FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_chi_045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_chi_045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTACATAGTCCGTTTTAGGGATTGGTGACTTACCCATTCAGTGACTT16.25No Hit
AATTAACTGAGAAGGATAGGTGGTAGGTAGTTCGATGCGCGAGCATGGAG16.25No Hit
GCTTAAGGAGCTTCTCCAAGACGAAGCATCAGTGGCGCAGCCGCGAGTGG16.25No Hit
GAATTTGAAGTCAATCACCTTGGATTTGTGAGACCAAAGTAGTGAATAAG16.25No Hit
ACTGATCATCGCCTTGGTAAGCTATTGCCTCACCAACTAGCTAATCAGAC16.25No Hit
GCTCTCTCACTTCCAGATTTTAGAATATCAATAGAAACAAAACTTTACAT16.25No Hit
TTCAAAGACCAATTCCTTCAACTATCATCCGCGTTACCGGAAAACAGATC16.25No Hit
CCCTCTGTTTAACCTCTCGCCGGAGAAACCGTAAGCCACGTCACGCCGAT16.25No Hit
CTATCAAAGCTTTGAGAAGCAAGAAGAAGCTTCGTAAGTGTTTGGGATTC16.25No Hit
ACCTAAAAGACATGGTCTCTCTTCACTCCTGGATCGGTCTCACCACTTTC16.25No Hit
CTATTCACGGAACGTGAGAAGCGAATGAATAATCATCTGCTTCCGGAAGA16.25No Hit
AAACACAATGGTATACAAGACATAANCTATTTTAACGGACTAGCAAATTC16.25No Hit
TCATTGTCGTATGATATGCTTGTAAAGATTTTTGACTAACAGTGAGGTTT16.25No Hit
ATTCTTCTTCTCTTCTCCGATCACGTCTATTTTGGGAGAGATGAGTCGAC16.25No Hit
GCACTTTCCCATTTGCTAATATTTTTTAGTTTCGTAATTATAGACTAAAC16.25No Hit
CTTGGGAGGTCCCAAATAAATGACTACTCGAGTCGGGGTTTTGAGCATAA16.25No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)