FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_chi_067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_chi_067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTATGACATCAAGTCATATTCGACTCCAAAACACTAACCAAGCTTCT31.744186046511628No Hit
CCTTCCTCTAAATGATAAGGTTTAGTGGACTTCTCGCGACGTCGCGGGCG21.1627906976744187No Hit
TCAAAAGAGTGGCTCTTGTTAAAGACAAGGAAGGAGATAATCAGGTTCTT21.1627906976744187No Hit
GTATCGATCTTATCGATCTATACCAAGTTTTTTTTTTCTTCGTACGGTCT21.1627906976744187No Hit
CCGCCGCACAATCACATTCCAAGATACATCACTAGGGTTTACAATCTCAT21.1627906976744187No Hit
GTCTACAACCATTATGTGGCATAGGGGTTACATCTCGTACGAAACTTAAA21.1627906976744187No Hit
GTGGTGAAAGAGCATGAAAGGTAGATAGATAGAGATAAGTGATTGAAGGG21.1627906976744187No Hit
GATTTAGAAGAGAAAGAGAAGTAAGGCCTACCATCAATTGCCCAAACCGG21.1627906976744187No Hit
TGCTATTGGTTTGTACATTATTGCTTTCAAAGCATGTGGATTGTTGGCTT21.1627906976744187No Hit
GATGATTCTCTGTGTATTTTAACAGCTATTATGCAAGTACGAACCGAAGT21.1627906976744187No Hit
TAATCTATCTCTAACTCTTATAATTAGTCAAAGGCTTATAGGATTGTGAC21.1627906976744187No Hit
TGCTATAACTGCAGGATAGCTTTTTCTTCAGCTTGGCCTAGCTTCACCTT21.1627906976744187No Hit
ATGCCCATGACTGCCACCACCACTACAACTACTAGCGGAACCACTAGTAA21.1627906976744187No Hit
GTATAATGTACTGGAGTCTTCAGAGACATTCACGGGAGAGTTCAGATTCG10.5813953488372093No Hit
CTCCGATACAGCTTTCATGTAACGGATCATTCGATTTGGCGGGAGCGCAA10.5813953488372093No Hit
TTCTTAAATCGAAATAAGGAGTCCCAATCTTTGTTAATTTTATCATCATC10.5813953488372093No Hit
TGGCCGGCTTTCGAACAGCCGGACTCCCATCAAAAGATGGTTGCCAAGAA10.5813953488372093No Hit
GTGTTTAGAGAGAAAGTGTGAAACCTAGTTTTATGGTTTTGGATCTTATG10.5813953488372093No Hit
GAAGTTGCCCGAATATGGGAGAATGCTGGGGCGCCGGAACGGCGACCTTC10.5813953488372093No Hit
GTATTACGAATACAAGCTGTATGGGAAAAATATGATGAGTGGGGTTGATT10.5813953488372093No Hit
CTCGGACCTGGTCGACGATTTCAACCCCTGGTCGACAAATCCTCGGACCC10.5813953488372093No Hit
GGATAAGTTGGTCGTGAAACACTCGGATCTTAGGCCTTTTAAGGTGTGGG10.5813953488372093No Hit
GACTTAAAGGGAGCAGTTCTGAGAATATTAACCAAATTCCCTTTCCAAAA10.5813953488372093No Hit
CTAGAACAGTTTGGAATAGAAACTTCCCGTAAGCAATTCGTTCATCATGG10.5813953488372093No Hit
GTATCGATCTTATCGATCTATACCAAGTTTTTTTTTTCTGCGTACGGTCG10.5813953488372093No Hit
GTAGTCAGTGTGATCGTGAGTGTTGAAGGCCGATGTTGCAGAAAATTTTA10.5813953488372093No Hit
CATCTTAGGGTGATCCTTTTGTCGACGGATGCTCCTATTACACTCGTAGT10.5813953488372093No Hit
CCCCCAGGTCGAGTGACTTTGGAGCCATTCTTCCCATACTTTGGAGCCTG10.5813953488372093No Hit
GAACCTAATCCTGAATATGAACCATAAAAGAAAATACCTATTAAACCGAT10.5813953488372093No Hit
ACATCTAGTTGTAGTTGATTTTGAAAGATGAATAAGTCCATTTATTTAGT10.5813953488372093No Hit
