FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_chi_056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_chi_056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATTGATCATAATGACTAAAGAAAGACTAAATAAACATCTAACGTC12.941176470588235No Hit
CCATAAGCAGCCACATGCCGTGAATCATGTGATTGGGCTCCTTAGGCATG12.941176470588235No Hit
TTGTAACACCAACCAGCAAGCACAAATTTGATTGTAGTACACTCACAGGA12.941176470588235No Hit
TTTATGCACCGCACCAAGATGACCCCACCCTTCTTCTCTCGAGGTTGGGG12.941176470588235No Hit
ACATAACAAACAGCATCAAAGAGTTTATATGACTCTCACTGATATATAAT12.941176470588235No Hit
ATGAGAAAGGTAGCCATGAAGACATTTACACATTCGCTGGCAAAGCCGAT12.941176470588235No Hit
TTAATCCCCATTCGAGCCTGGTTTCCAGAGAGAACAATATTACCTAGCCC12.941176470588235No Hit
GCCTAAAGCCGCCGCGATCATTGACCAGCTCACGGTCAATGCCGGCGTTT12.941176470588235No Hit
AAACTCAATCATACACATGAGATCAAGTCATATTCGACTCCAAAACACTA12.941176470588235No Hit
TGTCAATATATATCTCAATGTAATACCNTATTCTTTGTTATTCCATGGAT12.941176470588235No Hit
CTTCTTACTATTAGATAGTGTGCATAAATCTCATGATAGTTACCAAAAGA12.941176470588235No Hit
GTAGTGTTCAAGTACACAGGTTCCTCCAGCATTTATCAATGGCGGCATTG12.941176470588235No Hit
ACCCGATCTTGTAAGCCTAAGTAGTGTATCCTTGTTAGAAGACACAAATC12.941176470588235No Hit
GAATAACAGTGGAGAGATGCACCAAAGCAGCACCACCACCTGGAACTATG12.941176470588235No Hit
AAGGTGCCTAAAACAAGATAACTAGCAAAGAAAAATATTTCTAAACTAAG12.941176470588235No Hit
CTGTTTATACTAATATGAGTAGATTGATTGACTTAAGTCAGCAAAATGAT12.941176470588235No Hit
ATGTCTTCCTTTACCCAAGATCGAATCAATGGTACTCTCACCAAAGAGAC12.941176470588235No Hit
GGATATTCGTAGACGAAGAATAAAAAAACTTTCTTTATCTTTATATTTTA12.941176470588235No Hit
ACTATATGGTAACCGAACAGTTAACCGCACTATTAATCGCCTGTCTCTTA12.941176470588235No Hit
ACGTAGTTTTACGGTTTTGGCGGAAAAACGTGGTTTTACGGTTTTGGTGA12.941176470588235No Hit
CCATAGAGTCTCTAAATCTTTTGAGGATGAGGAATACAGAGTTCATTATT12.941176470588235No Hit
GACTTTGGCTACACCATGAAAGCTTTGAGAAGCACGAAGAATGTTGGTTA12.941176470588235No Hit
ACATTAAATATACCTCATCTAGATCCTCGAAGGTGTCCTCTCCAATTGTC12.941176470588235No Hit
ATAGAGAATTGTCTGAATCTCACTACAATGTCACCATATCAAAAAGTGAA12.941176470588235No Hit
CTCTAGCTATTTTCTTACGAAGCTTTGTTATAGCGTCTATAACAGCCTCT12.941176470588235No Hit
TAATTAACTTGGTCAACACCATGCATATTGACCTGACATGTATATTTATT12.941176470588235No Hit
GGATACAATTCTTACGCCTTTAAGATCTGGTTGCGGTTTAAGTTCTTATA12.941176470588235No Hit
CTATAAAAACAAAAAAAGCAAATAAGATATAAAAAAATTAATATAAAAAA12.941176470588235No Hit
CTGTTTACGTGTCCTTACGCAAATATGTTGATGCGTCTGTCAAATTAGAC12.941176470588235No Hit
TGGTAACTCTTGCTCATTTACATCAGAAAACCTCTCATTCTCACGAAATT12.941176470588235No Hit
GGAAATTATCTAAATCCTATAATATCCATTTCTGGAATGATGACCTAGGT12.941176470588235No Hit
ATATATCTTATCTTACAATTTACCAATATTTTCATACTTAATCCTATGAA12.941176470588235No Hit
GGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCTTGAAACACGGACCAA12.941176470588235No Hit
GGTTAGATAACTTTATTTTCAAATAACAAATACAAATCATTTTTCTTCCA12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)