FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_chi_031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_chi_031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTTAAAGTCGAATATGACTTGATGTCATGTGTATGATTGAGTATAA50.3403675970047651No Hit
ATCATACACATCACATCTAGTCATATTTCACTCTAAAACGCTAACCAAGA40.2722940776038121No Hit
CTATATAAGTTACATAGTATATTCTTGAAGGTTCTTTTTCAATATAAAAG30.2042205582028591No Hit
TGTGTATGCAAAGCGAATTTCTGTTCTTAAGTATTCTAGTGCCAGGCCCG30.2042205582028591No Hit
TGATTTTACTATATAACTAGTGACTGTAATGAGTGGAGATAAAAAGTTTC30.2042205582028591No Hit
GTACTGTGATATCTAGTTAATCTTAATGATTATAAACCTTTGAAAAATCT30.2042205582028591No Hit
GTGTTCCCCTATGGGCATGGGCCGCCGTGCTCGGCGCAATTCTGCTCATG30.2042205582028591No Hit
GTGTATGATTGAGTATAAGAATTTAAACCGCAACCGGATCTTAAAGGCGT30.2042205582028591No Hit
TTATTGAGGTGTTTGATTCAGTAGGAGAAAAGTAAATTGAGAAGAGTGAA30.2042205582028591No Hit
AGTACATACAGGAAGTTCCACATGATTACGATATTCTTCCCATAAGGTTA30.2042205582028591No Hit
CCCTTAGCTATTGTTCTTGAGAAATGACCGGGTTTAGCCCATTCCTCGAA30.2042205582028591No Hit
AATTTATAGTAAGATGAAAATCCGTTGACTTTTAAAATCGTGAGGGTTCA30.2042205582028591No Hit
GTGGATTCGTATGAAAAAGAAGGGAGGAATGAGTCCTACACGTAGTCACA20.13614703880190604No Hit
CTAGTATTAGGACTATGATATAAGTAATTCCTGACATCTAGTCGAAAAGG20.13614703880190604No Hit
ATAAAGCACAGGCTGAAACAGGTGGAATCAAAGGGCATTATTTGAATGCT20.13614703880190604No Hit
TTACTGTTCATATGTTATGATATGAATATACCACACCAATTCGTTATGTA20.13614703880190604No Hit
GTCTTTGGCTTTGTGTCTTCTAACAAGGATACAATTCTTACGCCTTTAAA20.13614703880190604No Hit
GCTTTTTGTTGTTGTTGTTGCTAAATGGGTAAATATCATATAAAATGAAG20.13614703880190604No Hit
TGTGTGAGTATATGGAAGTCAGAGTTAAGCACATGGCAGATATTTTCCAA20.13614703880190604No Hit
GGGTTCAAACCAACCAGCGGTAGAGTTCAGGTAATGTGATATCTGTGGAA20.13614703880190604No Hit
CACTCTCAAATCAACCCAAAAATTCGACAGTTTGATGAATAGTTCTATCA20.13614703880190604No Hit
ATTCCAATGTCCTACAACATTTTCCTAACAAGTAATAAAAAAGTAATATA20.13614703880190604No Hit
ATGGCTAACTGGTCAAGGCACCATCAGCAAAGCTTTGTCAGCACTTTTAC20.13614703880190604No Hit
CCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTC20.13614703880190604No Hit
GTGTGAGGAGTCAAGTTAGAGACTTAAAACAAGCTCACTAGAGACTTAAA20.13614703880190604No Hit
GGATTCGAAAGTAAAAAAATTTAGGATCCAATAAGATATATATAAAAAAA20.13614703880190604No Hit
GTGTAGTTATTATTCTTTTTCGCTTTATCTCTGTAATATTATCNTCTAAA20.13614703880190604No Hit
GTGGAGATCCTTTTGGATTTCTCCAGGTACGTGTGACCGTATAACCTCGA20.13614703880190604No Hit
AATTTATGCAACAGCAGTCCAAACTATAAATTCTTTTGTTAAATTGGAAG20.13614703880190604No Hit
ATTATAAGCAAGCTAAGAGAATCAAGGAATCATCAATTCTTTCTCTAACC20.13614703880190604No Hit
AAAAATGGTTTGAGTTTAACTTAGAAACGGCATAATTGATCATAAATTAC20.13614703880190604No Hit
GGCTCCGAGAAGTAAAGAGCCAAATATACCAGTGTAGCAGTTGAGGGGAG20.13614703880190604No Hit
TTATCTTTCTGTTCAAAAAATTTAGTCATTACATTAGCTCAAATATACAT20.13614703880190604No Hit
CCCAAAAACCATATTTTGATTGTGCCTCAACTATATCAACTGTACTTAAA20.13614703880190604No Hit
CTGCACTAATTAATAATTTTCAAAACTCAGACAATAAAGAAAGAAAAATT20.13614703880190604No Hit