AGACTGAAGATCATCATCTCCTTGAAACACTGCTTTATGACATTCGGTTT10.5813953488372093No Hit
GCTTTGTATCGGCTAAGTTCACGAGTTGGAGATAAGCGGACTCGAACCGC10.5813953488372093No Hit
CTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCT10.5813953488372093No Hit
CCACAATGCTTTTTTAAGTTAGCTCCATAACTTTTGCTGTCTTCATGATG10.5813953488372093No Hit
ACTGTAAGCAAAGAATACGTTAGCCAAACTTCTAGAAACAGATATAATTT10.5813953488372093No Hit
AGAAGATTGGTCCGCGGCAATATCGTATGGTCTAGGGTTTCGGTTGATTA10.5813953488372093No Hit
ATACTCAATCATACACATGACAACAAGTCATATTGGACTCCAAAACACTA10.5813953488372093No Hit
ATCAAAGAAAGGAACAAGTGGATTTAGAGTTAGTGTGAAGAAGGGCGGGG10.5813953488372093No Hit
CTCAATCTCAATCCCCAAATAAGTTCCCACCTAGTCCTACAAAGTCCGAA10.5813953488372093No Hit
ATCATACATAAGACATCAAGTCATATTCGACTCCAAAACACTAACCAACC10.5813953488372093No Hit
CTGATAAACATAAAAGAAAAATTGATTTGTGTTCTGAAGTGTGAATGCTA10.5813953488372093No Hit
CATTATCAAGTTCATAATCATTAAAGTCAAATTAGGACAGCCTGGCCCAA10.5813953488372093No Hit
GTGGTTACGCTTCTGATGTAGCGGCTCGGGTTTTTGGCATGAGAGGCCGG10.5813953488372093No Hit
TCCATACATAGTGTTTTGATCTAAGATTTCAATTCTTCCATGTTTCAGTA10.5813953488372093No Hit
ACACTCGATCCAATTGCTGCATAAAATTATTAACTATGGTTTTGATTTTT10.5813953488372093No Hit
GTTCAAAATGTGTACCATCTTGCTTGTCATAACAATGTGCCGGGTATCGA10.5813953488372093No Hit
AGTATAAGTGATCAAGGGGTGGTAACTCATATTACTGGAGGAAACTTTGC10.5813953488372093No Hit
GGTCAAAATGTATCACTATCCAATACATTCAACTAATTATAAGAGTTAGA10.5813953488372093No Hit
ATATAGAATAATAAATAATAAAATTTACAGCTTTTACAACTTTCTGTTGC10.5813953488372093No Hit
GGACGTGGTGATGAATCTTGAGGTGTTCCAACATTAGATTCGTTGTAAAA10.5813953488372093No Hit
CTCAAGGATCATGCGATGTATATAAATATGATTTGGGGTTGGGACCAAAT10.5813953488372093No Hit
ATGCTAACCCAGATTCTTCTTGCTCTTCAAAGCTTTGATGGTGTAGCCTA10.5813953488372093No Hit
CTCTTAATTCCTTCGCTTTCCAAAAAAGGTAATCTAAGTTCACAAACAGA10.5813953488372093No Hit
GACTTACTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAG10.5813953488372093No Hit
ATGATATTCTATATATTACGTTGCAGTTAAATATATCGGGTAATTACTCG10.5813953488372093No Hit
ATCCATACTAGATCTGACCAACTGACCATCCTACATCCTCTACGTTCTTN10.5813953488372093No Hit
ACGACACGTGCCTTTGGGAGCCTAAGCTCCTACTGAGGGTCGGCAATGGG10.5813953488372093No Hit
AATTTGAATAGATAATAACAAATTGTAAAATTTTATTTTTGAAATAAACT10.5813953488372093No Hit
ACGACACGTGCCTTTGGGAGCCTAAGCTCCTACTGAGGGTCGGCAATCGG10.5813953488372093No Hit
CTCATACATATTGATTGAAACAAGCAACATATTTATATAGAAAACATAAA10.5813953488372093No Hit
GGACAATAAAAAAGCACGCGATTTCAAACCTAAATAGAAATGTTTTCGAA10.5813953488372093No Hit
ATCAAGATGTGCCTGGTTAACTGTCCAACATAAGAACACAAGGAAAATGT10.5813953488372093No Hit
