FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n02_ath_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n02_ath_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATGGTGGTTGGCAAACAAGGTCAGCTGGACCTTGTAATATNATCAT34.225352112676056No Hit
TTATTGAAAAAATCCTTGTTCGCTTGAGCACCGATTTTTGGCCATCAAAA22.8169014084507045No Hit
GCCCCACCATGCAAGCTTTGGGAATTTACAGAGAGGTGGGAAAGCTTTAT22.8169014084507045No Hit
GTCGGGATCATACCTCTATTCCCGAACTGAGATTTTATATTTTTCATAAG22.8169014084507045No Hit
GGTTACGGGGATTATCAAGTTTGATCTGCATGCGGAAGCTGAGACAGACA22.8169014084507045No Hit
GCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTC22.8169014084507045No Hit
TAACAATACAATGAGTTTGTGTTTTGACTTTTTTCATACCTTCTATCGGT22.8169014084507045No Hit
GGTTTAACCGATTTATTATTTCTAGAATATCAAGTCACATAAGGTGCCTT22.8169014084507045No Hit
CTTAAAATATTAGACCGTCTCCTACTTAGCTCAATTCAGTTCGGATTTAT22.8169014084507045No Hit
GTTTTGCTCTGGTTTTTCCAACAACAACCGAGTCCGCAGCTTCCAAGTGA11.4084507042253522No Hit
GAATTTTAACAGCTTTAGGAAACAGTTACCACAAAACCTCATAGCCTCAG11.4084507042253522No Hit
TATCTATCCAACTCTCGCCGCGACGACATCTATCTTCTAAACAGAAGCAA11.4084507042253522No Hit
ACCATATTTTTAACAACTGTTAAGCATTCCATGAAGATCCGGGCTAAGTG11.4084507042253522No Hit
TCACTGAACCATACAAAACAAAATTGCATGTATAAAAGAGGATAAACAAC11.4084507042253522No Hit
ACAATACAAGGCCAATAAAAAGAAGAAAACCAATTTGGTGAGTCTTCAGA11.4084507042253522No Hit
TGTTTAGAACAGCAGTATTCAGACACCATTTGGAAATAAATAAAGGAATG11.4084507042253522No Hit
GTTTGATTTATCTTCCGATCAAAAACTTTATTTCTTAAAAGGATTTAGTC11.4084507042253522No Hit
GGTTTTCAATGAGTTGTGTCCATAGCCATTTGATACTTCAGTTCAACTCC11.4084507042253522No Hit
CATCTATGTCCATGAGCCTGTCATCAAACTGAAGATTTGGTATGATTTTA11.4084507042253522No Hit
TATCTAAATAGATACTATGTTTACATAGGGATCCCTACGTCGTTACATTC11.4084507042253522No Hit
GGAACCCTTGGGTTTTCGGGGCATTGGATTCTCACCAATGTTTGCGTTAC11.4084507042253522No Hit
ATATATAATTGGTAACTCGTACGTATATATATTGCATTTGTACTTATTTA11.4084507042253522No Hit
GGGTTGGTCGCACGAGAAGATCCTTCCATATACCTTTTCCGGCGCTTTTC11.4084507042253522No Hit
GATTTGTCCATTAGAGTTCACTAGGTTAGTAACCTTTTCCTCCTCCTCTT11.4084507042253522No Hit
GAGTTATGCGAGACATGGTGCATCTCTCAATATAGATGAAAAGATCATCA11.4084507042253522No Hit
CATGGAAACTAAGCTCGATCGGATCTTCAAGGGAGTCTGAATTTAAGAGC11.4084507042253522No Hit
CTGTTGTGTACAAAGACGAAGATTCTCATGTTAGGCTTTTATCAAGTTGT11.4084507042253522No Hit
AGACTTTCCAGCCTATGGCCGCTTCTTTATGGTAGCAGTTGTTGTTGCAT11.4084507042253522No Hit
CTATTACCACTCCCCACCCCCACCGGTGAAATCACCACCACCACCATACT11.4084507042253522No Hit
ATCTCAATACAAGGACACTTGCATTGTATTACTAACCTAGCAAAAGTCTA11.4084507042253522No Hit
