FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGATCATAATCTATATGGGATTTCCTAAATTATTAATTGAAGATA36.122448979591836No Hit
GATAAGAAGTGTTATAAGAAAACTCTGCCCAAGCCAAATATTTCTGCCAA36.122448979591836No Hit
CATGTGAATTTTATTGAACATCTTGAATGCATAAACAAAACAGAGGAAAT36.122448979591836No Hit
GACTAATAGAGGTTCTCACCACTGCACATGCTCTTAGGGACCTAAACCCA36.122448979591836No Hit
GAAACTAAAAAACGATTAATTGTAAAAACAAATAAAGATATATAAGAATA24.081632653061225No Hit
ATTTAATCTAATGAGTTCACATTTAAGTCAATTCCATTTTAATAAAGTAA24.081632653061225No Hit
TTCTTACCTTCTTCATGTAATGCTTGTCAGTTAGGGAAACATGTCAAACT12.0408163265306123No Hit
CCTTTAACTTTTCATCTTCCATTCAATAGTTTTTAAAACATTTTCTTGGC12.0408163265306123No Hit
GTGTAAACCAACCAACATTTCATTGATTCAATTAATTCTTCTTTGATTTC12.0408163265306123No Hit
TATTAAATCTGTAACACAAGTTTAAAATACTTTAAAAAATTTTCTTTGTT12.0408163265306123No Hit
TTATAAGGCCGCAAGCAATGGCTTGTATGGCTTGACCTCAGGGCCGGCCC12.0408163265306123No Hit
GTTACCTTTCCAATGGAGGTGATCTCAAGTCATTTGAATGAGCCGTTAAG12.0408163265306123No Hit
ACATTATTTTAGCAAAACATTATTATTTCTTATAAGTTTCTTCTAGCAAA12.0408163265306123No Hit
TATATTTTATTGGACTCAATTCTGAATGTTGTGGTTTCGCAGGTTTTTTT12.0408163265306123No Hit
GAACCCAACCCTTCTTTGAAACACTTTGGTAGTTCTTCTGTATTGTATTA12.0408163265306123No Hit
GTATAAATGAACCGGTGTACGTGAAAAATCGGTGTACAAAGTGATTCGGT12.0408163265306123No Hit
TTCAACTCGTTTTCTGACATCTAGATCCTGATTAAGAGCAAAACTGTCAA12.0408163265306123No Hit
ACGAACAGCTTGCCGCGGCTCACGGTGTTGAGGCCATTGATGCAGCGCAG12.0408163265306123No Hit
CTGCTAATTCATCTCAGCGGAGCTTGCTCCAGAGTTGAGGTTGAAGGTTA12.0408163265306123No Hit
GTTGAGTGTTGTTCAACGAAGCTCTGAAATTAAGGAATAAGATCAAACCT12.0408163265306123No Hit
CTAATTAAGACATGATGTGTATATATATGTATATGTTTGAAGGTATAGAG12.0408163265306123No Hit
GGATTATAGGAATATGAAGTTTTGGCGTTATCTAGTGATACTTATTCACG12.0408163265306123No Hit
GTATTTAGCATTTATAAAGATTAATGTAATTTTACATTCATGCTAAAATT12.0408163265306123No Hit
GGGTTCTGGCGGCAACATGCTATGGGGTGGTGATCCCGCTTATGGGGTCA12.0408163265306123No Hit
GACCTACACACTCACAACACCTTGTACAGTCCAAAGCACAATTCTGGCTC12.0408163265306123No Hit
GAGTTTAATTGTTACATGTCTGTATGCCCCAAAGGTTAATAGAAATCAAA12.0408163265306123No Hit
ACAATGCCCAGATTCTCCAGCCACCAGTCCGGGCGCTGGATATGCCGCAG12.0408163265306123No Hit
ATAGGATGATCCTTAATATCTCCCTTCTTGCCACGACCAGAACAAGTATG12.0408163265306123No Hit
GTTATTGCTGCTGGGTTGGCTGTTGGGCTTGCTTCTATTGGACCTGGAGT12.0408163265306123No Hit
TTACTAATTTCGTCGGCTCTAATGGTTACCATGAGTATTGTCCTAATTCT12.0408163265306123No Hit
GTATACTCTGAGTTCTAGTTTTAATGTTTCTGATCTCTCTCCTTTTATTG12.0408163265306123No Hit
CTCATATACATGTGTTTTATTACTTTTTGTTGTTTGTGTTCTTATTGGTT12.0408163265306123No Hit
CATATATATATATGGACATCGTTTTGGTTATCCACCAAACCAAATACATT12.0408163265306123No Hit
GTTTACTTAGAAGCATTCGTGGATAAGATAAACTATGCATTGCTTTCTGG12.0408163265306123No Hit
GTAGTGTGTATGTTGTACTTTTACACTCTATACTAACTTAGAGTTAAACA12.0408163265306123No Hit
GAAATCCATAATAGAGTCCGACCACAACGACCGAATTGATTATCTTCATG12.0408163265306123No Hit
GTAAGGTTGTGAAACTTCTCTGGGAGAGCATCGAGGTTCATCCTCAAGGT12.0408163265306123No Hit
GAATTCAGAGACTTTGTCCATTGTGATGAAGGATTAGCGAAGAACGGAGA12.0408163265306123No Hit
ATAACTCAGCGGTAGAGTGTCACCTTGACGTGGTGGAAGTCATCAGTTCG12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)