FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_chi_076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_chi_076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCAGGGTTTAGGGGTTAGGGATTAGATTTTAGGTTTGGGATTTAGGG18.333333333333332No Hit
GACCATAAACCTCGTATTGATTAAATGATTTGGAAATATATGTAATATGA18.333333333333332No Hit
TACCAATAGTGTCGACAAATTTTTGTATTTCAAATGATATGTTTTCTAGA18.333333333333332No Hit
GATCAGATGAGAGTTGTAGATTCTGATAAACCCGTTGTCGCCTTAGGTAC18.333333333333332No Hit
ATTGTTGACTGGTACTTTTGTACATTCGTGTCTTTTCATCGTAAACAGTT18.333333333333332No Hit
CAAAATGGGAATGGACAAAGGTTATGAACTTATAAAATATAATCTGATGA18.333333333333332No Hit
CAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTT18.333333333333332No Hit
GTACTGGAAGGTGCGGCTGGATCACCTCCTTTTCAGGGAGAGCTAATGCT18.333333333333332No Hit
TTATTAATCACCCCCTTCTAATTAGAAACGTCATAGTGTTCCTATCTTTC18.333333333333332No Hit
GCATAGCAACACCTTCGAGTTCCTTGAGCTTCTNCTCGTACTCTTCCTTG18.333333333333332No Hit
CATCCCGACCACGGGATGTTGGGCTTCAACTTCCCTTTGGCCTTTAGTAT18.333333333333332No Hit
GGTAAGAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCGAGTACCAGGC18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)