FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATTGAACATTGTCATAAACGAGTATGGGAAGATTATAAATGGTAC24.878048780487805No Hit
GGTTTTTGGAATGGAGAAAGCTTCATCGCCGTCTTCTTCACGAAGGTCTG24.878048780487805No Hit
GATTCTGATTGACAAATCCAACGCACACGAAAGAATCCCGTAGTCTCAGT24.878048780487805No Hit
ACCTAAATAGGGATATAGATTTGGATACTGGATTTATAAATGGTTGGATT12.4390243902439024No Hit
ACAATGTACCACGCATGACACCCTTCAGAATCACATCAGAACCTTGACAG12.4390243902439024No Hit
ATATAGTAGATGGACTTATATTTTAATATTAAAGACTAGTTTAAGTTTGA12.4390243902439024No Hit
GTGAAAGGATGTTCATGCAGATTAATGTTAGGGTTTTTATGAGATTGATC12.4390243902439024No Hit
TATTATGACTACACAGTCGCTGCAATTTGATTTTAAGACAATTGAAGCTG12.4390243902439024No Hit
ATTAAAAAACCGCGTTTCTTTTCGAAAAGCAATGAAAAAAGCTATTGAAT12.4390243902439024No Hit
CATTGTATACATCTTATCTCTTAGTGGGAATAAAATTAACCAAAAAACAT12.4390243902439024No Hit
ACCTTGAAGATAACAAAAGATATGCTGAATCTCATCTCAATGTTAACATG12.4390243902439024No Hit
GCATACAACCCTTTGATTCCACGAGTTTGCACTATACTCATAAATGCTGA12.4390243902439024No Hit
TTTTAGCTCCAAGGAAGTGAAAACCTGAAAAGACACTAAAACCAACCAAA12.4390243902439024No Hit
ATGAAATTACGTCGATACTTCCACATATTATGGGTAACGTAGGAGACATC12.4390243902439024No Hit
GAACCCCTTACAAACTAATGGATGTAAACAAATAGTGCGTCCTTCCACTA12.4390243902439024No Hit
AGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACC12.4390243902439024No Hit
AATCTCACCTGCATTCTTTGTCAATCTTAAGGCAAGCTTTATGCATATCA12.4390243902439024No Hit
GTGTATCTTATTGGATGGATAATTGGGTCACAATTAGCCACGAGTCACGA12.4390243902439024No Hit
CATTTTCAAGAAACTCTGATGCTTTTTGGGGATCAATCCTGCAAAATATT12.4390243902439024No Hit
GGTCCCTTGAAACTCGTGGTCTCCAGTTTGTCCGTTGTTTGAAAAACAGT12.4390243902439024No Hit
GATTTCGTAGTGAACAAAAAGCTCAAGATGCTATCACTTACTTTAACAAG12.4390243902439024No Hit
CCTTAGGGGATCTACTCTATTACATAAGTAGATTCCTAAGATTTATCTCA12.4390243902439024No Hit
ACCTCGTCCCGGACCATCGCAAGGAAAACTATACCCGAAATCCTTTTTAT12.4390243902439024No Hit
TGTCTATATTTAAATTTTGTTTTCGATCGTGACACGTATCAAGACTATCG12.4390243902439024No Hit
GAACTATACAGTGGACTGTGGGGTTTAAAAAATGGTTTATGACGACTAAG12.4390243902439024No Hit
CTACTAGCTTTGCAGAAGAATGGGCTGGGTACTATGTGCATCAGTATTAT12.4390243902439024No Hit
GATTTACATAGTGGGCAATACGTTTTCTTGTTGATTAAGATATAGGGTTT12.4390243902439024No Hit
AGATAATATTCTCATACGAGAGGTGGTTTATTTGTTTATAGTGCCCTGAG12.4390243902439024No Hit
GTAAGTTTCACGCGCGGCAACTTTCCAAGGCTCGGTTCGTCAAGTTGTGA12.4390243902439024No Hit
CATCTTATTCTCATTTCCTTTTGTTAAAAACCAATTTTTTATTTGTTCTA12.4390243902439024No Hit
AACTATATTTGATTAGGACCTAAGAGCATAATGCTATACACCACCACTTT12.4390243902439024No Hit
CTAGAAGGAAGCTGTCGATGATCATTTGTTGTTTTCTTATCGGAAAAAAT12.4390243902439024No Hit
CTGGAAAATACTGAGACATGAGATATTGTCTCAAAACCAGATAGGAAGAA12.4390243902439024No Hit
ACAGAAAGTCCTAATATATCCAAAAATAATAAAAAGGAAATAAAAATCAG12.4390243902439024No Hit
GCTTTACACCATATATTTCTCTTCAGGCCTTTACCACTCTTTTCGTTTTC12.4390243902439024No Hit
CTCTTAGAGCTTGTAACTGGAAGGAGACCAGTTGATCAGAGTCAGCCTCT12.4390243902439024No Hit
GTTTAGGTTAACCCAATCATCGATGCAGTTCGTAGCAAAGGAGATACTGC12.4390243902439024No Hit
ACAATCCTCTTCCCGCTGAGCCTCCTTTCTTCTCGGTCCACAGAGACAAA12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)