTTTCGTTGAGATAAAAAA13.7037037037037033No Hit
GGCCCAATAGTCATCGTGACCCATTATAAAATCCATAATCAGAGAGCTTC13.7037037037037033No Hit
CGATTAACCTCGGGATTTGAAATCACTGCTTATATACCTGGTATTGGCCA13.7037037037037033No Hit
CCTATGTTGTGTTGAAGGGATATCTAGATGATCCGATCGATTGCGTAAAG13.7037037037037033No Hit
CTAGTTAATAGAATCAAACTTACATTTCTTGAAAAAAAAAATGTTGCGGA13.7037037037037033No Hit
AACTAACAGCTCGTGTTTTCATCAAGATGGCTCCCATTCTGTCTCAAGAC13.7037037037037033No Hit
ATGCTTCCCTTGTGTCGGTTGACAACCCCTAGACTTGAATGACGCCTACG13.7037037037037033No Hit
ATTCTACAACATAGCAAAACGTGTTCTTCGTTGAGAAGGAGGGTTAATCG13.7037037037037033No Hit
TAATAATAGAGTAATATAGATATAGAGAACTTTGCAATATATATATATAT13.7037037037037033No Hit
GCTCTAACTCGATTATTTCCTAATAATTGCTGTACTTCACAAGTCACATT13.7037037037037033No Hit
TCACAAAGAGGCAAGTTGTTGGAAGAAACAGAACAAAGGTGTTCAACAAA13.7037037037037033No Hit
GATTTGACACTCAGTCGAGATCCGGACAATCAGATATGTTCCCTTTTTTA13.7037037037037033No Hit
TCCCGAAGCAGATCAGTCAGTCAAACGAATCTATAAGGTGGCACGGATCA13.7037037037037033No Hit
GCTTATCCCCCACCGTCTCACTGGCCGACCTTGACCCCTGTTATTTTGAG13.7037037037037033No Hit
ATCTATTTAGTGTAGTCTTCCCTTTTCGCTCGTTGATTGGTGGTAGTGGT13.7037037037037033No Hit
TAAAAATAAAATAAATCGTTGTAACTAAAAAGATCAATAAAAAAATTAAA13.7037037037037033No Hit
TCTTTATTCGTCCTTATTTTTGTAGATTGAGCATAATTTGATGGATAGGT13.7037037037037033No Hit
ATTCAGAAAGTTACCCGGCTTCAGAAATTATGGTACTCTTCTTTTTGTTC13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)