GTATATGTGTTTAGGCAAACCGAGTTCGATTAGAGTTTAAGAAAGGTGCG10.5813953488372093No Hit
GAAATACTCTGATTCGAGCAGTGGCATCTTGACCGGATTGAAGAAGAACA10.5813953488372093No Hit
TTCTTATACTCAATCATACACATGAGATCAAGTCATATTCGACTCCAAAA10.5813953488372093No Hit
TACTAAGCCTGAGCAGAGAACCTAATTCTGTTTACACTTTTTGTCGTTTC10.5813953488372093No Hit
CTTCTAAATGTTTTAAACGAGAAGCACGGAACTTGACTGCTTCAAGCTTC10.5813953488372093No Hit
GATCTTAAACGCGTAAAAATTGTATCCTTGTTAGAAGACACAAAGCCAAA10.5813953488372093No Hit
CTTTTAGTTATGCTGAATTGTGTTTTTTTTTTAGTTACGAAATGCAATTC10.5813953488372093No Hit
CCTTCATCAGCGGCGCCTTCAGCGCATCGGCCAGCGCGTTGGAATCGGGC10.5813953488372093No Hit
ATGAACATCTTTAGATTGTATGTAAAACTATTAGAAGTTTCGGCATTTTG10.5813953488372093No Hit
CAACTCTAGCATCTGATCAATAACCATCCGACATTGAGTTAAGGGGGTCG10.5813953488372093No Hit
GTGTACCCGTGCTGGGCTGGACGCCTAAAGCCTCCGGGCAGATAAAAGAA10.5813953488372093No Hit
CCTTATTCATCTATAACTCTTATAATTAGCAAATGCTTTGGATTGTGATA10.5813953488372093No Hit
TAATATGAGTTTCGACTTACCTTGTTCTTTGCCAGGAGCCTGCTTCTGTT10.5813953488372093No Hit
GTATATGTTTTGTTCATATACAAAAAATCAACTCCTACATCCTTTAAAAA10.5813953488372093No Hit
GGCATATGCTGACCTAATTCAACATGGTAAAAAGAGGCCTCGAGGACAGT10.5813953488372093No Hit
GTGCTACTGAAGAAGAGATGGCATCACATGAGAAGAATCAGACTTGGAAA10.5813953488372093No Hit
ATCCAAAATGAAAAGAAATATTTTTAGCTTTAACCAAAGAATAGACGAAA10.5813953488372093No Hit
CTGAATCCCAGCTCAGACATATCTTGCTTTGCCATCAAATTCCTGCATGA10.5813953488372093No Hit
GAACAGGCGAATACAAAAGGAATTCAAGTACAAATTGCAGGACGTATCGA10.5813953488372093No Hit
GTTCAGGATGCCGGCGAGCTCGTCGAGGGTGACCCCCTGCGGGTGGGCGG10.5813953488372093No Hit
GATAAAAGCACGCTCCAGAAAATTAGAGACAAATATTACTATTCAAAAAG10.5813953488372093No Hit
ACACTATATTTTGATTCTGCCCTTCTAAACGGGACGTCAGCTCTAACAAT10.5813953488372093No Hit
ATCATACTAGAGACAAACTAATGAACTTCACAGGTGGATGTCAACGAATC10.5813953488372093No Hit
GAATATAGAACAAGGTGCAATAAATAGATAGACTACATCTTCTTATCACT10.5813953488372093No Hit
TAACTGCGGAGTTCTGATGGGATCCGGTGCATTAGTGCTGGTATGATCGC10.5813953488372093No Hit
CAAACATTATTGTTCAAAGTTCAAGTGAAAAGGGACTGAAATCTAAACCT10.5813953488372093No Hit
TATAATCACAGTATGACAAGATGTTTTGCTTTTGATCCTCTTTACTCAGT10.5813953488372093No Hit
GGAGCTTATCGACAGAAAAAAATCAATTTAGATAGTCCTTTGTGGCTTCG10.5813953488372093No Hit
AATATCTACAGCCAAAGCACATATCTATTTTTATTTCCAAGCAAACTGCT10.5813953488372093No Hit
GCTGAGAGTCTACAATGTCACGAAATACATACCACAGTTGCCAAACAAGG10.5813953488372093No Hit
TGTGAGAGCTAACCTCAATAGAGCCATGAACAACTCTCCCATATGCACAA10.5813953488372093No Hit
GTCCAAAGGTTTGATCTCACCCACAAAACTATTAGATATTCGTAGAAGCT10.5813953488372093No Hit