AGCTCAAGCTTTAAGGGACAGCAGCATGGCTGGTTACATGTCGAGCAAGA11.4084507042253522No Hit
AAGGACAATAATATGGAACTTGCAAACCTGGAAAAGATGAAGATACTTGA11.4084507042253522No Hit
CCTTTAACCTAATTTCTCACGCCTCTCCAAAAAGGTTTCACCTTTCTCCC11.4084507042253522No Hit
ATTTCTTAGTCCGAAACACAAACAAGACAAGATGACGATAAAGGCATGTC11.4084507042253522No Hit
GTTTTTAGTTGTTGGAAATCTTGCTGCATGGTTGTCAGAGTTGCGGTCAG11.4084507042253522No Hit
TTCTCAAGCCAATAGTTTTCCCAAAAAAAACCTTGGTGTATGGACCAATA11.4084507042253522No Hit
CTGCAAAACCTGATCCGTCTGTGGTAAAATTATGTTTTGCTTACTTGTTT11.4084507042253522No Hit
AGATGCAGCAATGGATGATGATACAGAGATGCTAAAGATAGAGAGTGAAG11.4084507042253522No Hit
GATTAGCGTCGAGCTTGGTTCCTCATCAATGGCGTCTCTTAACATCTCAA11.4084507042253522No Hit
CCTTTTTGTCTCGAGATCGACCGATCCCTTCCAAGATTGATTGATCCCAG11.4084507042253522No Hit
GTCATGTGTGTAATATATAAAAATTTCTTAATCAGGTAAAGATTCTAGAC11.4084507042253522No Hit
GAAGAATGTTGATGACTCTGAGAAGAATAATAAATATTAAACGGATGCTA11.4084507042253522No Hit
ATCATGGTATGCACTTCCGTGTACTAGCTAAAGCTTTACGTCTATCTGGT11.4084507042253522No Hit
TAGCCGCAAAGGCTCCGTATGACAGGGAAAATAAGACACCGAATTACCGA11.4084507042253522No Hit
CTTTTTCACCTCAAACCTTTTTGCGATTTTTGCAATTTCTAAAACTCAAA11.4084507042253522No Hit
GCATGAATGCATTCACTCCAGTAAACTAATGGCAGATTCGATTGAAAGAG11.4084507042253522No Hit
CTTTTATAATATAGTACAGTTTTTATAATAATTACTTAATATCATGTCCT11.4084507042253522No Hit
TTCTAGAACGAAGGGCCACGTCTCTAAACACAAAAGCTTTATCACCATTA11.4084507042253522No Hit
CAACTAGAGACCCTATTTATAATTACTAAAAGATAACCACAGAGCTACGT11.4084507042253522No Hit
GTAATCTCCACCATCCCAGCCTCTACAAGGCGTGTGCGTGAGCCATTGGT11.4084507042253522No Hit
GATATATTCAGNAATTTGACATAGACCCAAGATATTTTATCTTGACATGT11.4084507042253522No Hit
ATGCTACCTTAGCTGATTTGGCCAACAAGCTCACATATTGCAAAAATGAG11.4084507042253522No Hit
TACATATTCCATACTCATAANTTCAAACTAAGATCTATCTGATATAACAC11.4084507042253522No Hit
GTATTGCACGTTCATTGGAGGTAACTTGGTCACATGGAGATCCAAGAAGC11.4084507042253522No Hit
AAATAACATTAGGGGGGGCGATTGGTTAAAACGTTACTATAGACTTTCGC11.4084507042253522No Hit
GATTAGATGGAAGCACCGGAAGAGACTTGAGCCTGCGGCAATGTTTCAGG11.4084507042253522No Hit
GCCACATCTCTTAACCCTACTCACCACTTCGTTAGTGGTTCGCTTATAAT11.4084507042253522No Hit
ATATAATGTATCGTTTAGCACATCAAGACGACAAAGAAGCCAACATACTC11.4084507042253522No Hit
TATTACCAGTANTCGATACTGGGTCATTCATAGCATTACTATACCTTCAC11.4084507042253522No Hit
CATCCTCAATCCGTGAAGAGCATTGGAGAAGTCTCTATCTCCAGAGATAA11.4084507042253522No Hit
ATATTGAGGTCATTCATCAACATTACCTTGGCCTCTTGTGACCAACTATA11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)