GGTACTGATATAGATGGTTTCGTCATTGAGCTTGAAAGGAGATGCCATCT20.13614703880190604No Hit
TCCCTTAGCCTATCAACAATTCAGCTCTGAAACCTGTTCCGAGACAATAN20.13614703880190604No Hit
GGAGGATAGATGGGGCGATTCAGGTGAGATCCANTGNAGATCCAACTTTC20.13614703880190604No Hit
GTATTATTCAGTATAAGAACTTAAACCGCAACCTGATCTTATAAGCCTAA20.13614703880190604No Hit
CCTATTATCAATTTGGCGCTGTTATCTTTTTCATTTTTTCTGAAAGTTAC20.13614703880190604No Hit
GAGCCATCCACCAAGTCATAATACATGATACTAATCTATAGATCCTAACT20.13614703880190604No Hit
GTCCAAGATCACCTTAAAAAAAATTAAGGTTACTGAATTAGGTTCAAAAA20.13614703880190604No Hit
CCATATGACACTGACAAATCATCTTCTTTCCCCACCGCCTCTGAGAACTC20.13614703880190604No Hit
CGGCTGATCTTGGTCAGTTCCAGCCGCGTCTTGTGGCCACTTTCGACTGA20.13614703880190604No Hit
ATTTACTTACGATACTTAGTTGACATTCATAAAAAGTATCTAATGAATTA20.13614703880190604No Hit
CATTAATAACAGGCCGTTTTGATCCTTTGGAACAACTCGATGAATTTAGT20.13614703880190604No Hit
ATATAATATATATCACCACAAATTAAGGTTGGAATACTTAGTAGCAACAA20.13614703880190604No Hit
TTAGAAATTTGGAGGTTTTATATGATTTAGTTCTTTTGTTTAACTTGATC20.13614703880190604No Hit
GTACACAAGCTGACGACTGAACTTCATTCTAGTTATTGGCTTGATCGGTA20.13614703880190604No Hit
CACAGGAAATATCCAAACTAAAATGGGTTGATCTCAGCCACTCGAGTGAG20.13614703880190604No Hit
CTCTTATGGAGTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAG20.13614703880190604No Hit
AATTAAAGAGATATGTTATTTATTTGTGAGTCTTATGGCATTTGATGCAT20.13614703880190604No Hit
GGAAAAAGACAACCAGACGAGTGCAAGTTCTGTTGAAAAACATGAAGAAT20.13614703880190604No Hit
CAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAGCCTCTCTCC20.13614703880190604No Hit
TTAGAAGACACAAAGCCAAAGACTCATATGGACTTTGGCTACACCATGAA20.13614703880190604No Hit
GTCTAATAAGATGGGACTGCAGGAAAGTAAGTGGAAAGTAAGAACACAGT20.13614703880190604No Hit
GTATTAGGGCATCCCATTAGTAAAGCAACCTGGACGGATTCATCAGATTC20.13614703880190604No Hit
GAGCAAACCTAAGGCCGACGGAAGAAAGATTTCATGGGCTAGCCTACGAT20.13614703880190604No Hit
ACTTCATTGATATTGATGTTTTCAATAGAAGCATGACTTATTGTTTAATT20.13614703880190604No Hit
AAATGTAGCACCAGTTTTGTCCCTAGAAAATTGGCGCATGTACTATAAAG20.13614703880190604No Hit
GGCCCTGCACGTTGCGGTGGCCGTAGGTGTAGATCCGCGGGTCGAACGGC20.13614703880190604No Hit
GTTGAAGAAGCATCCGGCACCATCTTTTAGATATGAAGGAAGGCTGGCGA20.13614703880190604No Hit
ATTAATAATGAACAGACGAAATCTGACGGTGTATGAATTGCCATGTGTAT20.13614703880190604No Hit
GGGTAAGAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCGAGTACCAGG20.13614703880190604No Hit
GAATGCTTGGGGTTACACTCACGAAGGTGCATAGTGAGAAGAGTAAGTCA20.13614703880190604No Hit
ATATATAAAAAAGAGAGGGAAGAGAGACCTTAGAACGACCATGGGGCAAG20.13614703880190604No Hit
ACATAAGCCACCTCTTGAAATCGTAGGGTTCGAAAGCGTCTGTTACGTGC20.13614703880190604No Hit
ATCATATACATGACATCAAGTCATATTCGACTCCAAAACACTAAGCAACC20.13614703880190604No Hit
ACCGCAACCAGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAGACA20.13614703880190604No Hit
CCATATGTCTTTATCTTTGTATCTTCTAACAAGGAAACACTACTTAGGCT20.13614703880190604No Hit
GAATATACCACACCAATTCGTTATGTATAGATGATGAGAAGATTCCATTG20.13614703880190604No Hit