TGATAGGATTCTGCTCTCAAATATTTTGTTAATGTTCCTCCAACGGGTTT10.5813953488372093No Hit
ATGTCTCTCTTTATTTTTTTAATGTTTTTGATACATTTTCGTTTATGCTT10.5813953488372093No Hit
ATCTCCAAACATTTACAGAGCCATATTTACGCAGACACTCATCATAAAAT10.5813953488372093No Hit
TGTAAGTCTTTCAGCATTTGAGCTAACCACAACAATTCCTTAGTCGCGAC10.5813953488372093No Hit
GGCAGCAACGTTGGACCAAGGCATTGATTGGACCAATGCATCGAATAAGA10.5813953488372093No Hit
AGGAAAAACCCTTTATAATACGCTTCGCTGAATTTCTTTCCACCAACCGA10.5813953488372093No Hit
AAATAGATGGCACAAGTCATTCATCACAAAAGAGTAGCTAGACCTAGAGA10.5813953488372093No Hit
TTCAAGTGCTGTTGGAAGTGTGATGTACGCAATGATTGGTACAAGACCAG10.5813953488372093No Hit
ATCAAGAGATGATTAGCAGTGCCTCCACTCAAGAAACATACAAGAAGAAA10.5813953488372093No Hit
ACCTTACCTAGATGCGTTTTAGGCCCAATAGATGACGCCTGAGTCACTCT10.5813953488372093No Hit
GTATGATTGAGTATAAGAACTTAAACCGCAACCGGATCTTAAAGGCGTAA10.5813953488372093No Hit
GTAGTGTATCCTTGTTAGAAGACACAAAGGCAAAGACTCATATGGACTTT10.5813953488372093No Hit
CCATAACACAGTAGTAACAGAAAACCACACAAAACCTGAGCAAGGAATTC10.5813953488372093No Hit
AAGCTTGTCTCGGTTTAGTGAGGTTGAGTGAAGTATATGTGATCAATTAA10.5813953488372093No Hit
ATGTTGCACAATCAAATCAACTGCAACGTGATTGTCACCTCCTCGTGGAA10.5813953488372093No Hit
ATTCTTTATAACGTACTCTATTTTTTTTTGACAAATAAGCCAGCAGTCGT10.5813953488372093No Hit
GTTCCAAATCCCCCTTTCCCAAGAACATTCTCAAATGATTTTGTCATCTT10.5813953488372093No Hit
ACGGCATTCTGTGCAATCTCCCAAAGCCGGCGTTCGCCGCCGGGCTGTTA10.5813953488372093No Hit
GTATTAGATGTTGCACTTGTGTCCTAGGCCTTCCATTCCGGGGCTTTAGT10.5813953488372093No Hit
AAACATGTTCGTAAGGAAAAAGATGCAAAATTTAGAGGTACAATTTTTGA10.5813953488372093No Hit
AATATGACTTGATGTTATGTGTATGATTGAGTATAACAACTTAAACCGTA10.5813953488372093No Hit
AAGATGGAGAGTCTGTGGATAGTGAAGAATGGGAGCCATATGATACGAAT10.5813953488372093No Hit
TTTGAAAAACCTCTTCCTTATCTCATACTACTCTTTCAATATATAATCGA10.5813953488372093No Hit
GATATCTGCAATATTATAACATGAGTTATTTGACCTCTTAAATTAATTTT10.5813953488372093No Hit
GTCTAGTGGCCACTCTCTTTTTATTTTAATTTGTTACTTTTATTTACCAT10.5813953488372093No Hit
ATCTCTAGAGTCAATAATTTTATATGAGGAACTACTGAACTCAATCACTT10.5813953488372093No Hit
CTCTAACTCTTATAATTAGTCAAAGGCTTTGGATTGTGATACATTTTGAC10.5813953488372093No Hit
ATTATTTTGTTGTCTTCATTAATTAGTTTCTAATGATTTCTCCACATCCA10.5813953488372093No Hit
ACACAAAGCCAAAGACTCATATGGCCTTTGGCTACACCATGAAAACGTTG10.5813953488372093No Hit
GTGTATGATTGAGTATAACAACTTAAACCGCAACCCGATCTAAAAAGCCT10.5813953488372093No Hit
TGTTTAGGCAAATTACTTCACGGGCTATACTACTAAATTATCATATCATA10.5813953488372093No Hit
GCTAGTTACTTTTGTTAAATTATTTGATATTTTAGTATCCTTTTTTTATA10.5813953488372093No Hit
