FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCTGATTGTCCGTATTTTGACAGAGTGTCAAATCTGGGACTAGAAAA30.2437043054427295No Hit
AATTAATACTTAAAATACAATAAAAAATTTATTTTATTTTCAAAAACAAA30.2437043054427295No Hit
CATCCAGACCCTTTACTAGACAATCATTACTGTATTTTTTTGGTGTTTTA30.2437043054427295No Hit
ACTCCTTAGGTGTACCAGCATCCAGGGGATCATGAGGATTCCTTTGTGTT30.2437043054427295No Hit
GTATAGTGTGTAACGTACCATGAGACCAAGAGTGAACATATTGAAACCGC30.2437043054427295No Hit
CCGCAAACTTCTCCTCGTAACCCTTCGCCAAACTCTCGGCTGCGGTGCAA30.2437043054427295No Hit
CTATAGAACAATGCATTTTTGAATGCTTATTGGCAAAAATATACAAGTAC20.16246953696181965No Hit
AGGCTTCCCTTTAATAGTATAGATTCTTCTTGATGATTTTCCTCTTTAAT20.16246953696181965No Hit
ATATATATATAAGGCATCCTCCGGATAATTCAAATCTAAGCAATTAGATG20.16246953696181965No Hit
TGTCTACGCTTCCGGAGAACTTCATATGAGAATCAACGGCTACTACAGGA20.16246953696181965No Hit
CTCTAGAACCAACTCAGAAGCAGCAAAGCTTCTAACCGGGAAGACACTTT20.16246953696181965No Hit
ACTGAGGCGAGAGTTCGATGACGAAATCCGCCAGTACTTGCGCCTTTGCA20.16246953696181965No Hit
GTACCATTATGGCAGAGGATTCGGGTAAAATATGCACTTCCTCAGGAATG20.16246953696181965No Hit
TCACCTGAATAGCTTGTTTTATTTATCAACTGATTACTGTAGACCACTCT20.16246953696181965No Hit
GTAGAATCAGAAAGCGATGTTACTGAAAATAATAAAAACCATATAGACCT20.16246953696181965No Hit
TTTCTAAAAATTGGAACAAGATTGTAAAGGCATAAAAGAGTTTATCTTTA20.16246953696181965No Hit
GTGTTAGAAGGAGTTACATATGTATAGAAACCTTAAACACAAAGATAAAT20.16246953696181965No Hit
GTTCATCCTTCGGAACCATATCACATCCCAGATCTGATGAAATAGGATGA20.16246953696181965No Hit
GGTAAGACGCCATCAGAGCGTTTAGTGTTCACAGCAAATTATGCAAACTG20.16246953696181965No Hit
TATATATATATTAATGATATATTTGAAATATTTGAAATATAATTATATAT20.16246953696181965No Hit
GACAATGACAATGAGTAGGACACATATTGAAGATAAAAACTCAGAAAATA20.16246953696181965No Hit
CTTAATTAGCATCTTCGAGAAAATAGAATAATATTTCAAAAATGTTCTTA20.16246953696181965No Hit
AAATTGAGTTATATTAACATAAAAATCAGGTGGATGATATCAAAATCGAT20.16246953696181965No Hit
ATACAAAAGACTCTAAAACGAATATGTATATCATGGCTAAAAACCATAAA20.16246953696181965No Hit
TCTTTGCACAGTAGCCTTGTGAGCGGTCTTGCAAGTTTTGAGAAGTCTTT20.16246953696181965No Hit
GTTTATATGGCGGAGGTTTTGAAAACTGGGGTGAGACTTAGCCGTGGAGA20.16246953696181965No Hit
GATCGAAGGAATCTTAACGAGTGCCCCAACGACGTCACTTCCACCGTTAT20.16246953696181965No Hit
TGACTATAGAAGAACTCTTGCATTCCCACCAAAGTGAATACATCAGCCAT20.16246953696181965No Hit
GTGGTTCGGGAAAAAGCACTGTGATCGAACTGATCATGAGATTCTATGAT20.16246953696181965No Hit
ACCCAGAACAATCAACCGTCGTGGGACCCATTGTTTTCTAATGGGCTAAT20.16246953696181965No Hit
GTTCTAGACCACAATAAATAGTTGCTCCCTTTGAGAACAACTGGTAGAGT20.16246953696181965No Hit
GTATGCCAATATGCATGAGAGTGAGCGAAGCTGAAGGCACGCAAGAGATT20.16246953696181965No Hit
AATGTATTATACGCTATCCTATTCTATTTTCCTACACGAATTAAGTTGAC20.16246953696181965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)