FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_chi_092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_chi_092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGAAGAATAACTCCAGAAAGAAATGCACAAAGCGCAAATGATTTAT17.142857142857142No Hit
TACAAAAAAAATTAATAAGAATCCAAAATTTCAAAAAAAAAATTACTAAA17.142857142857142No Hit
CTGCTTCTCCAGCGCGATGCTTACGATCAGAGGCCCCGTGCGCACCTGCA17.142857142857142No Hit
ATCCATAAATATATTAAGAAACTGAACTTACTTTGATGAATCATCAAATA17.142857142857142No Hit
CTATATCACAAATAAATTTTACAAATATAATAAACGAACAAACATTAAAT17.142857142857142No Hit
GAATACGCCCATACTTACTTACTATGGAAAACTGCAGCAAGTAATTCAGA17.142857142857142No Hit
ATTCTCTGGAGCTATCATATACTCCATTGAAAAGCTTGCTCCTAACAAAC17.142857142857142No Hit
TAAGATGGTTATGNATCGAAACATTAGATTAAGATAAACGAGCGACAAAA17.142857142857142No Hit
TGATCACGACGGTTCCTCCGAGGTATGTACGGTCTGATCAAGACGTAGCT17.142857142857142No Hit
TAATAAAGGACTATTATCATTTTTATATACTGAATTCCACAAATAGAGTA17.142857142857142No Hit
ATGCTTTTAGAGCGAGCAGTCACTTTACCTCCATTTCCAAGCAGTTCATT17.142857142857142No Hit
CATGTTATCTATCTCTTGACTCGAAATGGGAGCAGGTTTGAAAAAGGATC17.142857142857142No Hit
CATAATGTTCGCTAACCATTCTAAGCCAAACAATCGCATATTCCTTTATC17.142857142857142No Hit
CATTATTACAGGCAAATGGGATTCGGGATAAGATTTAATTATGAAAATTG17.142857142857142No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)