CCGCTATTTCAGAACCGTACATGAGGTCTTGGCCTCATACGGCTCCTCGG10.5813953488372093No Hit
ACTCAATCATACACATGACATCAAGTCATATTTGACTCCAAAACACTAAC10.5813953488372093No Hit
CACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAA10.5813953488372093No Hit
GTCTTTTTTCTATCAATCAAATTTTTGATTTTTTTTTCGCTAAGTGAAGA10.5813953488372093No Hit
GAACTTACTCAAAATTTTACCTTTTTATCCTATAGGTATACCTATACAAA10.5813953488372093No Hit
ATACTTGAGATGGCAATCCATAAAAAAATACCGATATTGAGATCCGCTAA10.5813953488372093No Hit
CAATTAGGCCCATATAATAATTTTCTTGTGTTTTTGTTTTCCCAACAGTA10.5813953488372093No Hit
GAGTACTTGATGTTACATACTCTATTCCGGATGATATGGGAGGTGAGAAT10.5813953488372093No Hit
CAAGTCCTTCAATGAAATCACTACGTCATCCTCATCACCCAAGAGCTGCA10.5813953488372093No Hit
CAAAATATCTCAAAATATATAAAAAGAGTACTGCTTGTTAAACAGAGGTA10.5813953488372093No Hit
CCACCAAACAAACATGAATGAGAGAGAAGACGAAGAAGAAGAAGAGATAC10.5813953488372093No Hit
ATTCAAAAGTGGCAGAGGAAATGAATCTCTTTGTCAAGTTGATGTGACCT10.5813953488372093No Hit
AATATCTACAGCCAAAGCACATATCTATTTTTATTTCCAAGAAAACTGCT10.5813953488372093No Hit
GAGCCAAGTGGAGCATGCATGAAGGAGTCAGATCCTTCTTATATTCTTTT10.5813953488372093No Hit
TAAACATAAACTGCACTCGTTTCTTTAGGGGCCAAAGCAAATGTTTCATC10.5813953488372093No Hit
GATGCGATAAAGGCGAATTCGTTGGACCATGGAACCAAGTCGTAGCTGCC10.5813953488372093No Hit
GTCTTTGGCTTTGTATGTTCTATCAAGGAAACACTACTTAGGCTTATAAG10.5813953488372093No Hit
CAATTGTAATGGTCTGTGGAATTACACGGAAACAGTTTTGTTGTAATAAT10.5813953488372093No Hit
AGATTACTTGACGAAGAAGAAAAAGCTCGATTAGATAGTTTCTAAAGTTA10.5813953488372093No Hit
GGGGATTGTGAGTTTTGTTTGAAGAAAACTGTTCTTGATTACAAGAAATG10.5813953488372093No Hit
AAACAAGATTTGATCAAATTACGACTAATCATCGGTCTAGAACAAGAAAC10.5813953488372093No Hit
ATAGAGAGACAAGTAAGTTACAGAGTACAACACTATGTACCACATAACCA10.5813953488372093No Hit
CGATCCTGACGTACTGGGCCCTCGAGGCCGCCCGCTGGCTCCGCGAGGAG10.5813953488372093No Hit
AGATAAGAGACATAAAACAAGAATCTTAGCTTCTGGTCAAGAGAGAGATG10.5813953488372093No Hit
CCATTATTGTTGTCAACAAAACCTTTAACACGGTCCATAGCAACCTCCAT10.5813953488372093No Hit
ATTCCAATCGACTAGGGGTGTTATGCCGATTCTTTTGAGTAATATATCTG10.5813953488372093No Hit
ACAACTAAGAGATCCATTCGAGGAACACGGGGACTAGTTAACGTAATCAG10.5813953488372093No Hit
CTTCTTCTTCCATTTCCCATTTACATCAAACAGCTATTTCTTCACCAATC10.5813953488372093No Hit
TTTTAATCTCGGACATTATAGTGAGCATCTAACATAAGAAGGAAGAATTG10.5813953488372093No Hit
GTACCAGCGGGATTATTNACCGTACCCTTTTTGGAAAATGTTAATAAGTT10.5813953488372093No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG10.5813953488372093No Hit
CTTCTAAGATCAGTAACCTGTAGGATAAAAAAAAACATTTACTAATATAA10.5813953488372093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)