FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAGTGGCTTATACCTAAACCCTTTTACTTCATCCTTATCTTCAAGT30.24213075060532688No Hit
GCCATGCAGCGACTCGATCCCCGTTCCAAGAACATGCAGCGGCTCCACGG30.24213075060532688No Hit
ACATAAATATAACAAGTTTAACAATATCCAAGGAAAATCAGAAACAAAAA20.16142050040355124No Hit
ACTTTACCATAATTTTCTTAGTGANACATAATCATAAATACATTTTTTTA20.16142050040355124No Hit
GCTTAATTGTATCCGTAATTTTCACTTACGGATACAATTAAGCTTTATAA20.16142050040355124No Hit
AGTTAGTATATTAAAGGATTGGTAGAGTTATGAGTAAGTTACTTATAAAA20.16142050040355124No Hit
GTAAAAACTAAAAACTCATCAATATTGTTAANAGAGGGCCACCATGTATT20.16142050040355124No Hit
AATTAATATAATAAGTTTTATCTTTTCTCCTACCTTCAGAAAAAAAAGTC20.16142050040355124No Hit
CATATGGTGTGTATGAATTGAGCACTATGAAATGAGATCCTCCTGCGTTG20.16142050040355124No Hit
GGATTCTAATTTGTAGATGAATTTTCTTATGAATTTCAAATTTAGTTTTA20.16142050040355124No Hit
TCTTATTACAACCAATGGGAGAACAAAAAGAAGAGTTAATTGAACTCATT20.16142050040355124No Hit
GTTATAGTATGTTCGAACGTGGAATTCATCATCCTTATTAATTTTGTCTT20.16142050040355124No Hit
CTTCCTCTGTTCTTTTTCGGCTAAGATGAAGATCTAATCACGACAACAAA20.16142050040355124No Hit
CCCTCTCACAACCCGTCCTGCCAATGGCCTTCCTGCTGGCGCTGAGACGA20.16142050040355124No Hit
TTAGTGGAGAGATGGCAAAGTTGTAATTTTCCAAATAAGATAAATATGTG20.16142050040355124No Hit
GTCTCTTGGTTTGAAGTAGATCTGAAAGCCATAGGCAATGCTTGCCCACA20.16142050040355124No Hit
CGAGTAACTTTTTCACCAAGTTTTCTAATAGGCTAAAGCATATCATCATT20.16142050040355124No Hit
GATGCACTTATCACATTCATTGATACTGAAACCATTTGACATCATTACCT20.16142050040355124No Hit
GATTTGACCTGCACATTTTATGAATAAGGGTTAATCCTGCCAAAAAAAAA20.16142050040355124No Hit
ATCCAAAAATTTGCTCTTTTCTAATATTTAAAGATAAGAGAGTTTTTACT20.16142050040355124No Hit
GTAAAGAAACGTACCTCGGCAGTTTCTCAAAGTTTTTGTGACCCATCCAT20.16142050040355124No Hit
AATATGGATCAAAAAGGAGCAAATACCCGTTTTTGAGGCAGTTTCAAAGC20.16142050040355124No Hit
GTCAAATATGCGTTTTCGCACAGTAAGGCACTTTATACTTTGATTTCCGC20.16142050040355124No Hit
GTATAGGACCCATTTAGATTTTTTTTTGTTAGTACCCTTTTTCGGAGTTG20.16142050040355124No Hit
ATCCTAAAGAGAATCCAGTACTTCNTGGTCGTGAATATCTGAATAGGGCG20.16142050040355124No Hit
CTACAATAATATATCATGTAGTATTTTTTTTTGTATCTTAACTGTTTTCT20.16142050040355124No Hit
CTTATTTGCTGAGGAGAAACTAATCCAATTCGGAGTTGTTGATGTTTATA20.16142050040355124No Hit
GCATGGAAGACAGTATGGCAGAAGACACAGATCAAGCTAGTAAAGCATCG20.16142050040355124No Hit
GGCGATACCCTTATTTTTATAAGTTGTAGCAATGTAATAGCTAATATTCA20.16142050040355124No Hit
TAACTAATCAATTGAAACCTCAGATTCATACGAGAACCACGATAATTCAA20.16142050040355124No Hit
GTTCAGCATCTTGGACAAGCCAACCCTTAGAAGGTATCGTTAAAAGATCA20.16142050040355124No Hit
GGGATAAAAAATGGATCATTGGCACCCCAAATCGCCAATGTGGGTGGCTG20.16142050040355124No Hit
TAGCAAGAGCACGAAAAATTGAGCGTTTCTTATCACAACCCTTTTTCGTA20.16142050040355124No Hit
AGTTCCAGCTTAAACACAAATGGAAATCTAAACAATTACAAACAGCTTCA20.16142050040355124No Hit
GTAACAAGGTAGCCGTACTGGAAGGTGCGGCTGGATCACCTCCTTTTCAG20.16142050040355124No Hit
ACACAGCTACAAGCCTAAAAACCTAATGATTGGTCAACTAGCATATGGAT20.16142050040355124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)