GGATATAGCTTGGTAATGATTCTGTCTTGTGTCAGTTCGTCTGAGAGGAC20.13614703880190604No Hit
TGTTGGAGGATCCTGAGCTATGGTAGCTTGCTGAACCTCATTAGCGGGCT20.13614703880190604No Hit
TTTGAGAGCTTTTATTTCACATGATGAAGTGTTGCCTTACATATATATGT20.13614703880190604No Hit
TTATAGCTTTGCATCTAGCATCATCTAGCTCTTGTTTTGACTTCTTAGGA20.13614703880190604No Hit
CTTCTTTACGTGCTGGAAGACTCCCGTGCCTGATTGAATCATTATCAGCT20.13614703880190604No Hit
TTCATCGCCCATTCATCGTCCTGGTCGAGCATTCCGCCGTCCTGGTCGAC20.13614703880190604No Hit
CTATTCACTATCTTTATGGGGTAAAGAAGAAGAAAAAAAACTTAATAAAA20.13614703880190604No Hit
GATCTCTGTTTCTTCTACCATTCAGGAACCAAAGCTAGATCCGTCGTTGG20.13614703880190604No Hit
CGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCGTTCATCACCCT20.13614703880190604No Hit
TTATTAATTTGAGTGAAAGACTACTAACAAAAAGTTCTTATCCACCTTTA20.13614703880190604No Hit
GAACCTCCAAAGACTTGTCTTGTTGAACATGAGAAACTGTAAATTGTTAG20.13614703880190604No Hit
AGTTAAGATACATGAAAGTTTAAATAGACAACCATTTGTAAAAGATATAA20.13614703880190604No Hit
TGATATTATGTCTCAAAAGCTTATTCTGCAACTGAGTTGATGAGAAATCC20.13614703880190604No Hit
ATGACATCTGATCATCTTCTTCTGTTCTTCTTCTTCCATGCATGTTCTTG20.13614703880190604No Hit
GGATCCGGTGCATTAGTGCTGGTATGATCGCATACGTTAGTATATGCAAT20.13614703880190604No Hit
ATTTACATGAGATTGCTCTTTCTTATTGTTAAGCAAGTCCCCGAGAGGGC20.13614703880190604No Hit
GAGGAGGTAGGATGGTCAGTTGGTCAGATCTAGTATGGATCGTACATGGA20.13614703880190604No Hit
CTGGATCTTGATAACAATTTATCTTCACCACCAATAACAAAAGCTAGTAC20.13614703880190604No Hit
GTTGAAAAGCTTTTTCGTTATTTTAGTAAAACAATTTAGACATTAGGTTG20.13614703880190604No Hit
CTATACTATTGGGCATGTAATGCCTTGAATTCTATAATCAGGCGAGTGCA20.13614703880190604No Hit
AAATAGTTACATGTGCCTGTGTTTCTTTGTTTAAATTCTGAATCATTCTT20.13614703880190604No Hit
AAGCCAAAGACTCATACGGACTTTGGCTACACCATGAAAGCTTTGAGAAG20.13614703880190604No Hit
CCGATGAAGGAAATGACCCCGATATCTGGGCTGATTTAGTTGTTTTTGAG20.13614703880190604No Hit
CTGACTAATGAGGAGCATTTTGGAGGACTCTTGCCCAATTGGAGTCTCAA20.13614703880190604No Hit
GTCAACTTGTCATCTTCACTTAGCTCATCCATACCCAAAATTGCAATAAT20.13614703880190604No Hit
TTGAAAATGAATCGAATTATTGATTGTGTCATAAAGACCCTTATGCCCAC20.13614703880190604No Hit
GTTCACACCCTTCATAACTCTTTTCTTTGTCTTCATTGTCTTCATATTAA20.13614703880190604No Hit
CATATATATATATATATATATTAATAAGGGTTTTGAGTAATATTGTGTTC20.13614703880190604No Hit
CTTATAATGAAAACATCAAATATTCGGAAAAATAATACTCCACTGTTAAA20.13614703880190604No Hit
GCTAGGAACTGGAACGAGACGGGTAGCAAAGATTTCGAGTAGCACTTCAT20.13614703880190604No Hit
TGTCACGACTGCGTATGAATAGAGTTGTTTATATATTTAAAATATTTGTG20.13614703880190604No Hit
ACGTGTCAGAGATATGATGTAGTGAAGTTGACCAAGAGCCTTTACGTCCT20.13614703880190604No Hit
GGCCGCATCGGCCGGCGGCACGCCCACATGGTCGGCCGCCGCGATGGCGG20.13614703880190604No Hit
GAAGTATGTGGTTCATAGAGGATGGATAGATGCAGCTAATTACCTTTGGA20.13614703880190604No Hit
AAGGAAACACATCTTAGGCTTTTAAGATCGGGTTGCGATTTAAGTTGTTA20.13614703880190604No Hit
TTAATAGTGTTACTTGTGTATAACTGAACTTGTGAAAAATTGTATGATTG20.13614703880190604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)