FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTAACGTAATATATAGAGTATTTTTGAAAAAAGGAAAGACAAAGAAAA25.88235294117647No Hit
GTTGTTGTATTTGATTTCTATTTGTTGTTGTCGTCGTATTTGTTTGTTTT25.88235294117647No Hit
GATTCACATAAGTTGTAGCAACGCTTAAGGACTCAAAACTCTAATTTGAG12.941176470588235No Hit
GACCAATTGTGCGAATCAACGGTTCCTCTCGTACTAGGTTGAATTACTAT12.941176470588235No Hit
ATTTTTTTCTATATGGCTGGCTCGTTGATTTGTACCGGGGGTAAATTTGA12.941176470588235No Hit
TGCTGAAATTGACTCATATTACAAAAGTTGTGTAGGCGGGAAAGAACAGC12.941176470588235No Hit
TGTAAAAATAAAAATTGAAAATGCATAGCTAAACTTTTTTGGAGGCAGAA12.941176470588235No Hit
GTCCCTAGCTTAGACCATAAGGTTTTCCAAAAATAACTCAAGAAATTAGT12.941176470588235No Hit
CACAAATAAATAAATTATAGACAAATAAATAAAACATATACTATAAAATC12.941176470588235No Hit
TGTTTATGCTTTATTCTAAATATATTATAATCTTAATTTTAATTCCTTAT12.941176470588235No Hit
CTACCACACAGCACACTCAGTATGGACATTGGTTCTAAATAAGGGGAGGA12.941176470588235No Hit
GAAGTATCCAGAGGCAGTGAAGCATTATTCAGAAGCAATCAAAAGAAATC12.941176470588235No Hit
CAATCACCATATCTCTGTTAATCTCACACAAATAAAAGTTTGTCTCGCCA12.941176470588235No Hit
TCATCCCTTTCTTGTTGTTTTCAACCATAAAACATAATAATCTATCAACC12.941176470588235No Hit
ACTATGGGGTTTCTTTGAGCNTTTCATTAATGTGTCAACGTTNTTGGATG12.941176470588235No Hit
AGGTAGAGCACCTCGTTTACACGTGCGCCAAAGTTTTTCAGGGGAATCCA12.941176470588235No Hit
GTTTACTACAGATGCTATTGAGGTCTTGAAGAGGAAAAAAAGTAGAATGG12.941176470588235No Hit
ATATTGTATATATTACAAAGCATGAAAATACATATTTATAGTGTAATGAA12.941176470588235No Hit
ATCTATTCCTCATCAGCTAACATCTTTAAACAGATTCATGAATACAAATC12.941176470588235No Hit
AAATCAATCAATCAATCCAATCTATTTATTTTTTTGAGATGTTAGAAATA12.941176470588235No Hit
ATAACGAATCTTGCAACACACAAATGATGGAGGATACACTAGACACCATT12.941176470588235No Hit
GTTCACAACCCACAAGTGATTTATATTGTTAACGAAATTTTATTGTTAAG12.941176470588235No Hit
GTTTTAAGTTATAAAATGCAATGCTTATGAACATGCATAAACTCTCAGCC12.941176470588235No Hit
AGAGTGATTTGATAATCTGAGAATGGAGCTAAAGAGTGTTAAAGACGCGT12.941176470588235No Hit
TATCATGATTGAGGGACAAGTTTTAAGAGATAAAAATGATCTCTTCTTCC12.941176470588235No Hit
GAGAAAAACTGAAAAGACACTAAAACCGGCCAAAGGAACACAAAACCTAA12.941176470588235No Hit
ATAAAATAGACATCATAATGTTATTGCATTTTTCATATAGGCTCAGGATC12.941176470588235No Hit
CACTTTCAAGTCATCATCGTTCGTCTCTATTGTCGCTCTTGGCTTTCTCT12.941176470588235No Hit
ATTTAAATGAGAAAAACTGTTGAAAATGCCACTCGACCACTATGGTCGAG12.941176470588235No Hit
GGATGAGTATGATACCTTGAGTTATAATGCTTGGCTGAATGTGGAGATTA12.941176470588235No Hit
GGAGAATGAGACTCATAGAGGCAGAAAACGAAACGGTACAACTAATAACC12.941176470588235No Hit
CATTTTTTTTTCCCATTTTTGTTCATCATTTATTCTCATTTTCCCAAAAA12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)